生物活性肽的抗氧化作用研究进展

doi:10.12160/j.issn.1672-5190.2026.02.007

Abstract

Abstract: Oxidative stress is an important cause of body damage, decreased immunity and reduced production performance in livestock and poultry. Therefore, the development of green and safe natural antioxidants has become a research hotspot in the healthy breeding of livestock and poultry. Bioactive peptides are a class of small molecule substances with antibacterial, antioxidant, immunomodulatory and other physiological functions. They are widely found in animal and plant proteins and organisms. Among them, antioxidant peptides can alleviate or inhibit oxidative damage in the body. Due to their high activity and multi-target characteristics, antioxidant peptides have good development prospects in the fields of functional food, biomedicine and animal husbandry. This review systematically summarizes the related research results and comprehensively discusses the classification and sources, preparation technologies, antioxidant mechanisms, and activity evaluation methods of bioactive peptides. The material is mainly classified into two categories: plant-derived and animal-derived, with a wide range of raw material sources; the preparation methods mainly include direct extraction, enzymatic hydrolysis, microbial fermentation, chemical synthesis and recombinant DNA technology. Different preparation processes have their own advantages and disadvantages, among which enzymatic hydrolysis and microbial fermentation are more suitable for large-scale production. The antioxidant mechanism mainly includes scavenging free radicals, chelating metal ions, increasing the levels of endogenous antioxidants and regulating antioxidant-related signaling pathways. Its activity can be assessed by chemical evaluation and biological evaluation. In addition, this review discusses the practical application effects of bioactive peptides in livestock and poultry production, analyzes the shortcomings of current research, and provides perspectives on the optimization of preparation process and the realization path of action mechanism, aiming to provide theoretical references for the rational application and in-depth study of bioactive peptides.

Key words: bioactive peptides, oxidative stress, antioxidant peptides, mechanism of action

CLC Number:

S816.7

XU Xiaoyi, GAO Feng. Research Progress on the Antioxidant Effects of Bioactive Peptides[J]. Animal Husbandry and Feed Science, 2026, 47(2): 51-56.

References

[1] ZHANG C Y,YANG M,ERICSSON A C.Antimicrobial peptides:potential application in liver cancer[J].Frontiers in Microbiology,2019,10:1257.
[2] AGERHOLM-LARSEN L,RABEN A,HAULRIK N,et al.Effect of 8 week intake of probiotic milk products on risk factors for cardiovascular diseases[J].European Journal of Clinical Nutrition,2000,54(4):288-297.
[3] ZHU Z Q,XU Z W,LI Y H,et al.Antioxidant function and application of plant-derived peptides[J].Antioxidants,2024,13(10):1203.
[4] CHANG O K,SEOL K H,JEONG S G,et al.Casein hydrolysis by Bifidobacterium longum KACC91563 and antioxidant activities of peptides derived therefrom[J].Journal of Dairy Science,2013,96(9):5544-5555.
[5] SINGH B P,VIJ S.Growth and bioactive peptides production potential of Lactobacillus plantarum strain C2 in soy milk:a LC-MS/MS based revelation for peptides biofunctionality[J].LWT,2017,86:293-301.
[6] LU X,ZHANG L X,SUN Q,et al.Extraction,identification and structure-activity relationship of antioxidant peptides from sesame (Sesamum indicum L.) protein hydrolysate[J].Food Research International,2019,116:707-716.
[7] 霍嘉颖,孙宝国,郑福平,等.白酒中一种三肽Arg-Asn-His的鉴定及其细胞内抗氧化活性[J].食品科学,2018,39(23):126-133.
[8] HE Z H,ZHOU Y,LI S,et al.Bioactive peptides and evaluation of cardiac cytoprotective effects of red millet yellow wine as functional food[J].Foods,2024,13(24):4111.
[9] SOWMYA K,BHAT M I,BAJAJ R K,et al.Buffalo milk casein derived decapeptide (YQEPVLGPVR) having bifunctional anti-inflammatory and antioxidative features under cellular milieu[J].International Journal of Peptide Research and Therapeutics,2019,25(2):623-633.
[10] ABD RASHID N Y,MANAN M A,PA′EE K F,et al.Evaluation of antioxidant and antibacterial activities of fish protein hydrolysate produced from Malaysian fish sausage (Keropok Lekor) by-products by indigenous Lactobacillus casei fermentation[J].Journal of Cleaner Production,2022,347:131303.
[11] LI G S,ZHAN J Q,HU L P,et al.Identification of a new antioxidant peptide from porcine plasma by in vitro digestion and its cytoprotective effect on H₂O₂ induced HepG2 model[J].Journal of Functional Foods,2021,86:104679.
[12] 李思楠,王玺,安宇,等.植物源生物活性肽的制备、生理活性及作用机制研究进展[J].食品工业科技,2025,46(3):394-402.
[13] 任美凤,胡瑞斌,孙芳,等.鸡心SOD的分离纯化及部分性质研究[J].西南大学学报(自然科学版),2013,35(2):35-40.
[14] CRUZ-CASAS D E,AGUILAR C N,ASCACIO-VALDÉS J A,et al.Enzymatic hydrolysis and microbial fermentation:the most favorable biotechnological methods for the release of bioactive peptides[J].Food Chemistry:Molecular Sciences,2021,3:100047.
[15] CHAKRABARTI S,GUHA S,MAJUMDER K.Food-derived bioactive peptides in human health:challenges and opportunities[J].Nutrients,2018,10(11):1738.
[16] AN F Y,CAO K X,JI S Q,et al.Identification,taste characterization,and molecular docking study of a novel microbiota-derived umami peptide[J].Food Chemistry,2023,404:134583.
[17] 谢博,傅红,杨方.生物活性肽的制备、分离纯化、鉴定以及构效关系研究进展[J].食品工业科技,2021,42(5):383-391.
[18] 蒋巧萍. 基于多肽组学的威廉环毛蚓肽类组分分析与活性肽筛选研究[D].北京:北京中医药大学,2024.
[19] 姜明,么越,范蓓,等.食用菌活性成分抗氧化机制的研究进展[J].食品安全质量检测学报,2023,14(9):257-265.
[20] LONG Y Y,TAO H,WANG S Y,et al.Identification and functional validation of two novel antioxidant peptides in saffron[J].Antioxidants,2024,13(3):378.
[21] WANG K,HAN L H,TAN Y Q,et al.Generation of novel antioxidant peptides from silver carp muscle hydrolysate:gastrointestinal digestion stability and transepithelial absorption property[J].Food Chemistry,2023,403:134136.
[22] 张红玉,李会珍,张天伟,等.抗氧化肽作用机制研究进展[J].食品安全质量检测学报,2022,13(12):3981-3988.
[23] 郭洪辉,张怡评,洪专,等.河豚鱼皮胶原寡肽螯合锌的体内体外抗氧化活性研究[J].食品工业科技,2021,42(5):66-71.
[24] ZHENG Y J,WANG X,ZHUANG Y L,et al.Isolation of novel ACE-inhibitory and antioxidant peptides from quinoa bran albumin assisted with an in silico approach:characterization,in vivo antihypertension,and molecular docking[J].Molecules,2019,24(24):4562.
[25] KUMAR N,DEVI S,MADA S B,et al.Anti-apoptotic effect of buffalo milk casein derived bioactive peptide by directing Nrf2 regulation in starving fibroblasts[J].Food Bioscience,2020,35:100566.
[26] WU J N,YI Z W,CHEN X T,et al.Screening and mechanistic study of antioxidant peptides from Bangia fusco-purpurea targeting the Keap1-Nrf2 pathway[J].Food Bioscience,2024,59:104122.
[27] REN L K,FAN J,YANG Y,et al.Identification,in silico selection,and mechanism study of novel antioxidant peptides derived from the rice bran protein hydrolysates[J].Food Chemistry,2023,408:135230.
[28] 孔彩华. 美蠊小肽抗氧化应激诱导的猪卵巢颗粒细胞凋亡的机制研究[D].大理:大理大学,2022.
[29] QI J H,DONG F X.The relevant targets of anti-oxidative stress[J].Journal of Drug Targeting,2021,29(7):677-686.
[30] ZHANG Q Z,TONG X H,LI Y,et al.Purification and characterization of antioxidant peptides from alcalase-hydrolyzed soybean (Glycine max L.) hydrolysate and their cytoprotective effects in human intestinal caco-2 cells[J].Journal of Agricultural and Food Chemistry,2019,67(20):5772-5781.
[31] YU X,CHEN Y N,QI Z G,et al.Preparation and identification of a novel peptide with high antioxidant activity from corn gluten meal[J].Food Chemistry,2023,424:136389.
[32] WIRIYAPHAN C,CHITSOMBOON B,ROYTRAKUL S,et al.Isolation and identification of antioxidative peptides from hydrolysate of threadfin bream surimi processing byproduct[J].Journal of Functional Foods,2013,5(4):1654-1664.
[33] MEMARPOOR-YAZDI M,MAHAKI H,ZARE-ZARDINI H.Antioxidant activity of protein hydrolysates and purified peptides from Zizyphus jujuba fruits[J].Journal of Functional Foods,2013,5(1):62-70.
[34] LÓPEZ-PEDROUSO M,BORRAJO P,AMAROWICZ R,et al.Peptidomic analysis of antioxidant peptides from porcine liver hydrolysates using SWATH-MS[J].Journal of Proteomics,2021,232:104037.
[35] 董丽莎,李妍妍,张红燕,等.黑线鳕鱼皮胶原蛋白胰蛋白酶酶解多肽对UVB诱导HaCaT光损伤的抑制作用[J].食品科学,2018,39(10):185-192.
[36] 杨超. 藜麦活性肽制备及其抗氧化机制研究[D].兰州:甘肃农业大学,2025.
[37] YU X C,LI Z,LIU X R,et al.The antioxidant effects of whey protein peptide on learning and memory improvement in aging mice models[J].Nutrients,2021,13(6):2100.
[38] 张安,廖世永,郭长征,等.大豆酶解蛋白替代豆粕对肉鸡生长性能、屠宰性能、肉品质、血清生化指标和抗氧化能力的影响[J].中国家禽,2025,47(3):71-77.
[39] 胡文举,孙玲利.抗菌肽对固始鸡生长性能、血清抗氧化、免疫功能及肠道微生物的影响[J].饲料研究,2023,46(10):40-43.
[40] 高林,胡海燕,许威,等.重组抗菌肽CC28-纤维素酶对白羽肉鸡生长、肉品质及抗氧化指标的影响[J].中国畜牧杂志,2025,61(12):283-289.
[41] 刘梦雪,刘优优,何云凤,等.日粮中添加抗菌肽对产蛋后期蛋鸡免疫功能和抗氧化能力的影响[J].黑龙江畜牧兽医,2023(6):108-114.
[42] GAD F A,EMAM M A,SHOURBELA R M,et al.The influence of various doses of Moringa oleifera extract on the antioxidant trait,cytokines,reproductive hormones performance,and gonadal histological profiles of Nile tilapia[J].Aquaculture International,2024,32(6):7103-7118.
[43] 王瑛,李泽民,张瑞,等.抗菌肽对荷斯坦公牛生产性能和血清抗氧化能力的影响[J].草业科学,2023,40(10):2682-2692.
[44] LI K L,ZHOU Y Q,TANG Y Q,et al.Deep eutectic solvent-mediated valorization of waste feathers into highly digestible antioxidant peptides for sustainable feed protein[J].Food Chemistry:X,2025,31:103095.

Research Progress on the Antioxidant Effects of Bioactive Peptides

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 8

Recommended Articles

Metrics

Comments

[1]	XIN Liang, WANG Dezhi, YIN Wen, LI Cheng, LI Cuiting, XUAN Zhiwen, MA Yanfen. Research Progress on the Application of Microecological Preparations in Calf Growth and Development [J]. Animal Husbandry and Feed Science, 2026, 47(2): 36-41.
[2]	HUANG Yuzhi, HE Hang, HUANG Yulu, XU Lanmeng, YAN Qing, SU Shaofeng, ZHANG Jie. Mechanisms and Application Strategies of Gut Microbiota-Induced Muscle Metabolic Abnormalities in Livestock and Poultry via the Gut-Muscle Axis [J]. Animal Husbandry and Feed Science, 2026, 47(1): 1-8.
[3]	YIN Kun, TIAN Kaiyue, LIANG Lu, ZHANG Donghui, HUANG Yubo. Research Progress on the Mechanisms of Action and Influencing Factors of Commonly Used Chemical Disinfectants in Livestock and Poultry [J]. Animal Husbandry and Feed Science, 2025, 46(4): 102-109.
[4]	GAO Tian, DU Donghua, Hasisurong . Research Progress in Protective Effects of Camel Milk on Different Types of Liver Injury [J]. Animal Husbandry and Feed Science, 2023, 44(4): 116-121.
[5]	FANG Zhou, MA Xue-hu, AN Yan-hao, SUN Yi-shuo, DOU Wen-li, MA Yan-fen. Oxidative Stress and Inflammation in Livestock and Poultry and Intervention Effects of Polyphenols [J]. Animal Husbandry and Feed Science, 2022, 43(2): 14-22.
[6]	ZHANG Xin-zhuang, CAO Di, Gerelchimeg, Manglai. Research Advances on Molecular Regulatory Mechanisms of Oxidative Stress and Nutritional Strategies in Livestock and Poultry Production [J]. Animal Husbandry and Feed Science, 2021, 42(4): 29-36.
[7]	Baohua, SONG Li-wen, ZHANG Chun-hua, YI Jing, MA Yan-fen. Research Progress on Oxidative Stress and Antioxidant Defense System and Their Relationship with Inflammatory Response in Perinatal Dairy Cows [J]. Animal Husbandry and Feed Science, 2019, 40(6): 53-57.
[8]	. Role of Nrf2-ARE Pathway in Oxidative Stress Injury and Its Relationship with Other Signaling Pathways [J]. Animal Husbandry and Feed Science, 2016, 37(3): 42-42.