Abstract: [Objective]The research aimed to study the effects of organophosphorus pesticide on the growth and physiological function of Bacillus subtilis and analyze the growth and morphological characteristics changes of B. subtilis isolated from the soil around parterre under the action of typical organophosphorus pesticide DDVP. [Method]Several media with different concentrations of pesticide were set to determine its absorption value at the wavelength of 600 nm. And the growth curve was drawn. [Result] The results showed that 20 and 50 mg/L organophosphorus pesticide promoted the growth of B. subtilis and the promotion of 20 mg/L organophosphorus pesticide was more obvious. These could provide basis for further study on the degradation of organophosphorus pesticide by B. subtilis and the prediction of its action process.