RFRP-3对大鼠采食行为和生长性能的影响

doi:10.12160/j.issn.1672-5190.2020.01.001

Abstract

Abstract: In order to investigate the effect of RFRP-3 on the feeding behavior and growth performance of rats, a total of 30 clean-grade SD rats with similar weight were selected and randomly assigned into the following three groups: a control group received a 200 μL saline solution per rat per time by intraperitoneal injection, a low-dose RFRP-3 treatment group received the 200 μL RFRP-3 solution with concentration of 1 μg/100 μL per rat per time by intraperitoneal injection, and a high-dose RFRP-3 treatment group received the 200 μL RFRP-3 solution with concentration of 10 μg/100 μL per rat per time by intraperitoneal injection. There were 10 rats in each group with half male and half female. All of the experimental rats received intraperitoneal injection twice daily for consecutive 14 days. During the whole experimental period, the feed intake, feeding behavior and body weight of the experimental rats were observed and recorded; the feed to gain ratio and Lee index were calculated, the serum was isolated from the blood samples, the blood lipid related indicators were determined, and the expression level of hypothalamus appetite related factors was assessed. The results showed that compared with the control group, the intraperitoneal injection of high dose RFRP-3 resulted in an extremely significant (P<0.01)increase in feed intake in rats in light-environment, and the feeding behavior was mainly manifested in a extremely significant (P<0.01) increase in feeding frequency and an obvious decrease in satiety; in terms of growth performance, the RFRP-3 treatment groups had a very significant (P<0.01) increase in body weight and a significant (P<0.05) reduction in feed to gain ratio; for obesity indexes, the Lee index of the rats was increased significantly (P<0.05) after receiving the intraperitoneal injection of RFRP-3, and the serum contents of triglycerides and total cholesterol were also very significantly (P<0.01) increased; the expression level of NPY mRNA in the hypothalamus in RFRP-3 treatment groups was increased significantly (P<0.05), while that of POMC mRNA was decreased significantly (P<0.05). In conclusion, intraperitoneal injection of RFRP-3 can improve the feeding behavior and growth performance of rats, and this physiological function may be achieved via regulating the appetite-related factors in hypothalamus.

Key words: rat, RFRP-3, feeding behavior, growth performance, NPY, POMC

CLC Number:

Q571

HUO Kong-lin, ZHANG Duo-ni, SONG Xing-xing, HU Wen, ZHANG Xin, ZUO Jian-yu, LUO Rong-rong, XU Wen-hao, CHEN Lei, LI Xun. Effects of RFRP-3 on Feeding Behavior and Growth Performance of Rats[J]. Animal Husbandry and Feed Science, 2020, 41(1): 1-6.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal30.magtechjournal.com/xmysl/EN/10.12160/j.issn.1672-5190.2020.01.001

https://journal30.magtechjournal.com/xmysl/EN/Y2020/V41/I1/1

References

[1] TSUTSUI K, SAIGOH E, UKENA K, et al.A novel avian hypothalamic peptide inhibiting gonadotropin release[J].Biochemical and Biophysical Research Communications,2000,275(2):661-667.
[2] HINUMA S, SHINTANI Y, FUKUSUMI S, et al.New neuropeptides containing carboxy-terminal RFamide and their receptor in mammals[J].Nature Cell Biology,2000,2(10):703-708.
[3] UBUKA T, INOUE K, FUKUDA Y, et al.Identification, expression, and physiological functions of Siberian hamster gonadotropin-inhibitory hormone[J].Endocrinology,2012,153(1):373-385.
[4] 雷治海,李月.促性腺激素抑制激素的研究进展[J]. 畜牧与兽医,2008,40(9):96-99.
[5] CLARKE I J, QI Y, PUSPITA S I,et al.Evidence that RF-amide related peptides are inhibitors of reproduction in mammals[J].Front Neuroendocrinol,2009, 30(3):371-378.
[6] TSUTSUI K.A new key neurohormone controlling reproduction, gonadotropin-inhibitory hormone (GnIH):Biosynthesis, mode of action and functional significance[J].Progress in Neurobiology,2009,88(1):76-88.
[7] ARBLE D M, SANDOVAL D A.CNS control of glucose metabolism:response to environmental challenges[J].Frontiers in Neuroscience,2013,7:20.
[8] GOEBEL M.Central nesfatin-1 reduces the nocturnal food intake in mice by reducing meal size and increasing inter-meal intervals[J].Peptides,2011,32(1):36-43.
[9] BERNARDIS L L, PATTERSON B D.Correlation between ′Lee index′ and carcass fat content in weanling and adult female rats with hypothalamic lesions[J]. Journal of Endocrinology,1968,40(4):527-528.
[10] TACHIBANA T, SATO M, TAKAHASHI H, et al.Gonadotropin-inhibiting hormone stimulates feeding behavior in chicks[J]. Brain Research, 2005,1050(1/2) :94-100.
[11] McCONN B,WANG G,YI J, et al. Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks[J].Neuropeptides,2014,48(6):327-334.
[12] SCHNEIDER J D, TOKACH M D, DRITZ S S, et al.Effects of feeding schedule on body condition, aggressiveness, and reproductive failure in group-housed sows[J].Journal of Animal Science,2007,85(12):3462-3469.
[13] ANJUM S, KRISHNA A, TSUTSUI K.Possible role of GnIH as a mediator between adiposity and impaired tes ticular function[J].Frontiers in Endocrinology,2016, 7:6.
[14] 王晓晔,霍孔林,肖凯,等.GnIH在陆川仔公猪消化系统及气管的分布定位[J].华中农业大学学报, 2016,35(3):89-93.
[15] 李卉,罗明秀,宋辉.GnIH基因在小鼠各个组织中的表达[J].宁夏农林科技,2017,58(10):24-26.
[16] UKENA K, TSUTSUI K.Distribution of novel RFamide-related peptide-like immunoreactivity in the mouse central nervous system[J].Neuroscience Letters, 2001,300(3):153-156.
[17] UBUKA T, LAI H, KITANI M, et al.Gonadotropin-inhibitory hormone identification, cDNA cloning, and distribution in rhesus macaque brain[J].Journal of Comparative Neurology,2010,517(6):841-855.
[18] DARDENTE H, BIRNIE M, LINCOLN G A, et al.RFamide-related peptide and its cognate receptor in the sheep:cDNA cloning, mRNA distribution in the hypothalamus and the effect of photoperiod[J].Journal of Neuroendocrinology,2008,20(11):1252-1259.

Effects of RFRP-3 on Feeding Behavior and Growth Performance of Rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Xiaodong, YU Erru, HONG Liping, SHANG Yishun, PEI Chengjiang, LUO Jinping, CHEN Caijun. Effects of Different Mixing Ratios of Corn Straw and Spent Flammulina velutipes Substrate on Nutritional Quality and Mycotoxin Content of Fermented Total Mixed Ration [J]. Animal Husbandry and Feed Science, 2024, 45(2): 20-28.
[2]	ZHANG Xiaoyan, ZHANG Jian, CHEN Xiang, Chao Lemeng, XI Yaqin, JIN Jing, FENG Caixia, Hunusitu, ZHANG Tianyu, WANG Yaru. Effects of Different Remediation Measures on the Restoration of Degraded Grassland [J]. Animal Husbandry and Feed Science, 2024, 45(2): 45-49.
[3]	ZHANG Ying, WANG Lifang, ZHANG Tenglong, ZHONG Huachen, SONG Jie, GUO Chenyang, LIU Jialin. A Comparative Study on Milk Yield and Quality of Dairy Cows in Different Regions of Inner Mongolia [J]. Animal Husbandry and Feed Science, 2024, 45(2): 82-87.
[4]	ZHANG Junli, HOU Pengxia, LIANG Xiaojun. Effects of Pumpkin Seed Shells on Growth Performance,Serum Biochemical Parameters,Immune Indexes,and Hormone Levels of Simmental Crossbred Cattle at Different Growth Stages [J]. Animal Husbandry and Feed Science, 2024, 45(1): 20-24.
[5]	TANG Yiying, LIU Jinsong, XIAO Shiping, LIU Yulan. Effects of Dietary Supplementation of Different Plant Essential Oil Preparations on Growth Performance,Intestinal Digestive Enzyme Activity and Serum Immunoglobulin Level of Weaned Piglets [J]. Animal Husbandry and Feed Science, 2024, 45(1): 25-30.
[6]	XU Cong, LI Jing, LUO Xiaoyan, YANG Qiunan, WU Mingwei, SONG Shiya, ZHU Yukun, YANG Xi, WU Zhilei, LI Hongxia. Investigation and Risk Factor Analysis of Intestinal Parasitic Infection in Sheep in Yuxi City,Yunnan Province [J]. Animal Husbandry and Feed Science, 2024, 45(1): 102-106.
[7]	LI Ashi, LIU Pan, ZHANG Xinnan, HU Yulu, LI Yujie, ZHAO Yang, WU Shangze, HE Jianzhong, ZHANG Mengdi. Effects of Gossypol on Growth Performance,Serum Antioxidant Capacity and Renal Pathological Morphology in Male Broilers [J]. Animal Husbandry and Feed Science, 2023, 44(6): 22-30.
[8]	ZHOU Yunhao, LI Xiaodong, SONG Li, SHANG Yishun, LIU Fengdan, PEI Chengjiang, LI Shige, LIU Junlin. Isolation,Biological Characteristics and Enzyme Activity Analysis of Cellulolytic Bacteria from Spent Flammulina velutipes Substrate [J]. Animal Husbandry and Feed Science, 2023, 44(6): 31-40.
[9]	LI Lianhong, HAN Xueqin, LUO Huiying, DENG Hongshan, LIU Jindi, LIU Yun, JIN Jie, ZHANG Meiyan. Research Progress in Bioactivity of Cymbopogon citratus and Its Application in Livestock and Poultry Production [J]. Animal Husbandry and Feed Science, 2023, 44(6): 57-65.
[10]	WANG Jingran, LI Pengfei, LIU Miao, TAO Yanlin, LIU Yanan, ZHU Shufen. Advances in Respiratory Diseases and Respiratory Tract Micro-ecology [J]. Animal Husbandry and Feed Science, 2023, 44(5): 30-38.
[11]	SHI Zhidan, GUO Chengyu, FANG Yongyu, YI Fengyan, LIU Sibo, HUANGFU Haiyan, SUN Hongxia, DING Haijun. Advances in Molecular Research of Giant Juncao（Cenchrus fungigraminus）and Its Application in Ecological Restoration [J]. Animal Husbandry and Feed Science, 2023, 44(5): 77-82.
[12]	DUAN Jianhua, WANG Xiujun, ZHAO Jing, LI Cuizhi, LYU Zhiyong. Inter Laboratory Comparison Analysis on the Detection of Lead, Chromium, Mercury and Arsenic Contents in Milk Powder [J]. Animal Husbandry and Feed Science, 2023, 44(5): 100-107.
[13]	QIN Haixia, HAN Fei, DAI Yanping, LIU Gaiping. Expression of Caspase-9 Protein in Leydig Cells of Rats with Sub-chronic Arsenic Poisoning [J]. Animal Husbandry and Feed Science, 2023, 44(4): 17-21.
[14]	WANG Jing, TIAN Yonglei, MU Zongjie, WANG Yongrong, BAI Chunli. Effects of Nitrogen Fertilizer Application Rates on Contents of Protein and Cellulose in Forage Oat [J]. Animal Husbandry and Feed Science, 2023, 44(4): 85-93.
[15]	QIN Chengjie, HU Bin, YE Zhenghuai, ZHU Jianfeng, LI Xueling, TIAN Ling, HUANG Zhijun, HU Wenfeng. Effects of Dietary Sodium Chloride Level on Growth Performance and Microbial Community of Black Soldier Fly (Hermetia illucens L.) [J]. Animal Husbandry and Feed Science, 2023, 44(3): 7-17.