北方农业学报 ›› 2016, Vol. 44 ›› Issue (2): 18-18.

• 研究报告 • 上一篇    下一篇

蒙古冰草茎尖愈伤组织及其再生植株诱导

赵彦[1,2,3];陈雪英[1];云锦凤[1,2,3];刘湘萍[4]   

  1. [1]内蒙古农业大学生态环境学院,内蒙古呼和浩特010018 [2]内蒙古自治区草品种育繁工程技术研究中心,内蒙古呼和浩特010018 [3]草地资源教育部重点实验室,内蒙古呼和浩特010018 [4]内蒙古农牧业科学院蔬菜研究所,内蒙古呼和浩特010031
  • 出版日期:2016-04-20 发布日期:2016-04-20
  • 通讯作者: 赵彦
  • 作者简介:赵彦(1976-),女,内蒙古呼和浩特人,博士,主要从事牧草遗传与育种研究。
  • 基金资助:
    国家自然科学基金(31260578);(31160479); 内蒙古草业重大专项“优质牧草种质资源创新与产业化示范”(20120245)

Induction of callus and plant regeneration from shoot tip in Agropyron mongolicum Keng

ZHAO Yan, CHEN Xueying, YUN Jinfeng, LIU Xiangping ( 1.College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Hohhot 010018, China ; 2.Engineering Center of Technical Research on Cultivation and Propagation of Grass Varieties, Hohhot 010018, China;3.Key Laboratory of Grassland Resources (IMAU) ,Ministry of Education,Hohhot 010018 China;4.Institute of vegetables Research,Inner Mongolia Academy of Agriculture and Animal Husbandry sciences,Hohhot 010018,China)   

  • Online:2016-04-20 Published:2016-04-20

摘要: 试验通过对蒙古冰草不同部位的离体培养,筛选出了茎尖为诱导愈伤的适宜外植体,并通过MS基本培养基添加不同激素配比试验,建立了一套以蒙古冰草茎尖为外植体的再生体系,其中最适愈伤诱导培养基为MS+1.5 mg/L 2,4-D+0.1 mg/L 6-BA,诱导率达到84.9%;最适分化培养基MS+2.0 mg/L 6-BA+0.5 mg/L NAA,分化率为53.2%;生根培养基MS+0.5 mg/L NAA,生根率为100%。这是首次建立以蒙古冰草营养体为外植体的再生体系,为蒙古冰草的遗传转化奠定了基础。

Abstract: The different parts of Mongolia wheatgrass were in vitro cultured in this experiment, and shoot tips screened out was suitable explants for induced callus. We also used basic medium MS supplemented with different hormones to set up a regeneration system of Agropyron Mongolian shoot tips as explants,and the results showed that the optimal callus induction medium is MS +1.5 mg/L 2,4-D+0.1 mg/L 6-BA, the inducted rate reached 84.9%;the optimum differentiation medium MS+6-BA 2.0 mg/L+NAA 0.5 mg/L,the differentiation rate was 53.2%;the rooting medium MS+NAA 0.5 mg/L,the rooting rate was 100%.This is the first established regeneration system of the Mongolia wheatgrass vegetative as explants,and laid the foundation for genetic transformation of Mongolia wheatgrass.

中图分类号: 

  • S812