北方农业学报 ›› 2012, Vol. ›› Issue (2): 48-48.

• 试验研究 • 上一篇    下一篇

兰花外植体消毒试验初探

丁丽[1] 唐虹[2] 吴家丽[2] 李怀情[2] 鲍菊[2]   

  1. [1]毕节职业技术学院,贵州毕节551700 [2]安顺市农业科学研究所,贵州安顺561000
  • 出版日期:2012-04-20 发布日期:2012-04-20
  • 通讯作者: 丁丽
  • 作者简介:丁丽(1970-),女,贵州毕节人,高级农艺师,副教授,学士,从事土肥、农业技术推广、农业科研和教学工作
  • 基金资助:
    安顺市农业科学研究所所级基金项目

Study on the Sterilization of Orchid Explants

DING Li(Bijie Vocational Technical College,Bijie 551700,China)   

  • Online:2012-04-20 Published:2012-04-20

摘要: 以兰花新芽为组培材料,进行外植体消毒试验。结果表明:外植体用70%酒精浸30s后,于10%次氯酸钠溶液中消毒15~20min或0.5%升汞溶液中消毒5~8min,能有效降低外植体污染率,提高成活率。

Abstract: The explant disinfection experiment adopts orchid buds as the tissue culture materials.The results indicate that: after the explant is soaked in 70% alcohol for 30s,and disinfected in 10% sodium hypochlorite solution for 15 ~ 20 min or in 0.5% HgCl2 solution for 5 ~ 8 min,the pollution rate of explant can be effectively reduced,and the survival rate can be increased.

中图分类号: 

  • S682.31