Animal Husbandry and Feed Science ›› 2009, Vol. 30 ›› Issue (3): 51-51.doi: 10.12160/j.issn.1672-5190.2009.03.023

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Determination on Benzoic Acid in Feed by SPE-HPLC

SUN Shu-hong, JIANG Ling-ling, ZHAO Yan-li (Anshan Product Quality Supervision and Testing Institute, Anshan 114001 ,China)   

  • Online:2009-03-20 Published:2009-03-20

Abstract: The benzoic acid in feed was determined by reversed-phase high performance liquid chromatography (RP-HPLC) and the determination method was established. Benzoic acid in feed was extracted by methanol, purified by C18 solid phase extraction column and detected by RP-HPLC. The linear relationship of benzoic acid in the range of 50-250 g/L was better and the limit of detection of benzoic acid in feed was 0.006 g/kg.

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