马睾丸石蜡切片制作前期处理条件的优化

doi:10.12160/j.issn.1672-5190.2020.03.022

Animal Husbandry and Feed Science ›› 2020, Vol. 41 ›› Issue (3): 111-115.doi: 10.12160/j.issn.1672-5190.2020.03.022

Optimization of Pre-treatment Conditions for Preparation of Paraffin Sections of Horse Testis

Nariga¹,WANG Xi-sheng¹,SHEN Ying-chao¹,Caiwendaolima¹,REN Hong^1,2,Yimina¹,Taoli¹,Anaer¹,Wenderima¹,LIU Gui-qin¹,Taonala³,Gerileqimuge¹,Manglai¹

1.College of Animal Science,Inner Mongolia Agricultural University, Hohhot 010018,China;
2.Vocational and Technical College of Inner Mongolia Agricultural University,Tumed Right Banner 014109,China;
3.Agricultural,Animal Husbandry,Science and Technology Bureau of Xianghuang Banner of Xilin Gol League,Xianghuang Banner 013250,China

Received:2020-04-25 Online:2020-05-30 Published:2020-06-17

Abstract

Abstract: ［Objective］ To optimize the pre-treatment conditions of fixation and dehydration of paraffin sections preparation. ［Method］ The fixation effects of Bouin fixative solution and 4% paraformaldehyde solution at 4 ℃ and room temperature with different fixation duration were compared, and the dehydration effects of 6 h and 18 h were also contrasted. ［Result］ Either fixation with 4% paraformaldehyde solution at 4 ℃ for 24 h and then dehydration for 18 h, or fixation with Bouin fixative solution at 4 ℃ or room temperature for 24 h and then dehydration for 6 h, satisfied consequent of subsequent paraffin section preparation was obtained. ［Conclusion］ Fixation with Bouin fixative solution at room temperature for 24 h and then dehydration for 6 h can be used as the best pre-treatment plan for preparing paraffin sections of horse testis in the field.

Key words: horse testis；paraffin sections；HE staining

CLC Number:

S821.1

Nariga,WANG Xi-sheng,SHEN Ying-chao,Caiwendaolima,REN Hong,Yimina,Taoli,Anaer,Wenderima,LIU Gui-qin,Taonala,Gerileqimuge,Manglai. Optimization of Pre-treatment Conditions for Preparation of Paraffin Sections of Horse Testis[J]. Animal Husbandry and Feed Science, 2020, 41(3): 111-115.

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Optimization of Pre-treatment Conditions for Preparation of Paraffin Sections of Horse Testis

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