Abstract: This study aimed to assess the effect of Cistanche tubulosa ethanol extract on blood biochemical parameters and cardiovascular function in rats and to evidence the assistant therapy action of Cistanche tubulosa on cardiovascular insufficiency from the aspect of modern medicine. A total of 24 rats were randomly assigned to 4 groups. The low, moderate and high dosage groups were administered intragastrically 0.1, 0.2 and 0.3 g/（100 g·BW） of Cistanche tubulosa ethanol extract, and the control group was administered intragastrically the same volume of normal saline. The experimental period lasted for 30 days. At the end of the experiment, content of the main blood biochemical parameters, activity or content of the enzyme and hormone associated with cardiovascular function, organ coefficient, and the motility parameters of carotid artery smooth muscle of rats in treatment groups and control group were determined and statistically evaluated. The results showed that the low dosage of Cistanche tubulosa ethanol extract significantly increased the serum ALT content（P〈0.05）, but no significant differences in the other blood biochemistry parameters between the 3 treatment groups and control group were observed（P〈0.05）; The 3 levels of Cistanche tubulosa ethanol extract had no significant effect on activity of the 3 kind of myocardial enzymes; The Ang-Ⅱ content of low and moderate dosage group was significantly lower than that of control group（P〈0.05）, but no significant effect on EPO activity and ALD content was observed（P〈0.05）; No significant effect of 3 levels of Cistanche tubulosa ethanol extract on growth rate and organ coefficient was observed（P〈0.05）; The motility parameters of carotid artery smooth muscle exhibit a decreasing tendency（P 0.05）. The combined data suggests that Cistanche tubulosa ethanol extract has a minor effect on the blood biochemical parameters and cardiovascular function in rats.