广西壮族自治区猪圆环病毒2型（PCV2）分子流行病学调查及遗传变异分析

doi:10.12160/j.issn.1672-5190.2024.02.014

Abstract

Abstract: [Objective] This study was conducted to understand the prevalence and genetic characteristics of porcine circovirus type 2 （PCV2） in Guangxi Zhuang Autonomous Region. [Method] The clinical samples collected from different areas of Guangxi Zhuang Autonomous Region were subjected to PCV2 detection and whole genome sequence amplification by PCR assay. The bioinformatics software including BioEdit, Mega 7.0, RDP 5 and SimPlot （ver 3.5.1） were used to perform nucleotide sequence similarity, genetic variation and recombination analyses on the while genome sequences of 24 PCV2 strains obtained. In addition, the variation sites on amino acid sequences of the Cap protein were analyzed. [Result] The genomic size of the PCV2 Guangxi strains were all 1 768 bp. Nucleotide sequence similarity analysis showed that the PCV2 Guangxi strains shared 44.7% to 99.7% similarity with the referential strains PCV1-PCV4, and had the lowest similarity with PCV3. The PCV2 Guangxi strains were determined as PCV2b and PCV2d subtypes, with PCV2d being the most prevalent. Whole genome sequence recombination analysis demonstrated that there were recombination events in selected PCV2 Guangxi strains. In comparison with the vaccine strains AY686764-PCV2b and HM641752-PCV2b, a total of 21 variation sites were observed on the amino acid sequences of the Cap protein of the PCV2 Guangxi strains. [Conclusion] PCV2d was the dominant subtype of the PCV2 Guangxi strains. Several strains had genomic recombination events and unique amino acid variations on some loci, indicating a clear genetic evolution trend. The research results provided basic data for the epidemiological investigation and genetic evolution analysis of PCV2 in Guangxi Zhuang Autonomous Region.

Key words: porcine circovirus type 2, whole genome, similarity, genetic variation

CLC Number:

S852.659.2

HUANG Meizhi, HE Qisong, FENG Shuping, LONG Feng, YIN Yanwen, MO Shenglan, HU Liping, HUANG Shengbin, HAN Yinhua, ZHOU Qing′an, LAN Huihua, WEI Haina, WEI Yuanyuan, GAN Yu, SHI Kaichuang. Molecular Epidemiological Investigation and Genetic Variation Analysis of Porcine Circovirus Type 2 （PCV2）in Guangxi Zhuang Autonomous Region[J]. Animal Husbandry and Feed Science, 2024, 45(2): 109-115.

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URL: https://journal30.magtechjournal.com/xmysl/EN/10.12160/j.issn.1672-5190.2024.02.014

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References

[1] SUH J, OH T, CHAE C.Virulence comparison of 4 porcine circovirus type 2 (PCV-2) genotypes:2a, 2b, 2d, and 2e with a single infection and co-infection with PCV-2 and porcine reproductive and respiratory syndrome virus (PRRSV)[J]. Canadian Journal of Veterinary Research,2022,87(1):41-50.
[2] TING O, XINWEI Z, XIAOHUA L, et al.Co-infection of swine with porcine circovirus type 2 and other swine viruses[J].Viruses,2019,11(2):185.
[3] KARUPPANNAN A K, OPRIESSNIG T.Porcine circovirus type 2 (PCV2) vaccines in the context of current molecular epidemiology[J].Viruses,2017,9(5):99.
[4] POULSEN N B, VAN VLAENDEREN I, MELLENCAMP M A.A chimeric vaccine against porcine circovirus type 2:Meta-analysis of comparative clinical trials[J].Vaccines,2022,11(3):584.
[5] TANJA O, XIANG J M, PATRICK G H.Porcine circovirus type 2-associated disease:Update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies[J].Journal of Veterinary Diagnostic Investigation,2007,19(6):591-615.
[6] LUKA P D, ADEDEJI A J, JAMBOL A R, et al.Coinfections of African swine fever virus, porcine circovirus 2 and 3, and porcine parvovirus 1 in swine in Nigeria[J]. Archives of Virology,2022,167(12):2715-2722.
[7] WEISSENBACHER-LANG C, KRISTEN T, MENDEL V, et al.Porcine circovirus type 2 (PCV2) genotyping in Austrian pigs in the years 2002 to 2017[J]. BMC Veterinary Research,2020,16(1):198.
[8] BAO F, MI S, LUO Q, et al.Retrospective study of porcine circovirus type 2 infection reveals a novel genotype PCV2f[J]. Transboundary and Emerging Diseases,2018,65(2):432-440.
[9] FRANZO G, SEGALÉS J. Porcine circovirus 2 (PCV-2) genotype update and proposal of a new genotyping methodology[J]. PLoS One,2018,13(12):e0208585.
[10] 甘雨,周庆安,许光明,等.广西猪圆环病毒2型及3型的流行病学初步调查[J].广西畜牧兽医,2023,39(6):251-254.
[11] NAN W, WU J, HU H, et al.Prevalence and genetic diversity of porcine circovirus type 2 in northern Guangdong Province during 2016-2021[J]. Frontiers in Veterinary Science,2022,9:932612.
[12] BIAN H, YU C, WEI Y, et al.Purification of porcine circovirus type 2 using an affinity chromatography based on a neutralizing monoclonal antibody against viral capsid protein[J]. Pathogens,2021,10(12):1564.
[13] HUANG Y, CHEN X, LONG Y, et al.Epidemiological analysis from 2018 to 2020 in China and prevention strategy of porcine circovirus type 2[J]. Frontiers in Veterinary Science,2021,8:753297.
[14] WEI C, ZHANG M, CHEN Y, et al.Genetic evolution and phylogenetic analysis of porcine circovirus type 2 infections in southern China from 2011 to 2012[J]. Infection Genetics and Evolution,2013,17:87-92.
[15] QU T, LI R, YAN M, et al.High prevalence of PCV2d in Hunan Province, China:A retrospective analysis of samples collected from 2006 to 2016[J]. Archives of Virology,2018,163(7):1897-1906.
[16] SUN J, HUANG L, WEI Y, et al.Prevalence of emerging porcine parvoviruses and their co-infections with porcine circovirus type 2 in China[J]. Archives of Virology,2015,160(5):1339-1344.
[17] 李玲,李国新,周艳君,等.2008—2011年中国部分地区猪圆环病毒2型的分子流行病学调查[J].中国动物传染病学报,2012,20(2):1-10.
[18] 陈翔鸿,黄英,宋文博,等.2019—2020年我国中南地区猪圆环病毒2型的分子流行病学研究[J].畜牧与兽医,2022,54(11):84-91.
[19] LI N, LIU J, QI J, et al.Genetic diversity and prevalence of porcine circovirus type 2 in China during 2000-2019[J].Frontiers in Veterinary Science,2021,8:788172.
[20] CAI L, NI J, XIA Y, et al.Identification of an emerging recombinant cluster in porcine circovirus type 2[J]. Virus Research,2012,165(1):95-102.
[21] 邓文芳,宋文博,胡星星,等.2017—2018年华中地区猪圆环病毒2型分子流行病学分析[J].中国动物传染病学报,2020,28(2):32-38.
[22] 翟新国,李朋辉,苏金辉,等.河北省及周边地区猪圆环病毒2型的遗传变异与重组分析[J].黑龙江畜牧兽医,2022(5):80-86.
[23] TRIBLE B R, KERRIGAN M, CROSSLAND N, et al.Antibody recognition of porcine circovirus type 2 capsid protein epitopes after vaccination, infection, and disease[J]. Clinical and Vaccine Immunology,2011,18(5):749-757.
[24] HUANG L P, LU Y H, WEI Y W, et al.Identification of one critical amino acid that determines a conformational neutralizing epitope in the capsid protein of porcine circovirus type 2[J]. BMC Microbiology,2011,11: 188.
[25] SAHA D, LEFEBVRE D J, OOMS K, et al.Single amino acid mutations in the capsid switch the neutralization phenotype of porcine circovirus 2[J]. Journal of General Virology,2012,93(7):1548-1555.
[26] KHAYAT R, BRUNN N, SPEIR J A, et al.The 2.3-angstrom structure of porcine circovirus 2[J]. Journal of Virology,2011,85(15):7856-7862.
[27] KANG L, WAHAAB A, SHI K, et al.Molecular epidemic characteristics and genetic evolution of porcine circovirus type 2 (PCV2) in swine herds of Shanghai, China[J].Viruses,2022,14(2):289.

Molecular Epidemiological Investigation and Genetic Variation Analysis of Porcine Circovirus Type 2 （PCV2）in Guangxi Zhuang Autonomous Region

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