关键词: 橡胶草;GGPPS启动子;转基因拟南芥;缺失片段

Abstract: Based on the functional analysis of the GGPPS gene in the rubber grass and the cloned GGPPS gene promoter,the cis-acting element of the GGPPS gene promoter functional region was studied by the 5'deletion analysis method.The PCAMBIA1301-ProTkGGPPS-GUS was constructed,and each of the deletion fragment plant expression vectors were confirmed by Agrobacterium-mediated transient transformation of onion inner epidermal cells.The results showed that except for the TAGGPPS promoter,the full-length activity was slightly weaker,and the other deletion fragments were highly active.It can efficiently drive the expression of downstream GUS genes,and using the genetic transformation model plant Arabidopsis thaliana the transgenic seedlings of each vector were successfully obtained.These results lay the foundation for the subsequent study of the biological function of the cis-acting element on the promoter.

Key words: Taraxacum kok-saghyz Rodin;GGPPS promoter;Transgenic Arabidopsis thaliana;Deletion fragment