Abstract: Cellular culture of canine parvovirus（CPV） were subcultured in F81 cell line for proliferation and then inactivated by formaldehyde to prepare propolis-adjuvant vaccine.Goats were immunized with the vaccine,and the immune effect was observed.The results showed that the CPV antibodies were at a low level in the goats inoculated with the vaccine at a constant dosage of 5 mL per goat four times,and the HI titer on Day 28 was 1：128.The CPV antibodies were at a low level in the goats inoculated with the vaccine four times（5,10,15 and 20 mL per goat）,and the HI titer on Day 28 was 1：512,2 titers higher than in the former goats.The results showed that the inactivated propolis-adjuvant canine parvovirus cellular vaccine can induce high level of specific antibodies.