外源褪黑激素对罕山白绒山羊绒毛生长相关基因表达差异的影响

doi:10.12160/j.issn.1672-5190.2019.09.003

Animal Husbandry and Feed Science ›› 2019, Vol. 40 ›› Issue (9): 11-16.doi: 10.12160/j.issn.1672-5190.2019.09.003

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Effects of Exogenous Melatonin on Expressional Differences of Cashmere Growth Related Genes in Hanshan White Cashmere Goats

Lichun¹, LI Jin-quan², ZHANG Wen-guang², WANG Si-zhen¹, WANG Guo-fu¹, GAO Shu-xin¹, FU Shao-yin^{3, 4}

1.College of Animal Science and Technology,Inner Mongolia University for Nationalities,Tongliao 028000,China;
2.College of Animal Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China;
3.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
4.Inner Mongolia ATCG Bioinformation Institute,Hohhot 010020,China

Received:2019-09-06 Online:2019-09-30 Published:2019-12-17

Abstract

Abstract: The aims of this study were to screen the key genes associated with growth and loss of cashmere in skin follicle in Hanshan white cashmere goats, and to reveal the effects of exogenous melatonin （MT） on the expression of genes related to cashmere growth in Hanshan white cashmere goats. A total of 10 Hanshan white cashmere goats aged 20 months with average body weight of 33.3 kg were selected as experimental animals and were divided into two groups. The goats in the experimental group received a subcutaneous implantation of MT in skin behind ear at a dose of 2 mg/（kg·BW） every 1 month from December 2014 to December 2015. The skin tissues of the goats in the two groups were collected in September 2015 for RNA-Seq sequencing, and the screened differential expressed genes were subjected to function annotation and enrichment analysis. A total of 475 differentially expressed genes were screened between experimental group and control group, of which 392 were up-regulated and 83 were down-regulated. KEGG enrichment analysis showed that the differentially expressed genes were mainly involved in leukocyte endothelial migration, chemokine signaling pathway, fcγ-R-mediated phagocytosis, phage, hematopoietic lineage, complement and coagulation cascades, cytokine-cytokine receptor interaction, NF-kappa B signaling pathway, PI3K-Akt signaling pathway, etc. PI3K-Akt signaling pathway may play an important role in MT-regulated skin follicle development. Our results provide a basis for further study on the molecular regulative mechanism of MT in skin follicle growth.

Key words: melatonin, Hanshan white cashmere goats, differentially expressed genes

CLC Number:

S827.912

Lichun, LI Jin-quan, ZHANG Wen-guang, WANG Si-zhen, WANG Guo-fu, GAO Shu-xin, FU Shao-yin. Effects of Exogenous Melatonin on Expressional Differences of Cashmere Growth Related Genes in Hanshan White Cashmere Goats[J]. Animal Husbandry and Feed Science, 2019, 40(9): 11-16.

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Effects of Exogenous Melatonin on Expressional Differences of Cashmere Growth Related Genes in Hanshan White Cashmere Goats

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