Abstract: At different temperatures and pharmaceutical treatment, we studied germination characteristics of 5 Koeleria cristata （L.） Pets population＇s seeds from three regions. The results showed that： （1）In 25 ℃ constant temperature, the average germination rate of Koeleria cristata （L.） Pers seeds are 88.16%,total germination time is 8d; In varied temperature and 15 ℃ constant temperature,the average germination rate are 80% and 50.8%, total germination time is 13d and 14d, low temperature significantly reduced germination rate and delayed the germination process of Koeleria cristata （L.） Pers seeds. （2）In 25 ℃ constant temperature, Koeleria cristata （L.） Pets seeds from Da Qing Shan region had highest germination rate, is up to 94%; In varied temperature, Koeleria cristata （L.） Pers seeds from Zhao He region had highest germination rate,is up to 85.20 %. （3） after soaking the seeds in gibberellic acid, in 15 ℃ constant temperature all seeds germination rate generally increased from 50% to 87% and the total germination time shortened by 8-10d;But in 25 ℃ constant temperature germination rate of k seeds from Da Qing Shah region decreased to 84%, from the Xilin Go LE Grassland, Zhao He grass region have been increased.