Abstract: In this study, an lateral flow strip for the determination of Furahadone Metabolin（FM-AOZ）was made. A monoclonal antibody against （FM-AOZ） was covalcntly conjugated with fluorescent nanoparticle, and was employed in the development of lateral flow strip assay. Crossing-reaction could be emerged between the prepared strip and FM-AOZ, but it didn＇t react to others. It could satisfy the need of detection with1.0 ng/mL of its sensitivity, meanwhile it also could be used semi-quantitative test of FM-AOZ through observing the brightness between detection line and quality control line with eye. The conclusion was made that FM-AOZ from foods can be detected by it.