Abstract: Objective The purpose of this experiment is to induce calli with stem sections and leaves of dill as explants,and lay the foundation of dill regeneration system establishment.Methods The dill seeds were incubated in warm water containing 0.1 mg/L gibberellic acid（GA） in shaker for 1-2 h,then planted in pots.The stem sections and leaves were separated from the cultivated plants,and dipped into mercuric chloride to sterilize for 4 min and 3 min,respectively.Afterwards,these explants were settled on culture medium for callus induction.Results The suitable culture mediums for callus induction from stem section and leaf were MS ＋sucrose ＋agar ＋2.0mg/L6-BA ＋0.5mg/L NAA and MS ＋sucrose ＋agar ＋2.0mg/L6-BA ＋0.7mg/L NAA,respectively. Conclusion In this paper,we have established the callus induction system with stem sections and leaves of dill successfully.