Abstract: Coenzyme Q10 is an important biologically active substance and widely present in organisms. It can enhance human immunity and antioxidant capacity and can also delay aging. At present, biosynthesis is a main preparation method and also future trend to produce coenzyme Q10 in industrial production. The eoenzyme Q10 has been applied in food, health products, cosmetics and other fields in addition to medicine field. The paper introduces progress in preparation and application of coenzyme Q10.