[Objective] To observe changes of plasma arginine vasopressin（AVP） levels and its relationship with altitude hypoxic acclimatization in rats under high altitude hypoxia.[Method] Fifty male SD rats were randomly divided into five groups（A,B,C,D and E）,and control group（Xi＇an region,altitude of 5 m） was also set up.The experimental groups were transported to test area by car.The rats in the group A were taken to Golmud region of Qinghai Province（altitude of 2 700 m） within 24 h.The rats in the group B were taken to Tanggula region of Tibetan area（altitude of 5 000 m） within 48 h.The rats in groups C,D and E were taken to Naqu region of Tibetan area（altitude of 4 500 m） within 72 h,1 week and 1 month,respectively.All the rats were sacrificed at different time points,and plasma AVP levels were determined by radioimmunoassay.The results were compared with those of the control group.[Result] The plasma AVP level increased after they entered the plateau.It then decreased and reached a valley after 48 h.Subsequently,it gradually picked up after 1 week and reached a peak after 1 month.[Conclusion] Under high altitude hypoxia,plasma AVP level changes with prolongation of hypoxia,maybe due to degree of hypoxia and altitude hypoxic acclimatization.

[Objective] To explore effects of bisphenol A（BPA） exposure in perinatal period on sociality and sexual behavior in adulthood of C57BL/6J mice.[Method] The female dams were orally gavaged with BPA at a dosage of 200 μg/（kg·bw） every day in the perinatal period.The behaviors of male offspring at 70 days old were observed in male-female social interaction tests.[Result] The affiliation behaviors of BPA-treated male mice to opposite sex stimulus were not changed greatly.However,compared to control mice,the BPA-treated male mice had significantly fewer mounting behaviors.[Conclusion] Perinatal exposure to BPA reduces sexual behavior of male mice.

The aim of this study was to observe changes of caecal histological structure of rex rabbits and to summarize its law of development.Caecum was collected from six rex rabbits which were reared with the same feeding and management at 1,15,30 and 60 days old,respectively.Then the caecal structure was observed under a microscope after its paraffin section was stained by HE staining.The results indicated that the height of simple columnar epithelial cells and the size of goblet cells in cecum of rex rabbits increased with aging.The distance between two semi-duplicatures gradually decreased with aging.The length and volume of duplicatures also increased slightly with aging.In addition,the number of lymphoid nodules in caecal procussus vermiformis also increased greatly.The number of lymphoid nodules and disperse lymphoid tissue also became more and more with aging,and duplicature became intensive.

The waste water from the processing of enteric coating and disebrin was treated harmless by high performance membrane separation technics to screen the project of producing feed protein powder,and the trial-produce production line was established.The result showed that the recovery rate of protein in waste water obtained over 80% under the optimization technology,and the removal rate of COD was over 70%.

A total of 90 Hunan yellow hens at 140 days old were selected.The body weight and body size（body length,chest width and tibia length） were determined.Growth curve fitting was performed.The results showed that the Logistic model and the Gompertz model well fit the growth curve of Hunan yellow hens.The fitting degree and accordance between measured values and estimated values of body weight better fit the Gompertz model,but those of body size better fit the Logistic model.

This experiment was designed to study the effect of crude extracts from Herba E.humifusae on antioxidant index of mice with spleen-deficiency.The modle was set up to produce splenasthenic syndrome by adopts lapactic and exhaustion for 60 days,the established modle was ensured by determining the activity of GSH-PX and amylase in plasma,when they significantly decline（P〈0.05）or（P〈0.01）compared with that of the former,then mice which is spleen-deficiency were randomly divided into the group of spleen-deficiency（SD） and the group of E.humifusae（EH）,and the control group（C） also was settled.The animals of EH was given decoction of 30 g/（kg·bw）,while the same dosage of sodium chloride in SD and C.60 days later,the trial animals were treated,the content of SOD and MDA was detected.The result showed that SOD activity in SD and EH is markedly lower than that of C（P〈0.05）,with the elongation of using crude extracts from Herba E.humifusae,SOD activity was markedly higher than that of SD（P〈0.05）;Meanwhile,MDA activity in SD and EH is markedly higher than that of C（P〈0.05）,with the elongation of using crude extracts from Herba E.humifusae,MDA activity was markedly higher than that of EH（P〈0.05）.The conclusion could be made that the crude extracts of Herba E.humifusae can enhanced the anti-oxidation activity obviously.

[Objective] To screen the optimal feed formula for meat-purpose rabbits（breeding rabbits and growing rabbits） and thus to reduce costs and increase economic benefits.[Method] Through contrast test,effects of different feed formulas on growth rate and disease resistance of rabbit were observed.[Result] The feed formulas were designed according to diets matching the digestibility of meat-purpose rabbit.The optimal feed formula was selected by determining nutritional indicators and growth rate.[Conclusion] To simplify feed formula for meat-purpose rabbits and to meet nutrient requirement by adjusting feed ration should be performed to promote rapid growth of meat-purpose rabbits and reduce labor costs.

In the paper,the structure and quality characteristics of crop straw was described,the impaction of the means of physics,chemistry and biology which were used to treat crop straw,their advantages and disadvantages all were introduced.According to the disadvantages of low-density of straw feed,some common stalk molding technologies and the method of granulation were also introduced.The aim was to provide the reference for reasonable utilization of crop straw,protection of enviroment,conservation of resource.

In the paper,the existed problems in Guangxi in livestock husbandry leisure feeding of sewage treatment was discussed for the recent years.Based on the actual situation of Guangxi area and combining the practice,the pattern of ＂the separation of wet and dry--methane ferments--the artificial ley--stable pond-emissions＂was put forward,and the satisfying effect was achieved.

Rice-duck farming originated in China and matured in Japan.It has such significant technological advantages as protecting eco-environment and causing great economic benefits.And it is a new model of eco-agriculture which has been popularized in rice main production regions of China.The article explores some problems about rice-duck farming technology including lacking in-depth research,no matched techniques,misunderstanding of farmers,incompatible generalization mechanism,and slow generalization progress.The paper also proposes that technical research should be strengthened,and the cultivation model should be innovated.With leading enterprises as leader of agricultural industrialization,the mechanism of ＂company ＋ base ＋ farmers＂ should be implemented,and the generalization progress should be accelerated.

Social development and requirement need construction and application of China national veterinary examination system.Before the national veterinary practice examination system was implemented,local pet-veterinarian examination has been performed in some major cities like Beijing and Shanghai.The national pet-veterinarian examination system is divided into primary,middle and high-grade levels,and the examination was composed of written examination and clinical skill.The establishment of China national pet-veterinarian examination system and examination content was described.

With the feature of novel structure and unique activity,secondary metabolites of microorganisms in the marine environment have become the important source for new drug screening,and much attention has been paid to the relevant studies.In the paper,the domestic research model on microbialmetabolites from marine-derived microorganisms and application of supercritical CO2 extraction from natural products was reviewed for the recent years.

[Objective] To optimize extraction process of efficient components in Huang-jin powder.[Method] Orthogonal test was used for optimization.Baicalin content was used as an indicator and determined by UV spectrophotometry.[Result] As indicated by the determination data,the optimum conditions for baicalin extraction are as follows：water volume,12 times;decoction,twice;once 3 h.[Conclusion] Decoction with water has simple operation,rapid determination and accurate determination results,and it thus can be used for extraction of efficient components in Huang-jin powder.

As an artificial synthetization of analgesic,dolantin is extensively used in clincal practice at home and abroad,it not only is used alonely,but also uesd by combining with others,route of administration mainly is subcutaneous,intramuscular,abdominal drugs and sublingual administration.Its function is analgesia,sedative,resisting-shivering,but the side effect also exists such as dizzy,sweating,dry mouth,nausea,tachycardia and addiction.In the paper,its chemical constitution,pharmacologic action,clinical application,side effect were reviewed to provide the reference for clinical application.

To investigate effects of drought stress on chlorophyll content and morphological indexes of potted triticale,chlorophyll content,over-ground biomass,under-ground biomass,height,spike length,spike weight and ratio of stem to leaf were determined under drought stress and irrigation treatment,respectively.The drought stress significantly decreased the over-ground biomass,under-ground biomass,height,spike length and spike weight of the potted triticale,but it did not change the ratio of stem to leaf significantly.Under drought stress,the contents of chlorophyll A,chlorophyll B and chlorophyll decreased remarkably in nutrition moment,but these three indexes showed a declining trend but did not decrease significantly in procreat moment.

Grassland rotational grazing is one of important measures to reform the traditional grazing system,scientifically and rationally use natural grassland,enhance pasture carrying capacity,and prevent the desertification of grassland.The paper introduces the concept,connotation,technical measures of rotational grazing.And benefit generated by rotational grazing was also analyzed.Xi Ujimqin Banner of Inner Mongolia should focus on planning,design and operating procedures,implement measures according to local conditions,establish different models and promote publicity in popularization of rotational grazing techniques,in order to make majority of pastoralists recognize and accept such technology.More and more pastoralists actively apply for rotational grazing project rather than passively receive rotational grazing projects.Very good results have been achieved.

Pharmacy is an important professional courses,its practice skills reflect the students actual working capacity.In the article,a new and effective assessment model,namely quantitative evaluation mode,was discussed to improve the experimental teaching effectiveness.

Food material is closely associated with natural resources,environment and human health.However,in the construction of the course ＂Food materials＂,attention should also be paid to the utilization of internet resources and full use of advanced multi-media teaching means,besides strengthening faculty construction.Meanwhile,it needs increase more site on production and circulation of raw materials for students to visit,and students＇ extra-curricular learning also should be advocate to enhance the teaching quality of the course.

Veterinary medicine specialty is highly practical and professional,and practical skills training plays the important role in personnel training of veterinary medicine specialty.In the paper,application and establishment of practice teaching system in veterinary medicine specialty from four aspect were introduced,and some feasible suggestions also put forward to provide the reference for personnel training in veterinary medicine specialty of our country.

The platform of multimedia-based teaching,platform of experiment-based teaching and multimedia website of Livestock Pathology enhanced autonomous learning ability of students,promoted their learning interest,and improved teaching quality and time effectiveness.According to the characteristics of Livestock Pathology,multimedia-based methods should be used to stimulate students＇ learning interest during experiment-based teaching process in order to improve teaching quality.

From the viewpoint of cultivating students＇ comprehensive ability and innovative ability,the importance of teaching reform of animal physiology experiment was discussed.And curriculum,teaching content,teaching methods and assessment methods and other aspects of animal physiology experiment were introduced.The research on teaching reform was also described.This paper may provide reference for teaching animal physiology.

The aim of teaching reform is to improve the teaching quality,in the paper,the integration improvement in teaching contents through emphasizing knowledge,skills,diathesis and the four unification in teaching methods to ensure the quality of application type of higher technical talented man.

In the paper,the existed problems on public elective course in universities were analyzed,and the suggestion was made to improve its quality by optimizing the course construction and resource,improving teacher＇s diathesis and enforcing the administration of examination.

[Objective] To produce fish powder with baby fish of Tilapia and develop Tilapia baby fish.[Method] The major nutrient ingredients and pepsin digestibility of fish powder were determined.[Result] The major nutrient ingredients and pepsin digestibility of the fish powder produced with Tilapia baby fish weighing less than 100 g met the requirement of special grade fish powder.[Conclusion] It is possible to produce fish powder with baby fish of Tilapia.Although its protein content and pepsin digestibility are less than those of fish powder produced with Tilapia,but it can be used as formulated feed to homogenize nutrients.

Using the uniform experiment,fresh-keeping effect of CO2,O2 and N2 at different ratios on fresh meat products at（3±1） °C was investigated.Bacterial count was determined after 18 d,and the reciprocal of logarithm of bacterial count was analyzed.The result showed the optimal ratio of CO2,O2 and N2 was 75：10：15.This modified atmosphere package made fresh port maintain second-grade freshness within 18 d.

As an important and obbligato product,its is extensively applied to the production of pastry,biscuit,snack,candy,ice cream and so on.In this paper,the product technics and situation of margarine were summarized,and developmental prospect was also forecasted with the tendency on using of non-hydrogenated butter mainly in the future.

The quality and safety of animal products threat heath and life of consumers.As a result,how to retrospect quality of animal products during the whole process from breeding to dining table has become a major issue which is faced by current animal husbandry and veterinary workers.This paper proposes some countermeasures aiming at livestock breeding,quality control in production process and construction of retrospecting system.

In order to adapt to the international market competition,the detection standards of green food of China should accord with the international standards.To develop green food industry,detection and monitoring should be strengthened,and the detection and monitoring ability should be enhanced.And many measures should been taken to solve several key technologies in production process such as fertilization and prevention of pest.Only taking these measures can promote sustainable and rapid development of green food industry.

Fishery products are an important food allergy in China.The paper introduces allergic mechanism of fishery products,and the categories and characteristics of allergen in fishery products.And the diagnostic methods for allergy in fishery products were also reviewed.

On the basis of new production mode,the paper analyzes current status of quality control system of raw milk and also proposes new ideas for improving standardized production and management system of raw milk.Quality tracking system of raw milk should be established to ensure quality and safety of raw milk.

Skeletal muscle stem cell has the characteristics of the general stem cells.It is a kind of cells which can be updated and reproduced by themselves,and it can differentiate into various cells with specific functions.Thus it can be used as seed cells in tissue engineering and has important and available value.This article reports the recent development in induced differentiation of skeletal muscle stem cells in order to offer a theoretical basis for further study on skeletal muscle stem cells.

The paper introduces research progress in transplantation technology of frozen sex-controlled embryos from Holstein cows to cattle.And it also describes extensive amplification prospects of the large scale transplantation in dairy industry in China.

The parturition of 60 boars（Landrace,Yorkshire and Duroc） and more than 300 sows（Landrace,Yorkshire and Duroc） in the largest original pig breeding farm of Anhui Province were collected for analysis.Artificial insemination and natural mating were compared between these species to study the effects of different mating manners on litter traits in sows of different species.And correlation between parity and litter size was analyzed.Compared to the natural mating,the artificial insemination affected litter size or viable litter size significant（P〈0.05）.The litter size or viable litter size was significant different between artificially inseminated sows and naturally mating sows（P〈0.05）.The results indicated that artificial insemination can effectively substitute natural mating in breeding pigs although the former require highly strict operation.

Sperm-mediated gene transfer（SMGT） which was developed in 1989 has attracted great attention from many scientists due to its high efficiency,low cost and simple operation.The mechanism,frequently used method and influencing factor of SMGT were presented.

The FUT1 Genotypes of 118 sows with hybridization between yorkshire and landrace were detected by using PCR-RFLP,and the correlation to reproductive traits were analysized.The frequency of AA,AG,GG genotype in FUT1was 0.11,0.37,0.52,respectively,there is no significant difference in litter size and the number of live birth among the three genetype（P 〉0.05）.

By comparing the three extraction methods of genomic DNA,the best extraction method of lucilia genomic DNA was screened.As shown by determination of DNA concentration and electrophoresis,the Collines method was the best extraction method and could be used for extraction of lucilia genomic DNA.

Tyrosinase related protein 1（TYRP1） is involved in regulation and control of melanin synthesis,and it is a member of tyrosinase family.The paper reviews melanin synthesis and its research progress.The structure,function and application in species of TYRP1 gene which affects melanin synthesis were also reviewed.

Clostridium perfringens has long been recognized as an important pathogen of zoonosis and can cause sitotoxis in humans and gastrointestinal diseases in domestic animals.Some progresses in minor toxin,enterotoxin and enzyme of Clostridium perfringens have been achieved recently.This paper introduces progresses in composition,structure,chemical and physical properties,molecular biology of minor toxin,enterotoxin and enzyme of Clostridium perfringens.

Cow breeding industrialization management is a new manner of production management,which is developed in the process of market economy development.The advantages and restriction factors of cow breeding in Xilinhot region of Inner Mongolia should be analyzed comprehensively and deeply.The experience in development of international dairy industry should also be learned so as to achieve sustainable and steady development of dairy industry of Xilinhot region.And this is significant to realize the object of increasing herdsman＇s income,and it also has theoretical and practical significance for regional dairy industry in global market integration environment.Taking the actual situation of cow breeding industrial production management in Xilinhot as background,the restriction factors,regional comparative advantages and behaviors of general stakeholders were analyzed according to the current status of cow culture（Low level and no fast increasing social efficiency）.And the ways to promote cow breeding industrial production management were also proposed.

A total of 7 500 broilers were randomly divided into two groups.The broilers in one group were fed with common breeding technique,and those in another group were fed in fermentation bed.According to the established program strictly,food consumption,number of deaths and body weight were recorded every day.According to the statistical data of 45 d,the fermentation bed technique had much better advantages than the common breeding technique in decreasing feed conservation ratio and mortality,improving feeding environment,and avoiding drug use.

[Objective] The effect of Combination of Chinese Herbs and western medicine on Eperythrozoonosis was explored in the trial.[Methods] Swines infected with Eperythrozoon suis were divided into control group（C）,Chinese herb group（CH）,western medicine group（WM） and Combination of Chinese Herbs and western medicine group（CCHWM）.C were as symptomatic treatment without using any medicine;CH with Chinese herbal medicine compound preparation at the dosage of 75 mL/（kg·bw·d）;WM with Bernier at the dosage of 5～7 mg/（kg·bw·d）by intramuscular injection;CCHWM with Bernier and Chinese herbal medicine compound preparation at the dosage of 75 mL/（kg·bw·d）,after treated continuously for 5 days,the indexes of clinical symptoms,blood convention,erythrocyte osmotic fragility,biochemical indices,infection rate,negative rate,cure rate and efficiency rate among them were observed and contrasted.[Results] Through the treatment,all indexes in CCHWM obtained the normal.[Conclusion] The effect was achieved by the treatment of CCHWM.

Cellular culture of canine parvovirus（CPV） were subcultured in F81 cell line for proliferation and then inactivated by formaldehyde to prepare propolis-adjuvant vaccine.Goats were immunized with the vaccine,and the immune effect was observed.The results showed that the CPV antibodies were at a low level in the goats inoculated with the vaccine at a constant dosage of 5 mL per goat four times,and the HI titer on Day 28 was 1：128.The CPV antibodies were at a low level in the goats inoculated with the vaccine four times（5,10,15 and 20 mL per goat）,and the HI titer on Day 28 was 1：512,2 titers higher than in the former goats.The results showed that the inactivated propolis-adjuvant canine parvovirus cellular vaccine can induce high level of specific antibodies.

The suspected cases of gosling plague were dissected to determine the disease by pathological changes in the local goose field initially,then a wild strain goose parvovirus was isolated successfully from the organs of dead gosling using virus reproduction method.The pathogenicity test,neutralization test of goose embryos,infection test of gosling and agar diffusion reaction were carried out to identify it.The results showed that the viral strain was identified as goose parvovirus with high pathogenicity.