北方农业学报 ›› 2019, Vol. 47 ›› Issue (2): 104-110.doi: 10.3969/j.issn.2096-1197.2019.02.18

• 著者文摘 • 上一篇    下一篇

宿晓红葡萄色素提取及稳定性研究

罗桂杰[1];刘博[1];陈芬[1]   

  1. [1]江苏省农业科学院宿迁农业科学研究所,江苏宿迁223800
  • 收稿日期:2019-02-26 出版日期:2019-02-19 发布日期:2019-08-19
  • 作者简介:罗桂杰(1981—),女,副研究员,硕士,主要从事林木、果树、花卉的研究工作。
  • 基金资助:
    2016年宿迁市科技支撑计划项目(L201601).

Study on extraction technology and the stability of natural pigment from Suxiaohong grape

LUO Guijie[1];LIU Bo[1];CHEN Fen[1]   

  1. [1]Suqian Institute of Agricultural Sciences,Jiangsu Academy of Agricultural Sciences,Suqian 223800,China
  • Received:2019-02-26 Online:2019-02-19 Published:2019-08-19

摘要: 为了探讨宿晓红葡萄色素提取工艺的优选方法及影响宿晓红葡萄色素稳定性因素,试验采用化学溶剂法提取宿晓红葡萄色素,进行正交试验,优选提取工艺。试验探明最佳提取条件为:乙醇浓度为70%,浸提温度为80℃,浸提时间为60min,料液比为1∶15。同时,对宿晓红葡萄色素耐光性、耐氧化还原性、耐酸碱性、耐糖等条件下的稳定性进行了研究,结果表明:宿晓红葡萄色素在pH值1.0~4.0的酸性环境下稳定;该色素对光照敏感,长时间照射会导致色素快速降解;在试验中加入低浓度Na2SO3、苯甲酸钠、山梨酸钾有利于色素稳定性的保持;高浓度的柠檬酸、Zn2+对色素有一定的护色作用;Ca^2+<1.0mol/L、蔗糖浓度<20%具有较好的辅色效果,大于这个范围辅色作用减弱。

关键词: 宿晓红葡萄;色素;提取;稳定性

Abstract: This experiment explored the preferred method for the extraction of Suxiaohong grape pigment and the factors affecting pigment stability.The rape pigment was extracted with varying concentrations of ethanol,extraction period and temperature,and an orthogonal test was carried out to optimize the extraction process.The best extraction conditions were found to be: ethanol concentration,70%;extraction temperature,70℃;extraction time,60 minutes;and material to liquid ratio,1∶15.The factors influencing the stability of Suxiaohong grape pigment studied were light tolerance,redox resistance and acid and alkali resistance.The study showed that the pigment from Suxiaohong grape is stable in a strong acid environment of pH 1.0-4.0;the pigment is sensitive to light and will rapidly degrade under long-term exposure. Pigment stability can be maintained by adding a low concentration of Na2SO3,sodium benzoate and potassium sorbate preservative which has a certain color protection effect on pigments with high a concentration of citric acid and zinc ions.A better secondary color effect was achieved by adding Ca^2+ at a concentration of<1.0 mol/L and sucrose at a concentration of<20% to the pigment.

Key words: Suxiaohong grape;Pigment;Extraction;Stability

中图分类号: 

  • S663.1