Abstract: [Objective] To reveal the sequence characteristics of cinnamyl alcohol dehydrogenase gene （HdCAD） in Hordeum distichon. [Method] A pair of specific primers designed by referring HdCAD in transcriptome data of Hordeum distichon were used to clone HdCAD gene by RT-PCR. The gene sequence of HdCAD and HdCAD protein sequence encoded by this gene were bioinformatically analyzed. [Result] The total length of the coding region of HdCAD gene was 1 071 bp, encoding 356 amino acids in total. The HdCAD protein contained 17 phosphorylation sites, and the predicted location was in cytoplasm. There were no transmembrane domains and signal peptide sites in HdCAD protein. In the secondary structure, random coiled elements accounted for the highest proportion. The HdCAD protein contained a typical CAD1 domain which belonged to the MDR superfamily. The phylogenetic tree demonstrated that HdCAD protein was closely related to Triticum dicoccum, Aegilops tauschii and Tibet naked barley.[Conclusion] The HdCAD gene is successfully cloned, and the sequence characteristics and functions of its encoding protein are predicted by bioinformatics analysis, which provides a basis for researches on resistance to abiotic stress of Hordeum distichon.

Key words: Hordeum distichon, cinnamyl alcohol dehydrogenase, gene cloning, bioinformatics analysis