盐度对养殖大麻哈鱼生长性能、鳃三磷酸腺苷酶和肝脏抗氧化功能的影响

doi:10.12190/j.issn.2096-1197.2023.02.13

北方农业学报 ›› 2023, Vol. 51 ›› Issue (2): 108-115.doi: 10.12190/j.issn.2096-1197.2023.02.13

盐度对养殖大麻哈鱼生长性能、鳃三磷酸腺苷酶和肝脏抗氧化功能的影响

李培伦^1,2, 刘嘉成^1,3, 鲁万桥¹, 王继隆¹

1.中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨 150070;
2.黑龙江省冷水性鱼类种质资源及增养殖重点开放实验室,黑龙江哈尔滨 150070;
3.东北农业大学动物科学技术学院,黑龙江哈尔滨 150070

收稿日期:2023-03-01 出版日期:2023-04-20 发布日期:2023-07-05
通讯作者: 王继隆（1984—）,男,副研究员,硕士,主要从事渔业资源生物学的研究工作。
作者简介:李培伦（1989—）,男,助理研究员,硕士,主要从事冷水性鱼类增养殖的研究工作。
基金资助:
农业农村部财政专项物种资源保护项目（2017-2021）; 中央级公益性科研院所基本科研业务费专项（HSY201712Q）

Effects of salinity on growth performance,gill ATPase activities and liver antioxidant function in farmed chum salmon（Oncorhynchus keta）

LI Peilun^1,2, LIU Jiacheng^1,3, LU Wanqiao¹, WANG Jilong¹

1. Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China;
2. Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province,Harbin 150070,China;
3. College of Animal Science and Technology,Northeast Agricultural University,Harbin 150070,China

Received:2023-03-01 Online:2023-04-20 Published:2023-07-05

摘要/Abstract

摘要： 【目的】探究盐度对养殖大麻哈鱼生长性能、鳃三磷酸腺苷酶（ATP酶）和肝脏抗氧化功能的影响。【方法】设置淡水（CK）、盐度8‰、盐度16‰和盐度24‰处理,42 d后统计大麻哈鱼（2龄）的生长性能,测定鳃ATP酶活力,肝脏超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化酶（GSH-PX）、酸性磷酸酶（ACP）和碱性磷酸酶（AKP）活力,以及肝脏总抗氧化能力（T-AOC）和丙二醛（MDA）含量。【结果】盐度8‰处理大麻哈鱼的终末体质量、体质量增长率和特定生长率分别为374.95 g、29.41%和0.61 %/d,显著高于CK和盐度24‰处理（P<0.05）;盐度16‰处理的终末体质量、体质量增长率和特定生长率与其他各处理间无显著差异（P>0.05）。大麻哈鱼鳃Na⁺/K⁺-ATP酶、Ca²⁺/Mg²⁺-ATP酶活力均随盐度的升高呈现先上升后下降的趋势,其中,盐度8‰处理酶活力最高,分别为1.89、1.46 U/mg。肝脏SOD、CAT活力均随盐度的升高呈现先上升后下降的趋势,其中,盐度16‰处理酶活力最高,分别为244.49、34.16 U/mg;GSH-PX活力随盐度的升高呈现先下降后升高的趋势,CK、盐度8‰、盐度16‰处理显著高于盐度24‰处理（P<0.05）;ACP活力随盐度的升高呈现先上升后下降的趋势,各处理间无显著差异（P>0.05）;AKP活力随盐度升高逐渐下降,CK显著高于其他各处理（P<0.05）。CK的MDA含量显著高于其他各处理（P<0.05）;T-AOC在各处理间无显著差异（P>0.05）。【结论】大麻哈鱼在2龄生长阶段养殖水体中适宜的盐度应介于8‰~16‰。

关键词: 大麻哈鱼, 生长性能, 鳃, 三磷酸腺苷酶, 肝脏, 抗氧化功能

Abstract: 【Objective】To explore the effects of salinity on growth performance,gill ATPase activities,and liver antioxidant function in farmed chum salmon（Oncorhynchus keta）.【Methods】Four treatments were set up including freshwater（CK）,salinity 8‰,salinity 16‰ and salinity 24‰. The growth performance of chum salmon（2-year-old） after 42 d were calculated,and the gill ATPase activities,liver superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-PX）,acid phosphatase （ACP） and alkaline phosphatase （AKP） activities,as well as the total antioxidant capacity （T-AOC） and malondialdehyde（MDA） content in the liver were measured.【Results】The final body mass,body mass growth rate and specific growth rate of chum salmon in salinity 8‰ treatment were 374.95 g,29.41% and 0.61 %/d,respectively,significantly higher （P<0.05） than CK and salinity 24‰ treatment. Salinity 16‰ treatment had no significant difference （P>0.05） in final body mass,body mass growth rate and specific growth rate,compared with other treatments. Chum salmon gill Na⁺/K⁺-ATPase and Ca²⁺/Mg²⁺-ATPase activities increased first and then decreased with the increase of salinity. Among them,salinity 8‰ treatment had the highest enzyme activities of 1.89 U/mg and 1.46 U/mg,respectively. With the increase of salinity,the activities of SOD and CAT in liver increased first and then decreased,with salinity 16‰ treatment being the highest at 244.49 U/mg and 34.16 U/mg,respectively. The activity of GSH-PX decreased first and then increased with the increase of salinity,with CK,salinity 8‰,and salinity 16‰ treatment significantly higher（P<0.05） than salinity 24‰ treatment. With the increase of salinity,ACP activity increased first and then decreased with no significant differences（P>0.05） between the treatments. With the increase of salinity,AKP activity gradually decreased with CK significantly higher（P<0.05） than other treatments. The content of MDA in CK was significantly higher（P<0.05） than that in other treatments. And there was no significant difference（P>0.05） in T-AOC among the treatments.【Conclusion】The suitable aquaculture water salinity for 2-year-old chum salmon was between 8‰ and 16‰.

Key words: Chum salmon（Oncorhynchus keta）, Growth performance, Gill, ATPase, Liver, Antioxidant function

中图分类号:

S917.4

李培伦, 刘嘉成, 鲁万桥, 王继隆. 盐度对养殖大麻哈鱼生长性能、鳃三磷酸腺苷酶和肝脏抗氧化功能的影响[J]. 北方农业学报, 2023, 51(2): 108-115.

LI Peilun, LIU Jiacheng, LU Wanqiao, WANG Jilong. Effects of salinity on growth performance,gill ATPase activities and liver antioxidant function in farmed chum salmon（Oncorhynchus keta）[J]. Journal of Northern Agriculture, 2023, 51(2): 108-115.

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盐度对养殖大麻哈鱼生长性能、鳃三磷酸腺苷酶和肝脏抗氧化功能的影响

Effects of salinity on growth performance,gill ATPase activities and liver antioxidant function in farmed chum salmon（Oncorhynchus keta）

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