北方农业学报 ›› 2016, Vol. 44 ›› Issue (2): 18-18.
赵彦[1,2,3];陈雪英[1];云锦凤[1,2,3];刘湘萍[4]
ZHAO Yan, CHEN Xueying, YUN Jinfeng, LIU Xiangping ( 1.College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Hohhot 010018, China ; 2.Engineering Center of Technical Research on Cultivation and Propagation of Grass Varieties, Hohhot 010018, China;3.Key Laboratory of Grassland Resources (IMAU) ,Ministry of Education,Hohhot 010018 China;4.Institute of vegetables Research,Inner Mongolia Academy of Agriculture and Animal Husbandry sciences,Hohhot 010018,China)
摘要: 试验通过对蒙古冰草不同部位的离体培养,筛选出了茎尖为诱导愈伤的适宜外植体,并通过MS基本培养基添加不同激素配比试验,建立了一套以蒙古冰草茎尖为外植体的再生体系,其中最适愈伤诱导培养基为MS+1.5 mg/L 2,4-D+0.1 mg/L 6-BA,诱导率达到84.9%;最适分化培养基MS+2.0 mg/L 6-BA+0.5 mg/L NAA,分化率为53.2%;生根培养基MS+0.5 mg/L NAA,生根率为100%。这是首次建立以蒙古冰草营养体为外植体的再生体系,为蒙古冰草的遗传转化奠定了基础。
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