连续重复超数排卵、冲胚对引进品种肉羊快速扩繁的效果研究

doi:10.12160/j.issn.1672-5190.2022.02.011

Abstract

Abstract: [Objective] To assess the applicability of an integrated mode of breeding technologies in mutton sheep of imported breeds, such as continual and repeated superovulation, embryo flushing, and embryo transplantation. [Method] From the year of 2016 to 2018, a total of 1 575 imported mutton ewes belonging to five breeds, including Australian White （n = 230）, White Dorper （n=228）, Dorper （n=541）, White Suffolk （n=200） and Suffolk （n=376）, were treated by continual estrus synchronization （progesterone sponge） and superovulation （330-450 IU FSH + 200-300 IU PMSG + 0.1 mg PG） with an interval of more than 30 days. Intraperitoneal endoscopic insemination was performed to flush out embryos from uterine horn of the ewes. The obtained embryos were counted, evaluated in terms of quality, and then partially transplanted into the synchronized receptors. The impacts of breed, season, and number of repeated superovulation times on embryo productivity were compared and analyzed. [Result] A total of 18 496 embryos were obtained, with 14 971 transferable ones. Gestation rate after embryo transplantation was 69.8% and the lambing rate was 93.2%. According to the mean numbers of recovered total embryos and transferable embryos per ewe, the embryo productivity of White Suffolk, Suffolk and Dorper was superior to Australian White, and White Dorper had the worst performance. After continual and repeated superovulation as well as embryo collection, the mean numbers of recovered total embryos and transferable embryos per ewe of different breeds continued to increase until the 4^th or 5^th treatment. The outcome of superovulation and embryo collection of Australian White was better in spring, while the other breeds were better in winter. [Conclusion] Continual and repeated superovulation as well as embryo collection are feasible breeding technologies for propagation of the mutton sheep of imported breeds. They should be used in a scientific and timely manner, taking into account the various breeds and their seasonal reproductive features.

Key words: continual and repeated superovulation, breeding technologies integration, industrialized application, embryo transplantation

CLC Number:

S826.36

YUE Yong-li, BAO Hua-la, Moderiletu, LI Xue-ling, WANG Jian-guo. Effects of Continual and Repeated Superovulation and Embryo Collection on Rapid Propagation of Mutton Sheep of Imported Breeds[J]. Animal Husbandry and Feed Science, 2022, 43(2): 61-68.

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References

[1] 姜玉柱. 巴美肉羊培育成功[J].农业知识,2008(18):13.
[2] 胡大君,康凤祥.“昭乌达肉羊”新品种的培育[J].中国草食动物科学,2012(S1):244-247.
[3] FORCADA F,AIT AMER-MEZIANE M,ABECIA J A,et al. Repeated superovulation using a simplified FSH/eCG treatment for in vivo embryo production in sheep[J].Theriogenology,2011,75(4):769-776.
[4] GRANLEESE T,CLARK S A,SWAN A A,et al.Increased genetic gains in sheep,beef and dairy breeding programs from using female reproductive technologies combined with optimal contribution selection and genomic breeding values[J].Genetics Selection Evolution,2015,47(1):70.
[5] BARTLEWSKI P M,SEATON P,FRANCO O M E,et al. Intrinsic determinants and predictors of superovulatory yields in sheep:Circulating concentrations of reproductive hormones,ovarian status,and antral follicular blood flow[J]. Theriogenology,2016,86(1):130-143.
[6] 季斐,董红,毋状元,等.体重体尺指标对萨福克羊超排效果的影响[J].草食家畜,2019(3):25-29.
[7] BARI F,KHALID M,WOLF B,et al.The repeatability of superovulatory response and embryo recovery in sheep[J].Theriogenology,2001,56(1):147-155.
[8] 霍飞,罗永明,阿依努尔·亚森,等.规模化胚胎移植肉羊供体羊处理研究[J].中国草食动物科学,2015,35(5):77-79.
[9] KUMAR D,DE K,SHEKHAWAT I,et al.Combined effect of heat and nutritional stress on superovulation of Malpura ewes in a semi-arid region[J].Journal of Thermal Biology,2019,80:158-163.
[10] BRUNO-GALARRAGA M M,CUETO M,GIBBONS A E,et al. Repeatability of superovulatory response to successive FSH treatments in Merino sheep[J].Small Ruminant Research,2014,120(1):84-89.
[11] MENCHACA A,VILARINO M,CRISPO M,et al.New approaches to superovulation and embryo transfer in small ruminants[J].Reproduction,Fertility and Development,2010,22(1):113-118.
[12] AMIRIDIS G S,CSEH S.Assisted reproductive technologies in the reproductive management of small ruminants[J].Animal Reproduction Science,2012,130(3/4):152-161.
[13] BARTLEWSKI P M,ALEXANDER B D,RAWLINGS N C,et al.Ovarian responses,hormonal profiles and embryo yields in anoestrous ewes superovulated with Folltropin-V after pretreatment with medroxyprogesterone acetate-releasing vaginal sponges and a single dose of oestradiol-17beta[J]. Reproduction in Domestic Animals,2008,43(3):299-307.
[14] FIERRO S,VINOLES C,OLIVERA-MUZANTE J.Long term prostaglandin based-protocols improve the reproductive performance after timed artificial insemination in sheep[J].Theriogenology,2017,90:109-113.
[15] 卢小芳,苏布日,王斌,等.不同FSH注射策略对澳洲白绵羊超数排卵效果的分析[J].家畜生态学报,2019,40(5):55-58.
[16] BALARO M F,FONSECA J F,BARBOSA T G,et al.Potential role for GnRH in the synchronization of follicular emergence before the superovulatory Day 0 protocol[J].Domestic Animal Endocrinology,2016,54:10-14.
[17] AZAWI O I,AL-MOLA M K. A study on the effect of GnRH administration on the ovarian response and laparoscopic intrauterine insemination of Awassi ewes treated with eCG to induce superovulation[J].Tropical Animal Health and Production,2011,43(7):1351-1355.
[18] SONG Y K,WU H,WANG X G,et al.Melatonin improves the efficiency of super-ovulation and timed artificial insemination in sheep[J].PeerJ,2019,7:e6750.
[19] TIAN X Z,LV D Y,MA T,et al.AANAT transgenic sheep generated via OPS vitrified-microinjected pronuclear embryos and reproduction efficiency of the transgenic offspring[J].PeerJ,2018,6:e5420.
[20] 张秀陶,许斌,吕建明,等.品种和超排季节对绵羊胚胎移植效果的影响[J].黑龙江畜牧兽医,2006(11):42-43.
[21] 郭延华,万鹏程,刘长彬.肉用绵羊超数排卵效果实例分析[J].新疆农业科学,2017,54(8):1550-1558.
[22] BERGSTEIN-GALAN T G,WEISS R R,KOZICKI L E. Effect of semen and donor factors on multiple ovulation and embryo transfer(MOET)in sheep[J]. Reproduction in Domestic Animals,2019,54(2):401-407.
[23] MILNE C.The history of the Dorper sheep[J]. Small Ruminant Research,2000,36(2):99-102.
[24] 张果平,王金文,王德芹,等.影响杜泊绵羊超数排卵效果的因素分析[J].江苏农业学报,2008,24(3):293-297.
[25] 祖尔东·热合曼,穆塔里甫,阿不来提,等.萨福克羊超数排卵影响因素的研究[J].新疆畜牧业,2011(3):19-23.
[26] 吴姣,王二耀,冯亚杰,等.小尾寒羊重复超排激素用量调整及其效果[J].河南农业科学,2014,43(1):140-143.
[27] 张良. 母羊重复超排对冲胚数的影响[J].当代畜牧,2007(9):41.
[28] 张帆,李晓丽,王俊,等.杜泊绵羊超数排卵及胚胎移植影响因素分析[J].黑龙江动物繁殖,2014,22(3):40-44.
[29] 田秀娥,王永军,付明哲,等.进口和国产激素对绵羊发情和超排效果的研究[J].甘肃畜牧兽医,2018,48(5):4.
[30] 尹启宝,王维群,张星敏,等.萨福克羊不同药物多次超数排卵效果分析[C]//2012中国羊业进展论文集.北京:中国畜牧业协会,2012:3.
[31] 朱芳贤,孟飞,许海涛,等.兰州大尾羊重复超数排卵效果分析[J].中国畜牧业,2014(15):50-51.
[32] 傅文栋,李艳,何成武,等.萨福克肉羊连续超排及胚胎移植研究[J].中国草食动物科学,2017,37(3):72-73.
[33] 奥旭东,陈大勇,石新宇,等.季节对杜泊羊体内胚胎生产与移植效果的影响[J].畜牧与饲料科学,2019,40(1):24-27.
[34] 曾滔,韩煜茹,祁先江,等.萨福克肉羊不同月份超数排卵效果比较[J].草食家畜,2017(6):1-4.
[35] 苟想珍,李刚,贺钰烜,等.陇东地区引进澳洲黑头萨福克羊超数排卵效果的研究[J].中国草食动物科学,2018,38(6):75-77.
[36] GONZALEZ-BULNES A,GARCIA-GARCIA R M,SANTIAGO-MORENO J,et al. Reproductive season affects inhibitory effects from large follicles on the response to superovulatory FSH treatments in ewes[J].Theriogenology,2003,60(2):281-288.
[37] 滑留帅,辛晓玲,王璟,等.利用混池重测序鉴定萨福克绵羊超数排卵关键调控基因[J].畜牧兽医学报,2019,50(9):1813-1821.
[38] MOSSA F,DUFFY P,NAITANA S,et al.Association between numbers of ovarian follicles in the first follicle wave and superovulatory response in ewes[J].Animal Reproduction Science,2007,100(3/4):391-396.
[39] PINTO P,BRAGANCA G M,BALARO M,et al.Colour-Doppler ultrasound imaging as a laparoscopy substitute to count corpora lutea in superovulated sheep[J].Reproduction in Domestic Animals,2018,53(1):266-269.
[40] 赵冰茹,陈童,董红,等.萨福克羊血清激素与超数排卵效果的相关性研究[J].畜牧与兽医,2021,53(8):1-5.
[41] 赵冰茹,陈童,董红,等.萨福克羊血清中蛋白含量与超排效果相关性研究[J].中国畜牧杂志,2021,57(1):120-122.

Effects of Continual and Repeated Superovulation and Embryo Collection on Rapid Propagation of Mutton Sheep of Imported Breeds

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[3]	. Effects of Different Influencing Factors on the Embryo Production Efficiency [J]. Animal Husbandry and Feed Science, 2018, 39(7): 45-45.
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[5]	. Embryo Freezing and Thawing Technology and Its Application in Mutton Sheep Industry in China [J]. Animal Husbandry and Feed Science, 2014, 35(7): 53-53.
[6]	. Study on the Influence Factors of Sheep Embryo Transplantation Pregnancy in the Nonbreeding Season [J]. Animal Husbandry and Feed Science, 2013, 34(9): 23-23.
[7]	. [J]. Animal Husbandry and Feed Science, 2012, 33(5): 120-120.
[8]	. [J]. Animal Husbandry and Feed Science, 2009, 30(3): 74-74.
[9]	. [J]. Animal Husbandry and Feed Science, 2007, 28(3): 26-26.
[10]	. [J]. Animal Husbandry and Feed Science, 2006, 27(5): 42-42.
[11]	. [J]. Animal Husbandry and Feed Science, 2006, 27(4): 50-50.
[12]	. Study on the Super-ovulation of Sheep [J]. Animal Husbandry and Feed Science, 2002, 23(2): 9-9.
[13]	. Use of Embryo Transfer Technique in Breeding of German Mutton Merino [J]. Animal Husbandry and Feed Science, 2001, 22(2): 12-12.
[14]	. [J]. Animal Husbandry and Feed Science, 1999, 20(3): 16-16.
[15]	. [J]. Animal Husbandry and Feed Science, 1995, 16(3): 45-45.