Abstract: High purity ovotransferrin was extracted from albumen by precipitation with ammonium sulfate and ion exchange chromatography in the study. Albumen was treated with dilution,microfiltration and ultrafiltration to remove mucoprotein in advance,then the tests on quantitatively salting-out was made to ensure the optimal condition by using different saturated degrees of sodium ammonium sulfate,the coarse egg albumen was obtained by precipitation with 60 % saturated degrees of sodium ammonium sulfate. The results showed that the purity of the isolated ovotransferrin reached 95 % from the coarse egg albumen by DEAE-sepharose F. F,by detection of SDS-PAGE,its molecular weight was determined by solvent resistant column and its molecular weight was 76 000. It played the foundation for continuous extraction technology from egg albumen.