北方农业学报 ›› 2022, Vol. 50 ›› Issue (4): 55-59.doi: 10.12190/j.issn.2096-1197.2022.04.08

• 种质资源 · 植物保护 • 上一篇    下一篇

陕西省番茄黄化曲叶病毒寄主范围测定

刘晨, 潘嵩, 魏佩瑶, 陈志杰, 李英梅   

  1. 陕西省生物农业研究所,陕西 西安 710043
  • 收稿日期:2022-06-15 出版日期:2022-08-20 发布日期:2022-09-30
  • 通讯作者: 李英梅
  • 作者简介:李英梅(1975—),女,研究员,硕士,主要从事病虫害绿色防控的研究工作。
    刘 晨(1988—),女,助理研究员,硕士,主要从事植物保护方面的研究工作。
  • 基金资助:
    陕西省重点研发计划一般项目(2022NY-126);西安市科技局农业技术研发项目(20NYYF0042);陕西省科学院品牌计划项目(2022K-02)

Host range determination of tomato yellow leaf curl virus in Shaanxi Province

LIU Chen, PAN Song, WEI Peiyao, CHEN Zhijie, LI Yingmei   

  1. Bio-agriculture Institute of Shaanxi,Xi′an 710043,China
  • Received:2022-06-15 Online:2022-08-20 Published:2022-09-30
  • Contact: LI Yingmei

摘要:

【目的】明确番茄黄化曲叶病毒(tomato yellow leaf curl virus, TYLCV)的寄主范围,为防控陕西省番茄黄化曲叶病毒提供理论基础。【方法】对陕西省大荔县、泾阳县、杨凌示范区3个基地不同时间段番茄产区周边11种作物的158份样品、32种常见杂草的283份样品进行TYLCV的PCR检测。【结果】黄瓜、辣椒、茄子、豇豆及杂草苋菜均携带TYLCV,为番茄黄化曲叶病毒的中间寄主,是该病毒的重要侵染来源。序列比对结果进一步确认这些作物上的病原为番茄黄化曲叶病毒。【结论】TYLCV可以侵染黄瓜、辣椒、茄子、豇豆这4种作物及杂草苋菜,为TYLCV的中间寄主。

关键词: 陕西省, 番茄黄化曲叶病毒, 寄主范围

Abstract:

【Objective】To clarify the host range of tomato yellow leaf curl virus(TYLCV)and to offer a theoretical foundation for the prevention and control of TYLCV in Shaanxi Province.【Methods】158 samples of 11 crops and 283 samples of 32 common weeds from Dali County,Jingyang County,and Yangling Demonstration Area in Shaanxi Province were collected at different time points and tested for TYLCV by PCR.【Results】Cucumber,pepper,eggplant,cowpea and weed amaranth all carried TYLCV,were the intermediate host of TYLCV and important sources of infection. Sequence alignment results further confirmed that the pathogen on these crops was tomato yellow leaf curl virus.【Conclusion】TYLCV could infect cucumber,pepper,eggplant,cowpea and amaranth. These were the intermediate hosts of TYLCV.

Key words: Shaanxi Province, Tomato yellow leaf curl virus, Host range

中图分类号: 

  • S436.412