百里香乙醇提取液对黄瓜枯萎病的抑菌作用

doi:10.12190/j.issn.2096-1197.2020.01.17

北方农业学报 ›› 2020, Vol. 48 ›› Issue (1): 87-92.doi: 10.12190/j.issn.2096-1197.2020.01.17

百里香乙醇提取液对黄瓜枯萎病的抑菌作用

张禄¹, 康利平², 刘佃林²

1.内蒙古广播电视大学,内蒙古呼和浩特 010051;
2.呼和浩特职业学院,内蒙古呼和浩特 010051

收稿日期:2019-12-10 出版日期:2020-01-19 发布日期:2020-05-08
通讯作者: 康利平（1977—）,女,教授,博士,主要从事蔬菜栽培生理及野生植物资源开发应用的研究工作。
作者简介:张禄（1977-）,男,副教授,硕士,主要从事高寒地区植物病理学的研究工作。
基金资助:
内蒙古自治区高等学校科学技术研究项目（NJZY16364）

Bacteriostasis effect of ethanol extracting solution of thyme on pathogen of cucumber fusarium wilt

ZHANG Lu¹, KANG Liping², LIU Dianlin²

1.Inner Mongolia Radio and TV University,Hohhot 010051,China;
2.Hohhot Vocational College,Hohhot 010051,China

Received:2019-12-10 Online:2020-01-19 Published:2020-05-08

摘要/Abstract

摘要： 【目的】揭示百里香乙醇提取液对黄瓜枯萎病病菌的抑制作用。【方法】采用乙醇萃取,利用索氏抽提装置对百里香（Thymus mongolicus Ronn）茎叶鲜样、茎叶干样、鲜花进行提取,然后将提取液稀释不同倍数,并添加到PDA培养基中,进行黄瓜枯萎病病菌的培养试验,同时对黄瓜枯萎病病菌生长情况及抑菌效果进行观测。【结果】黄瓜枯萎病病菌在添加百里香乙醇提取液培养基上的生长速率降低;百里香各部位乙醇提取液对黄瓜枯萎病病菌均具有抑制作用,且抑菌率因部位和稀释浓度不同而不同,在培养144 h后,百里香茎叶鲜样乙醇提取液稀释2倍的处理抑菌率最高,达45.51%,抑菌效果随浓度的增加而增强。【结论】百里香乙醇提取液对黄瓜枯萎病病菌的生长具有抑制作用,且具有开发成黄瓜枯萎病抑菌剂的潜力。

关键词: 百里香, 乙醇提取液, 黄瓜枯萎病, 抑菌作用

Abstract: 【Objective】 To reveal the inhibitory effect of ethanol extracting solution of thyme on pathogen of cucumber fusarium wilt. 【Methods】The ethanol extraction method,the use of soxhlet apparatus of thyme （Thymus mongolicmus Ronn） stem and leaf fresh samples,stem and leaf dry samples,flower fresh samples,then diluted extract solution to different concentration,and added to the PDA medium,cucumber blight pathogen cultivation experiment was carried out. At the same time the cucumber fusarium wilt fungus growth situation and the bacteriostatic effects were observed. 【Results】The growth rate of pathogen of cucumber fusarium wilt on the medium of ethanol extracting solution with thyme was decreased. The ethanol extracting solution from all parts of thyme had inhibitory effect on the pathogen of cucumber fusarium wilt,and the bacteriostasis rate was different depending on the location and dilution concentration. After 144 h of culture,the bacteriostasis rate of the ethanol extracting solution which was diluted twice of the fresh sample of thyme stem and leaf,was the highest,reaching 45.51%,and the bacteriostasis effect was enhanced with the increase of the concentration. 【Conclusion】The ethanol extracting solution of thyme has the inhibitory effect on the growth of the pathogen of cucumber fusarium wilt,and has the potential to develop into the bacteriostasis agent of cucumber fusarium wilt.

Key words: Thyme, Ethanol extracting solution, Cucumber fusarium wilt, Bacteriostasis effect

中图分类号:

S436.421.13

张禄, 康利平, 刘佃林. 百里香乙醇提取液对黄瓜枯萎病的抑菌作用[J]. 北方农业学报, 2020, 48(1): 87-92.

ZHANG Lu, KANG Liping, LIU Dianlin. Bacteriostasis effect of ethanol extracting solution of thyme on pathogen of cucumber fusarium wilt[J]. Journal of Northern Agriculture, 2020, 48(1): 87-92.

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百里香乙醇提取液对黄瓜枯萎病的抑菌作用

Bacteriostasis effect of ethanol extracting solution of thyme on pathogen of cucumber fusarium wilt

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