冷冻精液输精深度和时间对蒙古羊受胎率的影响

doi:10.12160/j.issn.1672-5190.2021.05.018

Abstract

Abstract: [Objective] To assess the effects of insemination depth and time on conception rate of Mongolian sheep inseminated with frozen semen. [Method] Health Mongolian ewes with normal reproductive capacity were chosen as experimental animals and were subjected to estrus synchronization using progesterone sponges. The ewes were artificially inseminated with frozen semen after estrus using conventional artificial insemination method （n=135）, transcervical insemination method [transcervical first fold （1-2 cm） insemination （n=120）, and transcervical second to third fold （3-4 cm） insemination （n=120）] , and laparoscopically assisted intrauterine insemination method （n=128）, respectively. The recipient ewes receiving conventional artificial insemination and transcervical insemination were separately inseminated once around 14 h and 22 h after estrus, and those receiving laparoscopically assisted intrauterine insemination were inseminated once during 30 h to 36 h after estrus. B-ultrosonic examination was carried out at 40 d after insemination, and the conception rates of the ewes inseminated by different methods were calculated and statistically compared. [Result] The highest average conception rate was observed in the ewes receiving laparoscopically assisted intrauterine insemination （60.2%）, followed by those receiving transcervical second to third fold （3-4 cm） insemination （58.3%）; the average conception rates of the ewes receiving transcervical first fold （1-2 cm） insemination and conventional artificial insemination were 37.5% and 36.3%, respectively. The statistical analysis demonstrated that the average conception rates of the ewes receiving laparoscopically assisted intrauterine insemination and transcervical second to third fold （3-4 cm） insemination were significantly （P<0.05） higher than those of the ewes receiving conventional artificial insemination and transcervical first fold （1-2 cm） insemination. [Conclusion] For Mongolian ewes, laparoscopically assisted intrauterine insemination method （inseminated once during 30 h to 36 h after estrus） that completely penetrates cervix obtains higher conception rate in comparison with conventional artificial insemination method; transcervical insemination method （separately inseminated once around 14 h and 22 h after estrus） with depth of nearing 4 cm achieves similar conception rate to laparoscopically assisted intrauterine insemination method.

Key words: Mongolian sheep, frozen semen, transcervical insemination, laparoscopically assisted intrauterine insemination, conception rate

CLC Number:

S826.34

ZHAO Xia, MA Yue-jun, LIU Bin, WU Tie-cheng, GAO Yu-lin, LI Yu-rong, Wulanqiqige, Suyalatu, Wurentuya. Effects of Insemination Depth and Time on Conception Rate of Mongolian Sheep Inseminated with Frozen Semen[J]. Animal Husbandry and Feed Science, 2021, 42(5): 108-111.

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Effects of Insemination Depth and Time on Conception Rate of Mongolian Sheep Inseminated with Frozen Semen

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