Abstract:

[Objective] To evaluate the protective effects of total flavonoids from Spatholobus suberectus Dunn （TFSD） on liver injury induced by cyclophosphamide （CTX） in mice.[Method] A total of 60 Kunming mice were randomly divided into 1 of 6 groups with 10 mice in each group. The experimental period lasted for 7 days. The control group received physiological saline throughout the whole experiment. The CTX group was gavaged with physiological saline from 1^st to 4^th day of the experiment, and was intraperitoneally injected with 80 mg/（kg·BW） CTX once a day from 5^th to 7^th day of the experiment. TFSD100 group, CTX+TFSD25 group, CTX+TFSD50 group and CTX+TFSD100 group were gavaged with 100, 25, 50 and 100 mg/（kg·BW） TFSD once a day from 1^st to 7^th day of the experiment, respectively. From 5^th to 7^th day of the experiment, all the other experimental groups received intraperitoneal injections of 80 mg/（kg·BW） CTX once a day, with the exception of the TFSD100 group, which received intraperitoneal injections of physiological saline. At the end of the experiment, the mice in each group were sacrificed and their blood samples were taken from the eyeballs. The activities of superoxide dismutase （SOD）, myeloperoxidase （MPO）, xanthine oxidase （XOD）, and glutathione peroxidase （GSH-Px） in liver tissues were tested. In addition, the serum activities of aspartate aminotransferase （AST）, alanine aminotransferase （ALT） and alkaline phosphatase （ALP） as well as the serum levels of tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6） and interleukin-1β （IL-1β） were measured. [Result] CTX significantly （P<0.05） decreased the activities of SOD and GSH-Px in liver tissues and the serum activity of ALT, while significantly （P<0.05） elevated the activities of XOD and MPO in liver tissues, the serum activity of ALP, and the serum levels of IL-1β and IL-6. The treatments of 25, 50 and 100 mg/（kg·BW） TFSD all significantly （P<0.05） inhibited the decrease of SOD activity induced by CTX, and 25 mg/（kg·BW） TFSD significantly （P<0.05） elevated the GSH-Px activity in CTX treated mice. The treatment of 50 mg/(kg·BW) TFSD significantly （P<0.05） inhibited the elevation of XOD and MPO activities induced by CTX, and significantly （P<0.05） reduced the AST activity in CTX treated mice. The treatments of 25, 50 and 100 mg/（kg·BW） TFSD all significantly （P<0.05） inhibited the increase of ALP activity induced by CTX, and reduced the ALT activity. The treatments of 25, 50 and 100 mg/（kg·BW） TFSD all significantly （P<0.05） inhibited the increase of IL-1β level induced by CTX. [Conclusion] TFSD exhibited protective effects on cyclophosphamide induced liver injury by regulating the release of inflammatory factors and the levels of oxidoreductase in liver tissues in mice. The recommended dose of TFSD was 25 mg/（kg·BW）.

Key words: total flavonoids from Spatholobus suberectus Dunn, cyclophosphamide, liver injury, inflammatory factor, oxidoreductase