Abstract: In order to provide reference for the selection of appropriate methods for aflatoxin B₁（AFB₁） detection in laboratory, the detection performance of AFB₁ in feedstuffs by HPLC with photochemical derivatization, HPLC with trifluoroacetic acid derivatization and LC-MS/MS were verified and compared in this study. The results showed that HPLC with photochemical derivatization and HPLC with trifluoroacetic acid derivatization had good linear range and correlation coefficient （R≥0.999 97）, the recovery rates of AFB₁ ranged from 88.4% to 104.6%, and the precisions were below 5%; the correlation coefficient （R） of LC-MS/MS method was ≥0.999 00, the recovery rates of AFB₁ ranged from 85.3% to 96.8%, and the precisions were below 3%. The combined data suggest that for the determination of AFB₁ in feedstuffs, HPLC with photochemical derivatization has the advantages of simplicity of operation, high sensitivity and good stability, but had higher cost; HPLC with trifluoroacetic acid derivatization has lower cost, but is susceptible to the interferences from matrix in sample, and the operating procedures are complicated; LC-MS/MS has higher qualitative accuracy, but has lower quantitative accuracy and the highest cost in the three methods.

Key words: HPLC, LC-MS/MS, photochemical derivatization, trifluoroacetic acid derivatization, AFB₁, feedstuffs