北方农业学报 ›› 2017, Vol. 45 ›› Issue (5): 12-12.

• 育种·栽培·分子生物学 • 上一篇    下一篇

产淀粉酶益生菌的筛选及鉴定

连海飞;王晓冬;王德宝;梁俊芳;王乐;王丽梅;纳钦   

  1. 内蒙古农牧业科学院,内蒙古呼和浩特010031
  • 出版日期:2017-10-20 发布日期:2017-10-20
  • 通讯作者: 连海飞
  • 作者简介:连海飞(1983-),男,助理研究员,学士,主要从事质量分析与农药残留检测的研究工作. 通讯作者:王晓冬(1963-),男,高级实验师,学士,主要从事食品加工的研究工作.
  • 基金资助:
    内蒙古农牧业科学院青年创新基金项目(2017QNJJM10)

Screening and identification of bacteria producing amylase

LIAN Haifei, WANG Xiaodong, WANG Debao, LIAO Junfang, WANG Le, WANG Limei, NA Qin (Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010010, China)   

  • Online:2017-10-20 Published:2017-10-20

摘要: 应用微生物学传统分离、培养、筛选的方法,对14株菌株进行了活化、保藏,同时对试验菌株进行了染色镜检、生化鉴定,进行了淀粉酶菌株的初筛、复筛,目的是获得产淀粉酶较强的菌株,并对其酶活力进行鉴定。结果显示:菌株HZ9、HZ23为乳酸菌,且分解淀粉的能力相对较弱;HZ4、HZ5为芽孢杆菌,分解淀粉能力较强,该菌株在37℃时生长旺盛,代谢能力强,繁殖速度较快;其余菌株对淀粉无分解能力。因此,通过筛选鉴定获得产淀粉酶且活力较强的菌株为HZ4、HZ5。

Abstract: Appling microbiology in traditional isolation and screening methods,14 strains were activated and preserved.And the test strains were stained,biochemical identified and the amylase strain screened firstly and screened secondly.The purpose of this test was to screen the strains producing amylase stronger,and the enzyme activity were identified.The results showed that among strains codenamed HZ9,HZ23 were lactic acid bacteria.Their capability of breaking down starch was relatively weak.But the HZ4 and HZ5 were bacillus.Their capability of breaking down starch was strong.The strains growth vigorously,metabolize strongly,and produce quickly at 37 ℃.The other strains did not have the degrading ability on starch.Thus,by screening to produce amylase and strong active strain were HZ4 and HZ5.

中图分类号: 

  • Q814.2