Abstract: To mastered the situation of dogs and cats infected with Toxoplasma in Hohhot,ELISA and GICA were applied to detect antibody titer of Toxoplasma among 254 cats and dogs,and the antigen of Toxoplasma was detected by GICA.The result indicated that the positive rate of Ab in dogs and cats respectively was 12.6% and 10.3% by detecting of ELISA,the positive rate of Ab in dog and cat respectively was 11.4% and 9.2% by GICA,the positive rate of Toxoplasma was 23.8% and 33.3% by GICA respectively.The survey showed that there were markly different between different sources of cats and dogs in infection rate（P0.01）,therefor,it is essential to strengthen the management of randomly rearing and stray cats,meanwhile it should strengthen serological monitoring to prevent it effectively in time.