二球悬铃木COL5 同源基因及其表达模式分析

doi:10.12190/j.issn.2096-1197.2022.05.02

北方农业学报 ›› 2022, Vol. 50 ›› Issue (5): 10-18.doi: 10.12190/j.issn.2096-1197.2022.05.02

• 作物遗传育种 · 分子生物学 • 上一篇下一篇

二球悬铃木COL5 同源基因及其表达模式分析

王洋¹, 谢宇¹, 孙海莲¹, 石磊¹, 邱晓¹, 刘亚红¹, 常乐², 高凤云³

1.内蒙古自治区农牧业科学院综合试验示范中心，内蒙古呼和浩特 010031
2.华中农业大学，湖北武汉 430070
3.内蒙古自治区农牧业科学院特色作物研究所，内蒙古呼和浩特 010031

收稿日期:2022-06-10 出版日期:2022-10-20 发布日期:2023-01-10
通讯作者: 高凤云
作者简介:高凤云（1977—），女，研究员，博士，主要从事作物遗传育种研究工作。
王洋（1996—），女，研究实习员，学士，主要从事生态学研究工作。
基金资助:
内蒙古自治区自然科学基金项目(2020MS03095)

Analysis of COL5 homologous genes and the expression pattern in Platanus acerifolia

WANG Yang¹, XIE Yu¹, SUN Hailian¹, SHI Lei¹, QIU Xiao¹, LIU Yahong¹, CHANG Le², GAO Fengyun³

1. Center for Comprehensive Test and Demonstration，Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences，Hohhot 010031，China
2. Huazhong Agricultural University，Wuhan 430070，China
3. Institute of Special Crops，Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences，Hohhot 010031，China

Received:2022-06-10 Online:2022-10-20 Published:2023-01-10
Contact: GAO Fengyun

摘要/Abstract

摘要：

【目的】探究二球悬铃木COL5 同源基因在表达水平上与休眠调控的相关性。【方法】选定CO-Like基因家族中的COL5作为研究对象，以二球悬铃木成年植株为材料，设计基因特异引物，对COL5同源基因进行全长扩增；通过一年生二球悬铃木幼苗群体的休眠环境因子调控试验，分析该基因在单一短日照（24 ℃，8 h/16 h）、单一低温（8 ℃，12 h/12 h）和低温短日照（8 ℃，8 h/16 h）条件下的响应表达模式。【结果】PaCOL5-1全长为1 444 bp，包括1 134 bp CDS区，推测编码377个氨基酸；PaCOL5-2全长为1 371 bp，包括1 131 bp CDS区，推测编码376个氨基酸；PaCOL5-3全长为1 186 bp，包括1 125 bp CDS区，推测编码374个氨基酸。单一低温会抑制PaCOL5-1的表达，PaCOL5-1和PaCOL5-3均对低温短日照条件有所响应，在处理7 d后会出现1个峰值；PaCOL5-2对低温短日照条件的响应不明显。【结论】PaCOL5-1、PaCOL5-2 和PaCOL5-3基因对单一环境因子的响应表达程度不同，PaCOL5-1和PaCOL5-3基因可能与休眠环境因子的响应有关。

关键词: 二球悬铃木, COL5, 休眠调控, 表达分析

Abstract:

【Objective】To investigate the relationship between Platanus acerifolia expression level of COL5 homologous genes and dormancy regulation.【Methods】COL5 in the CO-Like gene family was selected as the research object. Gene-specific primers were used to amplify the full length of COL5 homologous genes in Platanus acerifolia adult plants. Platanus acerifolia annual seedling regulation experiment of dormancy environment was used to analyze the expression pattern of the genes under the conditions of short day light alone（24 ℃，8 h/16 h），low temperature alone（8 ℃，12 h/12 h），or low temperature and short day light（8 ℃，8 h/16 h）.【Results】The full length of PaCOL5-1 was 1 444 bp，including 1 134 bp CDS region，which was presumed to encode 377 amino acids. The full length of PaCOL5-2 was 1 371 bp，including 1 131 bp CDS region，which was presumed to encode 376 amino acids. The full length of PaCOL5-3 was 1 186 bp，including 1 125 bp CDS region，which was presumed to encode 374 amino acids. Low temperature alone inhibited the expression of PaCOL5-1. Both PaCOL5-1 and PaCOL5-3 responded to low temperature and short day light conditions，and had a peak after 7 d treatment. The response of PaCOL5-2 to low temperature and short day light conditions was not obvious.【Conclusion】PaCOL5-1，PaCOL5-2，and PaCOL5-3 had different gene expression levels in response to a single environmental stimulus. PaCOL5-1 and PaCOL5-3 genes may be involved in the response of dormant environmental factors.

Key words: Plattanus acerifolia, COL5, Dormancy regulation, Expression analysis

中图分类号:

S687.1

王洋, 谢宇, 孙海莲, 石磊, 邱晓, 刘亚红, 常乐, 高凤云. 二球悬铃木COL5 同源基因及其表达模式分析[J]. 北方农业学报, 2022, 50(5): 10-18.

WANG Yang, XIE Yu, SUN Hailian, SHI Lei, QIU Xiao, LIU Yahong, CHANG Le, GAO Fengyun. Analysis of COL5 homologous genes and the expression pattern in Platanus acerifolia[J]. Journal of Northern Agriculture, 2022, 50(5): 10-18.

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二球悬铃木COL5 同源基因及其表达模式分析

Analysis of COL5 homologous genes and the expression pattern in Platanus acerifolia

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