水杨酸诱导水稻抗稻瘟病的机制研究

doi:10.12190/j.issn.2096-1197.2022.04.07

摘要/Abstract

摘要：

【目的】阐明水杨酸诱导水稻抗稻瘟病的分子机制。【方法】以Hwayoung水稻幼苗为试验材料,测定水杨酸对稻瘟病菌的体外抑制作用。用水杨酸(无菌水为对照)诱导水稻幼苗后接种稻瘟病菌JC2,研究水稻全基因组组蛋白乙酰化的变化、水稻病程相关基因表达的变化。【结果】水杨酸在体外对稻瘟病菌的生长速率、孢子量及孢子萌发率均无明显抑制作用。水稻幼苗在不同浓度的水杨酸诱导后,组蛋白H3K9ac、H4K5ac的修饰水平呈现上升趋势,水稻对稻瘟病菌的抗性也有所增强。与对照植株相比,0.05 mmol/L 水杨酸接菌2 d诱导处理植株(接菌后24 h取样)中,OsPR1b、OsPAL、OsNH1、WRKY13的表达量都出现了极显著上升(P<0.01)。【结论】水稻在受到水杨酸诱导后,组蛋白乙酰化修饰水平升高,抗稻瘟病能力增强。

关键词: 水稻, 水杨酸, 稻瘟病, 诱导抗性, 组蛋白乙酰化

Abstract:

【Objective】To clarify the molecular mechanism of salicylic acid induced rice blast resistance.【Methods】Using Hwayoung rice seedlings as test material,the in vitro inhibitory effect of salicylic acid on Magnaporthe oryzae was evaluated. Rice seedlings were induced by salicylic acid(sterile water as control)and then inoculated with Magnaporthe oryzae JC2. The changes in histone acetylation and gene expression related to rice disease course were studied.【Results】Salicylic acid had no significant inhibitory effect on the in vitro growth rate,spore quantity,and spore germination rate of Magnaporthe oryzae. The histone modification levels of H3K9ac and H4K5ac showed an increased trend when the rice seedlings were induced by different concentrations of salicylic acid,and the resistance to rice blast was also improved. Compared with the control plants,in 0.05 mmol/L salicylic acid induced and inoculated 2 d plants(sampled 24 h after inoculation),the expression levels of OsPR1b、OsPAL、OsNH1 and WRKY13 increased extreme significantly(P<0.01).【Conclusion】After rice plants were induced by salicylic acid,the histone acetylation modification level was elevated and the resistance to rice blast was improved.

Key words: Rice, Salicylic acid, Rice blast, Induced resistance, Histone acetylation

中图分类号:

S435.111.41

付佳, 刘婷婷, 张辉, 蒋善强, 周溧, 马丹, 田雪军, 朱德艳, 徐艳. 水杨酸诱导水稻抗稻瘟病的机制研究[J]. 北方农业学报, 2022, 50(4): 47-54.

FU Jia, LIU Tingting, ZHANG Hui, JIANG Shanqiang, ZHOU Li, MA Dan, TIAN Xuejun, ZHU Deyan, XU Yan. Study on the mechanism of salicylic acid inducing rice blast resistance[J]. Journal of Northern Agriculture, 2022, 50(4): 47-54.

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