北方农业学报 ›› 2019, Vol. 47 ›› Issue (2): 73-78.doi: 10.3969/j.issn.2096-1197.2019.02.12

• 著者文摘 • 上一篇    下一篇

阿尔山落叶松根际土壤固氮菌多样性研究

郭振华[1];陈立红[1]   

  1. [1]内蒙古农业大学农学院,内蒙古呼和浩特010019
  • 收稿日期:2019-02-25 出版日期:2019-02-19 发布日期:2019-08-19
  • 作者简介:郭振华(1993—),男,硕士研究生,研究方向为固氮菌多样性;通讯作者:陈立红(1969-),女,教授,博士,主要从事土壤微生物的研究工作。
  • 基金资助:
    国家自然科学基金项目(31260173).

Diversity of nitrogen-fixing bacteria isolated from Larix gmelinii rhizosphere soil in Arxan

GUO Zhenhua[1];CHEN Lihong[1]   

  1. [1]Agricultural College,Inner Mongolia Agricultural University,Hohhot 010019,China
  • Received:2019-02-25 Online:2019-02-19 Published:2019-08-19

摘要: 为研究阿尔山地区落叶松根际固氮菌的多样性,试验采用平板分离法,结合16S rDNA序列比对,对落叶松根际土壤固氮菌组成类群及其多样性进行了分析。试验结果表明:自落叶松根际土壤共分离纯化细菌菌株28株,分属于10属18种,分别为假单胞菌属(Pseudomonas)、沙雷菌属(Serratia)、伯克氏菌属(Burkholderia)、根瘤菌属(Rhizobium)、叶杆菌属(Phyllobacterium)、类芽孢杆菌属(Paenibacillus)、芽孢杆菌属(Bacillus)、红球菌属(Rhodococcus)、小杆菌属(Curtobacterium)、节杆菌属(Arthrobacter)。其中Pseudomonas、Phyllobacterium、Arthrobacter为优势菌属。研究结果阐明了阿尔山地区落叶松根际固氮菌的组成及其多样性。

关键词: 阿尔山;落叶松;固氮菌;16SrDNA

Abstract: In this paper plate isolation method,combined with 16S rDNA sequence analysis,were used to investigate the diversity and community structure of rhizosphere nitrogen-fixing bacteria isolated from Larix gmelinii in Arxan National Forest Park.A total of 28 isolates were obtained from L.gmelinii rhizosphere soil and their 16s rDNAs were sequenced and analyzed.The isolates were classified into 10 genera and 18 species.The genera isolated included Pseudomonas,Serratia,Burkholderia,Rhizobium,Phyllobacterium,Paenibacillus,Bacillus,Rhodococcus,Curtobacterium and Arthrobacter,with Pseu- domonas,Phyllobacterium,Arthrobacter are dominant groups.The results showed the community structure and diversity of nitrogen-fixing bacteria in the rhizosphere soil of L.gmelinii in Arxan National Forest Park.

Key words: Arxan;Larixgmelinii;Nitrogen-fixing bacteria;16S rDNA

中图分类号: 

  • S714.3