北方农业学报 ›› 2020, Vol. 48 ›› Issue (1): 98-101.doi: 10.12190/j.issn.2096-1197.2020.01.19

• 园艺 • 上一篇    下一篇

内蒙古鄂尔多斯市野生羊肚菌ITS初步鉴定

庞杰1, 2, 刘海3, 康立茹1, 2, 于传宗1, 2, 王海燕1, 2, 李亚娇1, 2, 孙国琴1, 2   

  1. 1.内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031;
    2.食用菌内蒙古自治区工程研究中心,内蒙古 呼和浩特 010031;
    3.鄂托克旗草原工作站,内蒙古 乌兰镇 016100
  • 收稿日期:2020-01-17 出版日期:2020-01-19 发布日期:2020-05-08
  • 作者简介:庞杰(1986-),男,副研究员,博士,主要从事食用菌种质资源收集与创新的研究工作。
  • 基金资助:
    内蒙古自治区农牧业科学院青年创新基金项目(2015QNJJN01)

ITS identification of wild Morchella in Ordos of Inner Mongolia

PANG Jie1, 2, LIU Hai3, KANG Liru1, 2, YU Chuanzong1, 2, WANG Haiyan1, 2, LI Yajiao1, 2, SUN Guoqin1, 2   

  1. 1.Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences,Hohhot 010031,China;;
    2.Engineering Research Center of Edible Mushroom Inner Mongolia Autonomous Region,Hohhot 010031,China;
    3.Otog Banner Grassland Work Station,Wulan Town 016100,China
  • Received:2020-01-17 Online:2020-01-19 Published:2020-05-08

摘要: 【目的】探明内蒙古鄂尔多斯市野生羊肚菌的分布和种类。【方法】 采用野生食用菌常用的鉴定方式——ITS测序对收集到的野生羊肚菌进行鉴定。【结果】 鄂尔多斯市收集到的羊肚菌ITS序列长度为1 185 bp,其中CG为54.43%,NCBI数据库序列号为MN872380。【结论】 鄂尔多斯市野生羊肚菌为黄色羊肚菌类,但其拉丁学名尚不能通过单一的ITS确认,有待进一步通过LSU、EFla、RPB1和RPB2等多基因联合分析确定。

关键词: 羊肚菌, 鄂尔多斯, ITS鉴定

Abstract: 【Objective】In order to find out the distribution and species of wild Morchella in Ordos City of Inner Mongolia. 【Methods】 ITS sequencing was used to identify the wild Morchella collected in Ordos area.【Results】The results showed that its sequence length of Morchella collected in Ordos area was 1 185 bp,of which CG was 54.43% and NCBI database was MN872380.【Conclusion】Wild Morchella in Ordos area could be identified as yellow Morchella Morels,but its Latin name cannot be confirmed by itself alone,which needs to be further confirmed by LSU,EFla,RPB1 and RPB2.

Key words: Morchella, Ordos, ITS identify

中图分类号: 

  • S646.7