Abstract: [Objective] To establish the cultivation method of bovine endometrial tissue in vitro and to detect the expression of the receptors of PGE_2 and PGF_（2α）in the cultured endometrium. [Methods] The fresh endometrial tissue samples from bilateral uterine horn of health dairy cows in proestrus were collected and used for suspension cultivation in vitro. The cultured endometrium was histologically identified, and the expression of the receptors of PGE_2, including EP1, EP2, EP3（EP3A、EP3B、EP3C、EP3D）, EP4,and the receptors of PGE_2αFP were evaluated by RT-qPCR. [Results] The histological assessment indicated that the structure of cultured bovine endometrium in vitro was intact, and the morphology of the glands and nuclear of the cells was normal. The expression of all the tested receptors of PGE_2 and PGF_（2α）were found in the cultured samples from bilateral uterine horn of dairy cows in proestrus. The expression of the receptors varied, the highest relative expression was observed in EP4, and the expression of EP1, EP3 B and FP was relatively lower. [Conclusion] The in vitro cultivation method of bovine endometrial tissue was successfully established. The expression of the receptors of PGE_2 and PGF_（2α）were found in cultured endometrium, and the relative expression of different types of receptors varied.