布鲁菌疫苗株Rev.1全基因序列的测定与分析

doi:10.12160/j.issn.1672-5190.2020.05.017

Animal Husbandry and Feed Science ›› 2020, Vol. 41 ›› Issue (5): 91-95.doi: 10.12160/j.issn.1672-5190.2020.05.017

Genome-wide Sequencing and Analysis for Brucella Rev.1Vaccine Strain

YU Huan-yu¹, ZHANG Zhi-dan¹, LIU Jian-qi², ZHAO Dan-tong², ZHAO Li-xia², LIU Guo-ying², SONG Qing-qing², WANG Guo-hua³, PAN Xiang-chen³, LIU Dong-dong³, TIAN Pu-hou³, SHI Shun-li⁴, GUAN Ping-yuan¹

1.College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2.National Engineering Laboratory for Veterinary Vaccine, The Spirit Jinyu Biological Pharmaceutical Co., Ltd., Hohhot 010030, China;
3.Inner Mongolia Bigvet Biotech Co., Ltd., Hohhot 011500, China;
4.Livestock Breeding Guide Station of Tongliao City of Inner Mongolia, Tongliao 028000, China

Received:2020-09-08 Published:2020-10-21

Abstract

Abstract: In order to promote the research and development of Brucella Rev.1 vaccine and related vaccines, the nucleic acid of the Brucella Rev.1 vaccine strain was extracted, and the genome-wide sequencing for Brucella Rev.1 vaccine strain was carried out and analyzed through PacBio platform. The results showed that the full-length genome of the Brucella Rev.1 vaccine strain was 3 299 187 bp, and the G+C content was 57.2%; two circular genomes of chromosome 1 and chromosome 2 were assembled; the size of chromosome 1 and chromosome 2 was 2 121 370 bp and 1 177 817 bp, respectively, and the G+C content of chromosome 1 and chromosome 2 was 57.2% and 57.3%, respectively. The sequences of Ery, BLS and VirB10 genes of the Brucella Rev.1 vaccine strain were bioinformatically compared and analyzed with their counterparts of 9 Brucella reference strains, and the sequence similarity among those genes were observed, ranging from 97.4% to 100%.

Key words: Brucella, Brucella Rev.1 vaccine strain, genome-wide sequencing, gene sequence comparison

CLC Number:

S852.614

YU Huan-yu, ZHANG Zhi-dan, LIU Jian-qi, ZHAO Dan-tong, ZHAO Li-xia, LIU Guo-ying, SONG Qing-qing, WANG Guo-hua, PAN Xiang-chen, LIU Dong-dong, TIAN Pu-hou, SHI Shun-li, GUAN Ping-yuan. Genome-wide Sequencing and Analysis for Brucella Rev.1Vaccine Strain[J]. Animal Husbandry and Feed Science, 2020, 41(5): 91-95.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal30.magtechjournal.com/xmysl/EN/10.12160/j.issn.1672-5190.2020.05.017

https://journal30.magtechjournal.com/xmysl/EN/Y2020/V41/I5/91

References

[1] 王树华, 韩一超, 陈剑波.晋北地区奶牛布氏杆菌病发病情况调查及防控建议[J].山西农业科学, 2015, 43(7):888-889, 901.
[2] 王亭. 奶牛布鲁菌病流行病学调查及风险评估模型构建及应用[D].哈尔滨:东北农业大学, 2013.
[3] TAN S Y, DAVIS C.Medicine in stamps david bruce(1855-1931):discoverer of brucellosis[J].Singapore Medical Journal, 2011, 52(3):138-139.
[4] 孙承恩. 布鲁菌病[M].银川:宁夏人民出版社, 1985.
[5] SCHOLZ H C, HUBALEK Z, SEDLÁCEK I, et al. Brucella microti sp.nov, isolated from the common vole Microtus arvahs[J].International Journal of Systematic and Evolutionary Microbiology, 2008, 58(2):375-382.
[6] 于环宇. 布鲁菌Rev.1疫苗株全基因组的序列测定及分析[D].呼和浩特:内蒙古农业大学, 2019.
[7] XIANG Z S, ZHENG W J, HE Y Q.BBP:Brucella genome annotation with literature mining and curation[J].BMC Bioinformatics, 2006, 7:347.
[8] LAPAQUE N, MORIYON L, MORENO E, et al.Brucella lipopolysaccharide acts as a virulence factor[J].Current Opinion in Microbiology, 2005, 8:60-66.
[9] MYENI S, CHILD R, NG T W, et al.Brucella modulates secretory trafficking via multiple type Ⅳ secretion effector proteins[J].PLoS Pathogens, 2013, 9(8):e1003556.
[10] 孙海军. 布氏杆菌病的研究进展[J].当代畜禽养殖业, 2013(7):14-16.
[11] 于薇薇, 李祥, 王春光.布鲁菌病的研究进展[J].养殖技术顾问, 2012(10):216-218.
[12] 张士义, 江森林, 聂志文, 等.国内外防治布鲁菌病主要技术措施的实施及评价[J].中国地方病防治杂志, 2000, 15(1):30-35.
[13] 朱素萍. 人和动物布氏杆菌病疫情时空趋势分析及其相关性研究[D].兰州:西北民族大学, 2013.
[14] 马帅, 李金积, 赵明, 等.布鲁菌病研究进展[J].中国动物检疫, 2020, 37(7):73-79.
[15] GUZMÁN-HERNÁNDEZ R L, CONTRERAS-RODRÍGUZE A, ÁVILA-CALDERÓN E D. Brucellosis: a zoonosis of importance in Mexico[J].Revista Chilena de Infectologia, 2016, 33(6):656-662.
[16] 顾超慧. 羊种布鲁菌强毒株16M与疫苗株M5差异蛋白质组学分析[D].呼和浩特:内蒙古农业大学, 2009.
[17] VELIKOVSKY C A, GOLDBAUM F A, CASSATARO J, et al.Brucella lumazine synthase elicits a mixed Thl-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used[J].Infection and Immunity, 2003, 71(10):5750-5755.
[18] 桂丹, 张辉, 孟仁, 等.布鲁菌ery操纵子突变株生物学特性分析[J].中国生物化学与分子生物学报, 2013, 29(1):76-83.
[19] 程汝佳. 犬种布鲁菌GB1株全基因组测序及其单克隆抗体制备和初步应用[D].泰安:山东农业大学, 2018.
[20] 红梅. 布鲁菌S2疫苗株全基因组测序分析及诊断方法研究[D].呼和浩特:内蒙古农业大学, 2016.
[21] 彭佳. 布鲁菌A19疫苗株全基因组测序与比较基因组学分析[D].南京:南京农业大学, 2016.

Genome-wide Sequencing and Analysis for Brucella Rev.1Vaccine Strain

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	GU Yan-fei, YIN Ying, LI Yu-jie, ZAI Xiao-dong, XU Jun-jie. Preparation Method and Immunogenicity of Recombinant Brucella Outer Membrane Protein Omp19 [J]. Animal Husbandry and Feed Science, 2022, 43(3): 1-7.
[2]	CHENG Jia-hai, SONG Qian-jin, GUO Hao, HOU Li-na, YANG Chao, WANG Feng-xue, GUAN Ping-yuan, WEN Yong-jun. Prokaryotic Expression of eryA Gene in Brucella Rev.1 Strain and Establishment of Indirect ELISA Method [J]. Animal Husbandry and Feed Science, 2020, 41(6): 7-12.
[3]	SHEN Li-ping, WANG Zheng-ting, LIU Zhi-guo. Characterization of the Genotype of Three Clinically Isolated Atypical Brucella Strains from Sheep [J]. Animal Husbandry and Feed Science, 2019, 40(12): 108-112.