Abstract: The SDS-polyacrylamide gel electrophoresis was taken to the rapid detection research on milk quality. Through the exploration on conditions such as dilution multiple, voltage conditions, dyeing time, washing time and times, and the milk protein electrophore-togram with clear bands was obtained. The test result showed that, the optimum condition of milk protein identification with rapid polyacrylamide gel electrophoresis was that 8 % separation gel was used for electrophoresis under 120 V and the protein electrophoresis dyeing solution with super sensitivity was taken to rapidly stain, with boiling for 1 min and shaking for 10 min, which could realize the aim of rapid identification of milk protein.