北方农业学报 ›› 2020, Vol. 48 ›› Issue (5): 95-103.doi: 10.12190/j.issn.2096-1197.2020.05.17

• 园艺·草业 • 上一篇    下一篇

低温胁迫对西瓜种子萌发的影响

沈虹, 孟佳丽, 吴绍军, 余翔   

  1. 江苏省农业科学院 宿迁农科所,江苏 宿迁 223800
  • 收稿日期:2020-08-26 出版日期:2020-10-20 发布日期:2020-12-17
  • 通讯作者: 余 翔(1983—),男,副研究员,硕士,主要从事蔬菜育种及栽培技术的研究工作。
  • 作者简介:沈 虹(1986—),女,助理研究员,硕士,主要从事蔬菜育种及栽培技术的研究工作。
  • 基金资助:
    江苏省农业重大新品种创制项目(PZCA201716); 宿迁市农业科技自主创新资金项目(SQCX201908)

Effects of low temperature stress on watermelon seed germination

SHEN Hong, MENG Jiali, WU Shaojun, YU Xiang   

  1. Suqian Institute of Agricultural Sciences,Jiangsu Academy of Agricultural Sciences,Suqian 223800,China
  • Received:2020-08-26 Online:2020-10-20 Published:2020-12-17

摘要: 【目的】研究低温对不同西瓜材料萌发期各指标的影响,筛选西瓜耐低温种质材料,为西瓜耐低温品种选育提供理论参考。【方法】以39个西瓜品种(系)、组合为试验材料,利用培养箱模拟不同低温胁迫(15/10 ℃、15 ℃和17 ℃),分析低温胁迫对西瓜种子发芽势、发芽率、胚根长、活力指数、冷害指数和恢复能力的影响,采用极点排序法对不同西瓜材料的耐低温性进行综合评价。【结果】低温胁迫下,西瓜种子的发芽势、相对发芽率、相对胚根长和相对活力指数均降低,且胁迫温度越低,抑制越明显。西瓜种子发芽势、相对发芽率、相对胚根长和相对活力指数均与耐低温综合评价得分极显著相关,且相对活力指数与综合评价得分相关性最高(r=0.949)。不同西瓜材料萌芽期耐低温能力不同,17 ℃下,发芽势、相对发芽率、相对胚根长、相对活力指数 4 项指标在不同西瓜材料间差异显著。选用上述4个指标和冷害指数指标,采用极点排序法得出各西瓜材料的综合评价得分,170-1综合得分大于5,为耐低温材料;迁丽4号、苏蜜1667、迁丽1号、迁丽4号反交、迁美1号、135-2、161-5、17A-81×17A-82综合得分小于2,为低温敏感材料;其余材料为中度耐低温材料。【结论】低温抑制了西瓜种子的萌发,17 ℃可作为鉴定不同西瓜材料萌芽期耐低温性的适宜温度,不同低温耐受性的西瓜材料可为西瓜耐低温品种选育和基础理论研究提供试验材料。

关键词: 西瓜, 低温, 种子萌发, 冷害

Abstract: 【Objective】 The effects of low temperature on the germination indexes of different watermelon materials were studied,and the low temperature tolerant germplasm materials were screened to provide theoretical reference for the breeding of low temperature tolerant watermelon varieties.【Methods】Thirty-nine watermelon varieties(lines)and combinations were used as experimental materials,and a few incubators were used to simulate different low temperature stresses(15/10 ℃,15 ℃ and 17 ℃).The effects of low temperature stress on germination potential,germination rate,radicle length,vigor index,chilling injury index and recovery ability of watermelon seeds were analyzed,furthermore,and the low temperature tolerance of different watermelon materials was evaluated by pole-sorting method.【Results】Germination potential,relative germination rate,relative radicle length and relative vigor index were decreased under low temperature stress,and the inhibition was more obvious with the decrease of stress temperature.The germination potential,relative germination rate,relative radicle length and relative vigor index of watermelon seeds were significantly correlated with the comprehensive evaluation score of low temperature tolerance,and the relative vigor index and the evaluation scores exhibited the highest correlation(r=0.949).Different watermelon materials had different tolerance to low temperature during germination.At 17 ℃,there were significant differences in germination potential,relative germination rate,relative radicle length and relative vigor index among different watermelon materials.The four indexes and chilling injury index were selected and the comprehensive evaluation score of each watermelon material was obtained by using the pole sorting method.The results showed that the comprehensive evaluation score of 170-1 was higher than 5,which was a low temperature resistant material.The comprehensive score of Qianli 4,Sumi 1667,Qianli 1,reciprocals of Qianli 4,Qianmei 1,135-2,161-5,17A-81×17A-82 were lower than 2,which were low temperature sensitive materials,anothers were medium low temperature resistant materials.【Conclusion】Low temperature inhibited the germination of watermelon seeds,17 ℃ could be used as the suitable temperature for identification of low temperature tolerance of different watermelon materials at germination stage,watermelon materials with different low temperature tolerance could provide experimental materials for breeding of low temperature tolerant varieties and basic theoretical research.

Key words: Watermelon, Low temperature, Germination, Chilling injury

中图分类号: 

  • S651