新疆橡胶草胚性悬浮细胞体系的建立

doi:10.3969/j.issn.2096-1197.2018.06.07

北方农业学报 ›› 2018, Vol. 46 ›› Issue (6): 32-37.doi: 10.3969/j.issn.2096-1197.2018.06.07

新疆橡胶草胚性悬浮细胞体系的建立

朱乐乐[1];杨露露[1];任汝豪[1];杨青哲[1];张慧艳[1];闫洁[1]

[1]石河子大学生命科学学院,新疆石河子832000

收稿日期:2018-11-01 出版日期:2018-08-19 发布日期:2019-08-19
作者简介:朱乐乐(1996—),女,本科生,专业为植物生长发育代谢调控;通讯作者:闫洁(1969—),女,副教授,博士,硕士生导师,主要从事植物生长发育代谢调控的研究工作。
基金资助:
国家自然科学基金项目(31360060)。

Establishment of embryonic suspension cell system of Taraxacum kok-saghyz Rodin in Xinjiang

ZHU Lele[1];YANG Lulu[1];REN Ruhao[1];YANG Qinzhe[1];ZHANG Huiyan[1];YAN Jie[1]

[1]College of Life Science,Shihezi University,Shihezi 832000,China

Received:2018-11-01 Online:2018-08-19 Published:2019-08-19

摘要/Abstract

摘要： 为了建立良好的橡胶草(Taraxacumkok-saghyz Rodin)悬浮细胞培养体系,以橡胶草无菌苗为材料,探究橡胶草悬浮细胞生长变化过程,结果表明:在50mLMS液体培养基中最适接种量为0.6g,最佳继代时间是12d,可以获得稳定的胚性悬浮细胞体系,再生率最高达到87.5%,为橡胶草的遗传再生体系奠定基础。

关键词: 橡胶草;悬浮培养;植株再生

Abstract: In order to establish a good suspension cell culture system of Taraxacum kok-saghyz Rodin,this study took the aseptic seedling of TKS as the material,to explore the growth process of TKS suspension cells.The results showed that the optimum inoculation amount of embryogenic callus is 0.6 g in 50 mL MS liquid medium,and the optimum time of subculture is 12 days.The stable system of the embryonic suspension cells can be obtained,and the highest regeneration rate could be up to 87.5%.These results lay the foundation for the genetic regeneration system of TKS.

Key words: Taraxacum kok-saghyz Rodin;Suspension culture;Plant regeneration

中图分类号:

Q949.97

朱乐乐;杨露露;任汝豪;杨青哲;张慧艳;闫洁. 新疆橡胶草胚性悬浮细胞体系的建立[J]. 北方农业学报, 2018, 46(6): 32-37.

ZHU Lele;YANG Lulu;REN Ruhao;YANG Qinzhe;ZHANG Huiyan;YAN Jie(College of Life Science,Shihezi University,Shihezi 832000,China). Establishment of embryonic suspension cell system of Taraxacum kok-saghyz Rodin in Xinjiang[J]. Journal of Northern Agriculture, 2018, 46(6): 32-37.

新疆橡胶草胚性悬浮细胞体系的建立

Establishment of embryonic suspension cell system of Taraxacum kok-saghyz Rodin in Xinjiang

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