桔梗bHLH基因家族鉴定与表达分析

doi:10.12190/j.issn.2096-1197.2026.01.05

北方农业学报 ›› 2026, Vol. 54 ›› Issue (1): 43-55.doi: 10.12190/j.issn.2096-1197.2026.01.05

• 作物遗传育种·分子生物学 • 上一篇下一篇

桔梗bHLH基因家族鉴定与表达分析

陈子怡, 汪晓蕊, 何涵, 李子墨, 夏自玉, 黄文静, 孙晓春

陕西中医药大学陕西中药资源产业化省部共建协同创新中心/秦药特色资源研究开发国家重点实验室（培育）,陕西咸阳 712083

收稿日期:2025-09-19 出版日期:2026-02-20 发布日期:2026-04-01
通讯作者: 孙晓春（1985—）,女,副教授,博士,硕士生导师,主要从事中药资源方面的研究工作。
作者简介:陈子怡（2006—）,女,本科生,专业为药物制剂。
基金资助:
国家自然科学基金青年科学基金项目（81703656）; 陕西省科技厅自然科学基础研究项目（S2025-JC-YB-0636）; 陕西省教育厅项目（23JY026）

Identification and expression analysis of the bHLH gene family of Platycodon grandiflorus

CHEN Ziyi, WANG Xiaorui, HE Han, LI Zimo, XIA Ziyu, HUANG Wenjing, SUN Xiaochun

Provincial and Ministerial Co-construction Collaborative Innovation Center of Shaanxi Chinese Medicine Resources Industrialization/State Key Laboratory of Research and Development of Qin Medicine Characteristic Resources （Cultivation）,Shaanxi University of Chinese Medicine,Xianyang 712083,China

Received:2025-09-19 Online:2026-02-20 Published:2026-04-01

摘要/Abstract

摘要： 【目的】探究桔梗bHLH基因家族的特征和功能。【方法】对桔梗bHLH基因家族进行理化性质、系统进化树、保守基序、基因结构、顺式作用元件和共线性分析,采用实时荧光定量PCR（qRT-PCR）方法检测bHLH基因在桔梗组织的表达模式。【结果】桔梗bHLH基因家族共鉴定到94个成员,分为9个亚家族,分布于8条染色体上。保守基序分析显示,Motif1和Motif2是该家族的主要保守基序,基因结构具有多样性,含有1~11个外显子;启动子顺式作用元件分析发现,其包含与光响应、植物激素、胁迫相关的响应元件;共线性分析检测到18对bHLH重复基因对。组织特异性表达分析显示,PgbHLH65、PgbHLH8和PgbHLH72在茎中的相对表达量较高,PgbHLH82和PgbHLH20在根中的相对表达量较高,PgbHLH10、PgbHLH52和PgbHLH91在叶中的相对表达量较高。【结论】不同bHLH基因在桔梗根、茎、叶中可能发挥特异性功能。

关键词: 桔梗, bHLH基因, 转录因子, 生物信息学, 组织表达模式

Abstract: 【Objective】 To investigate the characteristics and functions of the bHLH gene family of Platycodon grandiflorus. 【Methods】 Analyses of physicochemical properties,phylogenetic tree,conserved motifs,gene structures,cis-regulatory elements,and collinearity analysis were conducted for the bHLH gene family of P. grandiflorus. Quantitative real-time PCR （qRT-PCR） was used to examine the expression patterns of bHLH genes in tissues of P. grandiflorus. 【Results】 A total of 94 members were identified in the bHLH gene family of P. grandiflorus,which were classified into nine subfamilies and distributed across eight chromosomes. Conserved motif analysis showed that Motif1 and Motif2 were the major conserved motifs of this family. The gene structures were diverse,containing 1-11 exons. Analysis of cis-regulatory elements in the promoters revealed that they contained response elements associated with light responsiveness,plant hormones,and stress. Collinearity analysis identified 18 pairs of duplicated bHLH genes. Tissue-specific expression analysis indicated that PgbHLH65,PgbHLH8,and PgbHLH72 were higher relative expression in stems,PgbHLH82 and PgbHLH20 showed higher relative expression in roots,and PgbHLH10,PgbHLH52,and PgbHLH91 exhibited higher relative expression in leaves. 【Conclusion】 Different bHLH genes may perform specific functions in the roots,stems,and leaves of P. grandiflorus.

Key words: Platycodon grandiflorus, bHLH gene, Transcription factor, Bioinformatics, Tissue expression pattern

中图分类号:

S567.239

陈子怡, 汪晓蕊, 何涵, 李子墨, 夏自玉, 黄文静, 孙晓春. 桔梗bHLH基因家族鉴定与表达分析[J]. 北方农业学报, 2026, 54(1): 43-55.

CHEN Ziyi, WANG Xiaorui, HE Han, LI Zimo, XIA Ziyu, HUANG Wenjing, SUN Xiaochun. Identification and expression analysis of the bHLH gene family of Platycodon grandiflorus[J]. Journal of Northern Agriculture, 2026, 54(1): 43-55.

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桔梗bHLH基因家族鉴定与表达分析

Identification and expression analysis of the bHLH gene family of Platycodon grandiflorus

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