[Objective] To establish immortalized ovine ruminal fibroblast cell line and to provide stable in vitro cell model for basic research and animal virus research. [Method] Ovine ruminal fibroblast cells（ORFCs） were cultured by using tissue fragment clinging method. pCI-neo-hTERT plasmid with hTERT gene was transferred into ORFCs using PEI reagents. The positive clone cells were screened by G418. The transcription and expression level of hTERT gene in 15^th and 35^th generation positive clone cells were detected by RT-PCR and Western Blot. The growth curves of primary cells and 35^th generation positive clone cells were drawn with blood cell count method to assess the proliferation ability. [Result] The hTERT gene was successfully transferred into ORFCs and stably expressed. The proliferation ability of positive clone cells was significantly higher than that of primary ORFCs. [Conclusion] Immortalized ovine ruminal fibroblast cell line is successfully established.

[Objective] To reveal the sequence characteristics of cinnamyl alcohol dehydrogenase gene （HdCAD） in Hordeum distichon. [Method] A pair of specific primers designed by referring HdCAD in transcriptome data of Hordeum distichon were used to clone HdCAD gene by RT-PCR. The gene sequence of HdCAD and HdCAD protein sequence encoded by this gene were bioinformatically analyzed. [Result] The total length of the coding region of HdCAD gene was 1 071 bp, encoding 356 amino acids in total. The HdCAD protein contained 17 phosphorylation sites, and the predicted location was in cytoplasm. There were no transmembrane domains and signal peptide sites in HdCAD protein. In the secondary structure, random coiled elements accounted for the highest proportion. The HdCAD protein contained a typical CAD1 domain which belonged to the MDR superfamily. The phylogenetic tree demonstrated that HdCAD protein was closely related to Triticum dicoccum, Aegilops tauschii and Tibet naked barley.[Conclusion] The HdCAD gene is successfully cloned, and the sequence characteristics and functions of its encoding protein are predicted by bioinformatics analysis, which provides a basis for researches on resistance to abiotic stress of Hordeum distichon.

[Objective] To assess the effects of adding Caragana korshinskii in pelleted diet on growth performance, blood biochemical indicators, ruminal fermentation and mutton quality of Tan sheep. [Method] A total of 20 healthy castrated Tan sheep with similar weights were selected and randomly divided into 2 groups with 10 individuals for each group. The control group was fed with pelleted diet containing 10% chrysanthemum meal, and the experimental group was fed with pelleted diet containing 6% Caragana korshinskii and 4% chrysanthemum meal instead. A 50-day fattening experiment was conducted, and the growth performance indicators of the Tan sheep were determined; after the fattening experiment, the blood biochemical indexes were measured; the slaughter performance indicators were evaluated, and the ruminal fluid samples were collected after slaughter to assess the ruminal fermentation indicators; the contents of crude fat, crude protein and amino acids as well as the contents and composition of fatty acids in longissimus dorsi muscle were determined. [Result] No significant （P>0.05） differences in initial weights and final weights in the fattening experiment between the experimental group and the control group were observed; while the average daily gain of the experimental group was reduced by 10.84% compared with the control group, no significant difference was found （P>0.05）; the average daily feed intake of the control group was significantly （P<0.05） higher than that of the experimental group; the two groups had the same feed to gain ratio. The experimental group had significantly （P<0.05） higher serum total protein concentration compared with the control group, and there were no significant （P>0.05） differences in the other tested blood biochemical indexes. The slaughter rate of the control group was 1.59% higher than that of the experimental group, but no significant （P>0.05） difference was observed; there were no significant （P>0.05） differences in liver weights, liver indexes, heart and lung weights and heart and lung indexes between the experimental group and the control group. The experimental group had significantly （P<0.05） higher butyric acid concentration and significantly （P<0.05） lower isovaleric acid concentration in ruminal fluid compared with the control group. A total of 18 and 20 kinds of fatty acids were determined in longissimus dorsi muscle of the control group and the experimental group, respectively. The contents of crude protein, crude fat and total amino acids of the control group and the experimental group were 17.51% and 20.92%, 3.22% and 3.89%, and 13.25% and 14.09%, respectively. [Conclusion] Addition of Caragana korshinskii in pelleted diet has no obvious impacts on most of the indicators associated with growth performance, blood biochemistry and slaughter performance of Tan sheep, but improves the ruminal fermentation and enriches the fatty acids composition and concentrations as well as increases the contents of crude protein, crude fat and total amino acids in mutton.

[Objective] To assess the short-term fattening effect and slaughter performance of Inner Mongolia Arbas white cashmere goat kids. [Method] A total of 520 four-month-old weaned goat kids with similar weights were selected. Among them, 260 kids （experimental group） were fattened under whole confined farming mode in 3 separate herdsmen′s families with the same feeding management standards and feed supply, and the remaining 260 kids （control group） were raised under traditional grazing mode in 2 separate herdsmen′s families with similar pasture quality. The fattening period lasted for 4 months. The weights and average daily gains at different fattening stages under different feeding modes were measured, calculated and statistically compared; a slaughter test was carried out at the end of the fattening experiment to assess the effect of different feeding modes on slaughter performance indicators of Arbas white cashmere goats. The economic benefits of different feeding modes were calculated and compared. [Result] Compared with the control group, the weights on 120^th day of fattening experiment of the experimental group were significantly （P<0.05） increased; the average daily gains during 30 to 60 days and 60 to 90 days of the experimental group were significantly （P<0.05） increased, and those during 90 to 120 days of the experimental group were extremely significantly （P<0.01） increased. The live weights before slaughter, carcass weights, slaughter rates, net meat weights, net meat rates, bone weights, kidney and kidney fat weights, and eye muscle areas of the experimental group were extremely significantly （P<0.01） higher than those of the control group, while the bone-meat ratio of the experimental group was extremely significantly （P<0.01） lower than that of the control group. Non-carcass components such as the blood weights, head and hoof weights, tare weights, digestive system weights （stomach and intestine）, visceral weights （heart, liver, lung）, and omental fat and mesentery fat weights of the experimental group were extremely significantly （P<0.01） higher than those of the control group. The fattening net profit of the experimental group was 345.60 yuan/head higher than that of the control group.[Conclusion] Compared with the traditional grazing mode, the weight gain and slaughter performance of Albas white cashmere goats fattened under whole confined farming mode are significantly improved, and good fattening economic benefits are achieved. The whole confined fattening mode is suitable for promotion in Ordos City.

[Objective] To assess the addition of molasses and Lactobacillus plantarum on fermentation quality of yellow millet straw silage. [Method] Two species of millet straw, NO. 106 and NO. 107 grown on the experimental field of Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, were used as materials. The experimental group added molasses （fresh weight 1.0%） and Lactobacillus plantarum（10⁶ CFU/g fresh weight） to prepare the yellow millet straw silage, and the control group received no addition. There were 3 replicates in each group. The millet straw was fermented at 10-15 ℃ for 90 days. After opening, the pH value, nutrient contents, concentrations of lactic acid and volatile fatty acid, and the ratio of lactic acid to acetic acid of the yellow millet straw silage were determined. [Result] The addition of molasses and Lactobacillus plantarum extremely significantly （P<0.01） elevated the concentrations of lactic acid and acetic acid as well as the ratio of lactic acid to acetic acid of the yellow silage prepared with NO. 106 and NO. 107 millet straw. The ADF content of the yellow silage prepared with NO. 106 millet straw was significantly （P<0.05） decreased by adding molasses and Lactobacillus plantarum, and the pH value of the yellow silage prepared with NO. 107 millet straw was extremely significantly （P<0.01） reduced by adding molasses and Lactobacillus plantarum. [Conclusion] Addition of molasses and Lactobacillus plantarum improves the fermentation quality of yellow millet straw silage.

Long-term use of antibiotics in animals leads to declined immunity and causes endogenous infections and double infections in livestock and poultry. Therefore, the development of green, high-efficient and safe new feed antibiotic substitutes （ASs） has become a hot spot in the development of current feed industry. There are many types of ASs, including acidulants, antibacterial peptides, Chinese herbal medicines, microecological preparations and other products, and they are considered safe, environmentally friendly and effective. This article reviews the application of acidulants, γ-aminobutyric acid, hydrolyzed tannic acid, plant extracts, antimicrobial peptides, enzyme preparations, probiotics, Chinese herbal medicines, etc. in pig production, in hoping to provide references for the development of ASs.

Antibiotics are used as the main feed additives in livestock and poultry. However, their extensive applications over the past decades have resulted in a series of problems which are needed urgently to be solved. For example, antimicrobial resistance of pathogenic bacteria has been enhanced, animal immunity and animal vaccine titer have been reduced, and human health has been affected through food chain. China′ s Ministry of Agriculture and Rural Affairs had issued a notice that the use of antibiotics additives should be banned in China′ s feed industry since the year of 2020, and the feed industry has officially entered an antibiotic-free era. For a long time, researchers have been actively seeking to develop various antibiotics substitutes as well as green and harmless feed additives to eliminate or mitigate the influences brought by prohibition of using antibiotics. In this paper, the antibacterial characteristics and mechanisms of the main antibiotics substitutes are reviewed, in hoping to provide references for the application of antibacterial additives in feed after the ban of antibiotics.

The previous studies demonstrate that the main functions of vitamin D （VD） are to promote bone growth and prevent rickets by regulating Ca ions. However, in recent years, it has been found that VD not only plays an important role in skeletal diseases, but also is closely associated with a variety of extra-skeletal diseases, including cardiovascular diseases, metabolic disorders, malignant tumors, allergic diseases, autoimmune diseases, reproductive diseases, etc. At present, although the researches on the biological functions of VD have become more and more comprehensive and in-depth, most of the them focus on humans and mice. There is limited information on extra-skeletal biological function of VD in different animals, such as cattle, pigs, dogs, cats and so on. Therefore, on the basis of consulting related literatures, this article summarizes the research advances of biological functions of VD on blood pressure, immune regulation, endocrine, endometrial and skeletal muscle, and so on, in hoping to provide a better understanding of biological functions of VD in extra-skeletal system of different animals.

Mycotoxin contamination is an important factor that affects the animal feed safety. Ochratoxin A （OTA） is widely distributed in feed raw materials for broiler chickens. Numerous studies have shown that OTA has nephrotoxicity, hepatotoxicity, cytotoxicity, genetic toxicity, carcinogenicity, teratogenicity, immunotoxicity and intestinal toxicity on broiler chickens, which not only brings great economic losses to broiler chickens rearing industry, but also poses a threat to animal and human health. In this paper, the research advances of OTA in China and abroad are summarized but reviewed, and its toxic mechanisms on broiler chickens are expounded from the aspects of intestinal toxicity, hepatotoxicity, nephrotoxicity and immunotoxicity induced by OTA, in hoping to provide references for further study of OTA.

[Objective] To characterize the growth and development laws of grazing Yunling goat kids during lactation period. [Method] A total of 36 Yunling goat kids including 17 male kids and 19 female kids produced by 24 nanny goats were chosen. During the 0 to 8 weeks of age, the kids were weighted once a week and the body size traits were measured once every other week. The growth curve fitting analysis on weights of male and female kids aged 0 to 8 weeks was conducted using three nonlinear models of Logistic, Gompertz and Von Bertalanffy, and the correlation analysis was carried out between birth weights and weights at the end of the experiment.[Result] The three used models all well fitted the growth curve of Yunling goat kids during lactation period with fitting degree greater than 0.99. The Gompertz and Von Bertalanffy models had better fitting performance. The inflection point times of the growth and development curve of male kids were 3.212 weeks and 2.862 weeks, and the weights at the inflection point were 5.691 kg and 5.352 kg, respectively; the inflection point times of that of female kids were 1.927 weeks and 1.325 weeks, and the weights at the inflection point were 4.532 kg and 3.960 kg, respectively. There were differences in weeks of age and weights in inflection point between male and female kids. The weight growth rate of male kids peaked at 3.037 weeks of age averagely, while that of female kids peaked at 1.626 weeks of age averagely. Birth weights of the kids had significant （P<0.01） effect on weights at the end of the experiment. [Conclusion] The results of this study provide references for scientific rearing of grazing Yunling goats and rational supplementary feeding of Yunling goat kids during lactation period.

[Objective] To assess the effects of human chorionic gonadotropin （hCG） on follicular development, ovulation rate, pregnancy rate and serum reproductive hormone level in jenny.[Method] A total of 60 jennies with dominant follicle diameters of 30-35 mm （n=30） and larger than 35 mm （n=30） were selected. For either of the two jenny herds with different dominant follicle diameters, a 500 IU/head hCG treatment group （n=10）, a 1 000 IU/head hCG treatment group （n=10）, and a control group （n=10） without hCG treatment, were set respectively. The experimental jennies received hCG treatment by intramuscular injection. B-ultrasound examination were carried out every 24 h to observe the follicular development status, and the follicle diameters were determined. The heads of ovulated jennies were recorded, and the ovulation rate was calculated. Artificial insemination was performed. Pregnant examination was carried out on 18^th day after insemination. The heads of pregnant jennies were recorded, and the pregnancy rate was calculated. The serum levels of estradiol （E₂） and progesterone （PROG） were measured at 0, 24, 48 and 72 h after hCG treatment. [Result] In both of the jenny herds, the follicle diameters were increased with the elevation of hCG dosage; for the jennies with dominant follicle diameters larger than 35 mm, the two treatment groups ovulated within 24 h after receiving intramuscular injection of hCG, while no ovulation was observed in the control group; in both of the jenny herds, the ovulated heads and ovulation rate of the treatment groups were increased compared with their corresponding control groups at 48 h after hCG treatment; for the jennies with dominant follicle diameters larger than 35 mm, the ovulation rate reached up to 100% at 72 h after 1 000 IU/head hCG treatment; in both of the jenny herds, the conception rates of the hCG treated jennies were higher than those of the controls, and showed an increasing trend with the elevation of hCG dosage. For the jennies with dominant follicle diameters larger than 35 mm, the pregnancy rate reached up to 50% at 72 h after 1 000 IU/head hCG treatment. In both of the jenny herds, the serum E₂ levels were largely increased at 24 h after 1 000 IU/head hCG treatment compared with that at 0 h, and the serum PROG levels also exhibited an overall increasement during 0 to 72 h. [Conclusion] Intramuscular injection of hCG improves the ovulation rate, pregnancy rate and serum levels of E₂ and PROG of jenny, and the dosage of 1 000 IU/head has better efficacy.

The breed improvement of sports horses has promoted the cultivation and development of modern horse breeds. In modern horse training, the harmonious cooperation of the trainer and rider with horse is very important, and it is the technical guarantee for the excellent equestrian performance and achieving outstanding score. This paper reviews the research advances on testing methods and related candidate genes of horse personality behavior, in hoping to provide references for vigorous development of the modern horse industry.

Mongolian sheep is the most numerous and widely distributed coarse-haired sheep breed in China. It has strong physique, high adaptability, and superior tolerance to extremely extensive feeding and management conditions. The strong ability of tail fat deposition makes it a good resistance to adversity. However, excessive tail fat deposition is not good for meeting the consumer′s demand. Combining the existing data and research results, this review summarizes the origin of Mongolian sheep and the regulation mechanism of tail fat deposition, in hoping to lay a scientific foundation for understanding the origin and clarifying the breeding goals of Mongolian sheep.

[Objective] To assess the characteristics and variation of spatial heterogeneity of Stipa breviflora as constructive species in desert steppe under different grazing intensities. [Method] The constructive species of Stipa breviflora was taken as the research object. The semi-variance function, Kriging interpolation and multi-fractal dimension were used to assess its spatial heterogeneity.[Result] Under different grazing intensities, the mean population density of Stipa breviflora was heavy-grazing area （HG） > no-grazing area （CK） > moderate-grazing area （MG）; Stipa breviflora population had multi-fractal characteristics; the spatial distribution of Stipa breviflora varied under different grazing intensities; the structure ratio （C/C₀+C） of CK was the largest, followed by HG, and both of them were greater than 75%, indicating that CK and HG had strong spatial autocorrelation; the structure ratio （C/C₀+C） of MG was the lowest, which ranged from 25% to 75%, indicating that MG had moderate spatial autocorrelation; structural factors were the main causes of spatial variation of Stipa breviflora.[Conclusion] Heavy-grazing increases the population density of Stipa breviflora, while moderate-grazing decreases the population density of Stipa breviflora due to the low-intensity feeding and trampling from livestock; under different grazing intensities, structural factors mainly contribute to the spatial heterogeneity of Stipa breviflora; the Stipa breviflora population in CK has the most complex spatial distribution and strong spatial heterogeneity.

[Objective] To assess the impacts of different combinations of restoration measures on plant community characteristics of desert steppe, and to analyze the economic benefits produced by different restoration measures. [Method] Five different combinations of restoration measures, including enclosure + grazing prohibition （T1）, enclosure + grazing prohibition + application of organic fertilizer （T2）, enclosure + grazing prohibition + application of compound fertilizer （T3）, enclosure + grazing prohibition + application of compound fertilizer + application of organic fertilizer + reseeding （T4）, and enclosure + grazing prohibition + application of compound fertilizer + reseeding （T5）, were used to restore the degraded desert steppe areas with free-grazing area（T6） as control in Hulestai Sumu, Urad Middle Banner, Bayannur City. Different combinations of restoration measures were conducted in June 2020, and the plant community characteristics in different restoration areas and free-grazing area were investigated in late August of the same year. Furthermore, the economic benefits produced by different restoration measures were calculated and analyzed. [Result] The growth rates of height, coverage, density and above-ground biomass of plant community in the 5 restoration areas were all significantly （P<0.05） higher than those of the control area; T5 area had the highest height growth rate which was significantly （P<0.05） higher than that of T1,T2,T3 and T4 areas; the highest coverage growth rate was observed in T5 area, which was significantly （P<0.05） higher than that of T1 and T3 areas; T4 area had the highest density growth rate which was significantly （P<0.05） higher than that of T1 and T3 areas; T5 area had the best performance in above-ground biomass, which was significantly （P<0.05） higher than that of T1 area. Referencing with the free-grazing area （0 Yuan/hm²）, the highest current-year net profit was obtained in T3 area, which reached up to 9 944 Yuan/hm²; while T4 area produced the lowest current-year net profit, which was 6 601 Yuan/hm². [Conclusion] The restoration combination of enclosure + grazing prohibition + application of compound fertilizer + reseeding has the best performance in ecological restoration, and that of enclosure + grazing prohibition + application of compound fertilizer has the highest current-year net profit. Enclosure + grazing prohibition is the premise to ensure the efficacy of restoration and improvement in degraded desert steppe.

[Objective] To investigate the effects of combinative application of nitrogen （N） and phosphorus （P） fertilizers based on improvement by root cutting on soil nutrient of Leymus chinensis mowing meadow in Horqin grassland. [Method] On the basis of root cutting, a field experiment of adding N and P fertilizers at different concentrations was conducted in the Leymus chinensis mowing meadow. Using a randomized block design, four treatment levels of N and P fertilizers, including CK （control）, NP1 （urea 75 kg/hm²+diammonium phosphate 45 kg/hm²）, NP2 （urea 150 kg/hm²+diammonium phosphate 90 kg/hm²）, and NP3 （urea 225 kg/hm²+diammonium phosphate 135 kg/hm²), were set. There were 3 blocks as repeats for each treatment, and there was a total of 12 experimental plots. In mid-August 2014, a 1 m × 1 m quadrat was randomly set in each plot, and the soil samples were collected after cutting the plant samples at 0 cm stubble; three sampling points were randomly chosen, and the contents of soil N, P and potassium （K） as well as soil organic matter were determined by Kjeldahl method, alkaline hydrolysis method, molybdenum-antimony anti-colorimetric method, acid dissolution-molybdenum-antimony anti-colorimetric method, sodium hydroxide fusion method, ammonium acetate extraction-flame photometric method, and potassium dichromate titrimetric method.[Result] Compared with CK, NP3 treatment induced the significant （P<0.05） reduction of total N content in 10-20 cm soil layer; the 3 fertilization treatments significantly （P<0.05） increased the available N content in 0-10 cm and 10-20 cm soil layers; NP3 treatment significantly （P<0.05） increased the contents of total P and available P in 0-10 cm soil layer, and NP2 treatment significantly （P<0.05） increased the available P contents in 0-10 cm and 20-30 cm soil layers; NP2 and NP3 treatments induced the significant （P<0.05） reduction of total K contents in 10-20 cm and 20-30 cm soil layers, and NP3 treatment induced the significant （P<0.05） reduction of available K content in 0-10 cm soil layer; the 3 fertilization treatments significantly （P<0.05） increased the organic matter contents in different soil layers.[Conclusion] Considering the changes in soil nutrient contents after applying N and P fertilizers, a combinative application of K fertilizer at a certain level is necessary to improve the soil nutrient status of the Leymus chinensis mowing meadow in Horqin grassland.

[Objective] To improve the quality standard of Chuanmei Sanhuang powder. [Method] Microscopic identification method was used to perform the microscopic characteristics identification of Rheum palmatum, Scutellaria baicalensis, Phellodendron amurense, Andrographis paniculate and Armeniaca mume. Thin-layer chromatography （TLC） was employed for qualitative identification of Rheum palmatum and Phellodendron amurense. [Result] Rheum palmatum, Scutellaria baicalensis, Phellodendron amurense, Andrographis paniculate and Armeniaca mume had obvious microscopic characteristics. High accuracy and good repeatability of Rheum palmatum in TLC assay was observed, while Phellodendron amurense had unsatisfactory performance. [Conclusion] The established identification method is simple, and has a good repeatability, which can be used to improve the quality standard of Chuanmei Sanhuang powder.

[Objective] To assess the physical-chemical quality of different parts of Simmental raw beef, and to evaluate the edible quality and sensory quality of the roasted products. [Method] Ten Simmental cattle with weights of around 550 kg were selected. After slaughter and segmentation, different parts of beef （tenderloin, striploin, foreleg, hind leg, ribeye, topside）. After acid discharge for 72 h at 4 ℃, the contents of protein, fat and moisture, pH value and color index of the raw beef in different parts were determined by conventional methods, and the marbling grade was evaluated. After roast at 220 °C for 10 min, the shear force and processing loss rate of the roasted beef in different parts were measured, and the sensory quality indicators were evaluated.[Result] The fat contents, marbling grade scores, L^* values, a^* values, shear force, color scores, tenderness scores, juiciness scores, aroma scores and overall acceptability scores were significantly （P<0.05 or P<0.01） different among different parts of the raw beef, while no significant （P>0.05） differences in contents of protein and moisture, pH values, b^* values, processing loss rates and taste scores were observed. Ribeye had the highest content of fat （P<0.01）; the foreleg and hind leg had the same content of fat, and both of them were the lowest （P<0.01）; the marbling grade scores of ribeye and striploin were significantly （P<0.01） higher than those of the other parts; the L^* values of ribeye and striploin were significantly （P<0.05） higher than those of the other parts, and those of tenderloin and hind leg were significantly （P<0.05） lower than those of the other parts; the a^* values of striploin and ribeye were significantly （P<0.05） lower than those of the other parts; the shear force of ribeye and striploin were significantly （P<0.01） lower than that of the other parts; ribeye had significantly （P<0.05） higher overall acceptability scores compared with the other parts; foreleg, hind leg and topside had the same overall acceptability scores, and all of them were the lowest （P<0.05）. [Conclusion] Striploin and ribeye have higher intramuscular fat content compared with the other parts, and their roasted products perform better in tenderness, taste and overall acceptability. Accordingly, striploin and ribeye are the most suitable parts for processing roasted products in Simmental beef.

[Objective] To understand the infection status of bovine leukemia virus （BLV） at a dairy farm in Daqing City. [Method] Blood samples were collected from 107 randomly selected lactating dairy cows. The serological epidemiology investigation of BLV was carried out by ELISA, and was verified by fluorescent quantitative PCR. [Result] The BLV positive rate of ELISA was 31.78% （34/107）, and that of fluorescent quantitative PCR was 37.38% （40/107）. The coincidence rate of positive results of the two test methods was 85.00%. The dairy cows with high BLV load accounted for 26.17% of the total, and those with low BLV load accounted for 11.21% of the total. [Conclusion] The BLV infection is prevalent at a certain level at the dairy farm, and there is a higher risk of BLV spread among the dairy cows in this herd.

In order to diagnose a cat with claudication caused by high falling, basic clinical examination, laboratory examination and DR imaging examination were performed. The confirmative diagnosis was made as comminuted femoral condyle fracture of left hind limb. According to the examination, the surgical treatment plan was determined, and the cross-Kirschner needle combined with tension screw was used for surgical internal fixation. Return visit showed that the fractured end of the cat healed well, and the motor function gradually restored. It is indicated that the above fracture fixation method is effective.

[Objective] To characterize the distribution and antibiotic resistance pattern of Campylobacter coli from chickens in Henan Province. [Method] A total of 460 cloaca swab samples were collected from chicken farms to isolate the Campylobacter coli strains. The confirmative identification of the isolates was performed using PCR assay and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. The antimicrobial susceptibility test was carried out with broth micro-dilution method. [Result] A total of 42 Campylobacter coli strains were obtained with an isolating rate of 9.13%. High levels of resistance to ciprofloxacin, nalidixic acid and azithromycin were observed, and the resistant rate was 95.24%, 90.48% and 71.43%, respectively. [Conclusion] The antimicrobial resistant Campylobacter coli strains isolated from chickens are prevalent in Henan Province. Further attention should be given to the prudential use of veterinary antibiotics.

[Objective] To analyze the drug use for canine diseases in an animal hospital from year 2017 to 2018, to master the situation of canine disease prevalence and drug use in the surrounding areas, and to provide basic data for clinical medication. [Method] A retrospective study was carried out to assess the drug varieties, drug consumption sum, drug use frequency, average daily cost and drug utilization index of 4 562 outpatient prescriptions from year 2017 to 2018. [Result] Many varieties of antibiotics were used; the higher consumption sum of Fuduole bath and povidone iodine cream was found; the dexamethasone sodium phosphate injection, compound ammonium glycyrrhizinate injection and tolfenamic acid tablets were more frequently used; the sale sum of amitraz was in good synchronization with the drug use frequency; the drug utilization index from year 2017 to 2018 was less than 1. [Conclusion] Drug use for canine diseases in the animal hospital is basically reasonable.

As an important strategic plan for the development of national vocational education, the action scheme of Improving Quality and Cultivating Excellence has put forward new requirements for the cultivation of professional talents, and injected new impetus into the construction of specialty group for achieving featured and high-quality development. Based on the analysis of the necessity of modern agricultural and animal husbandry specialty group construction and grouping thought, this paper puts forward the construction paths and measures in the aspects of talent training mode innovation, curriculum system construction, curriculum teaching resources, teaching material and method reform, teachers′ innovation team construction, practice teaching base, technology and skill perform, social service ability construction, etc., in hoping to provide references for talent training of agricultural and animal husbandry specialty group.