北方农业学报 ›› 2019, Vol. 47 ›› Issue (5): 82-85.doi: 10.3969/j.issn.2096-1197.2019.05.15

• 园艺·农产品加工 • 上一篇    下一篇

葱兰愈伤组织诱导及植株再生

熊琴1,2, 付素静1, 鲁万贵3   

  1. 1.铜仁学院 农林工程与规划学院,贵州 铜仁 554300;
    2.铜仁市万山区鱼塘侗族苗族乡初级中学,贵州 铜仁 554300;
    3.铜仁市园林局,贵州 铜仁 554300
  • 收稿日期:2019-08-28 出版日期:2019-10-20 发布日期:2019-12-11
  • 通讯作者: 付素静(1980—),女,教授,硕士,主要从事园林植物栽培与应用的研究工作。
  • 作者简介:熊琴(1991—),女,二级教师,学士,主要从事初中生物的教学工作。

Callus induction and plant regeneration of Zephyranthes candida

XIONG Qin1,2, FU Sujing1, LU Wangui3   

  1. 1.College of Agroforestry Engineering and Planning,Tongren University,Tongren 554300,China;
    2.Yutang Dong and Miao Nationalities Township Junior Secondary School,Tongren 554300,China;
    3.Tongren Gardens Bureau,Tongren 554300,China
  • Received:2019-08-28 Online:2019-10-20 Published:2019-12-11

摘要: 为了给园林绿化提供更多的优质种苗,以葱兰种子作为外植体进行组织培养。结果表明:低浓度的2,4-D单独使用对葱兰种子愈伤组织的诱导效果不明显,MS+2,4-D 0.2 mg/L处理葱兰种子愈伤组织诱导率仅为3.3%;复合使用6-BA后葱兰种子愈伤组织和丛生芽的诱导率明显增加,MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L处理葱兰种子愈伤组织诱导率为90.0%,MS+6-BA 1.0 mg/L+NAA 1.0 mg/L处理葱兰丛生芽诱导率为66.7%。结论为诱导葱兰种子愈伤组织最佳培养基是MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L,诱导葱兰丛生芽的最佳培养基是MS+6-BA 1.0 mg/L+NAA 1.0 mg/L。

关键词: 葱兰, 组织培养, 愈伤组织, 丛生芽

Abstract: The seeds of Zephyranthes candida as the explants were cultured in vitro for providing a higher quality seedling to landscaping.The results showed that the single addition of a low concentration of 2,4-D to the medium was ineffective to induce callus,the callus induction rate was only 3.3% when treated with MS+2,4-d 0.2mg /L,while 2,4-D together with 6-BA in the medium was promoting induction of callus and multiple shoots obviously.The callus induction rate was 90% on medium of MS containing 0.4 mg/L 2,4-D and 0.2 mg/L 6-BA,while the multiple shoot induction rate was 66.7% on medium of MS containing 1 mg/L 6-BA and 1 mg/L NAA.The callus induction optimal media was MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L,and the multiple shoot induction optimal media was MS+6-BA 1 mg/L+NAA 1 mg/L.

Key words: Zephyranthes candida, Tissue culture, Callus, Multiple shoot

中图分类号: 

  • S682.31