Abstract: There are a large number of splicing genes in higher eukaryotic genome,but these mRNA is of non-splicing structure.RNA splicing means removing introns in the transformation process.RNA splicing,especially variable RNA splicing,is an important part of regulation of eukaryotic gene expression.The paper describes the characteristics,function and regulation of RNA splicing,alternative splicing genes,and identification of splicing forms.Some samples were taken to introduce the application of alternative RNA splicing.