灯笼红香瓜枯萎病病原菌分离鉴定及药剂防治研究

doi:10.12190/j.issn.2096-1197.2022.03.08

北方农业学报 ›› 2022, Vol. 50 ›› Issue (3): 60-66.doi: 10.12190/j.issn.2096-1197.2022.03.08

灯笼红香瓜枯萎病病原菌分离鉴定及药剂防治研究

白锦江¹, 赵健¹, 刘颖², 郑红丽¹, 赵明敏¹

1.内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特 010011;
2.鄂尔多斯生态环境职业学院,内蒙古东胜 012000

收稿日期:2022-04-02 修回日期:2022-05-10 出版日期:2022-06-20 发布日期:2022-08-09
通讯作者: 赵明敏（1975—）,女,教授,博士,主要从事植物病毒学的研究工作。
作者简介:白锦江（1994—）,男,硕士研究生,研究方向为植物病害综合防治。
基金资助:
内蒙古农业大学高层次人才引进科研启动项目（NDGCC2016-23）

Isolation and identification of pathogen causing Fusarium wilt in Denglonghong muskmelon and study on plant derived fungicide control

BAI Jinjiang¹, ZHAO Jian¹, LIU Ying², ZHENG Hongli¹, ZHAO Mingmin¹

1. College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010011,China;
2. Ordos Vocational College of Eco-environment,Dongsheng 012000,China

Received:2022-04-02 Revised:2022-05-10 Online:2022-06-20 Published:2022-08-09

摘要/Abstract

摘要： 【目的】明确引起内蒙古巴彦淖尔市五原县灯笼红香瓜枯萎病的病原菌,探究植物免疫诱抗剂和植物源药剂对该病害的防治作用。【方法】利用组织分离法对灯笼红香瓜枯萎病病原菌进行分离,采用柯赫氏法则对其致病性进行分析,并利用盆栽法进行4种药剂（植物免疫诱抗剂JM1、JM2、JM3,植物源药剂蛇床子素）的室内药效测定。【结果】从表现枯萎症状的香瓜上分离的真菌,可以回接到香瓜幼苗并引起典型的香瓜枯萎病症状,将该菌株命名为TG2菌株。根据菌落、孢子特征、孢子形态及ITS序列检测,将其确定为尖孢镰刀菌（Fusarium oxysporum）。JM1、JM2、JM3及蛇床子素对灯笼红香瓜枯萎病均有一定的防治效果,分别为45.99%、61.43%、15.13%、65.28%。【结论】尖孢镰刀菌（Fusarium oxysporum）是引起五原县灯笼红香瓜枯萎病的病原菌;植物免疫诱抗剂JM2与植物源药剂蛇床子素的防治效果较佳。

关键词: 灯笼红香瓜, 枯萎病, 尖孢镰刀菌, 植物免疫诱抗剂, 植物源药剂

Abstract: 【Objective】To identify the pathogen causing Denglonghong muskmelon Fusarium wilt in Wuyuan County,Bayannur City,Inner Mongolia,and investigate the disease response to plant immune inducers and plant-derived fungicide.【Methods】Tissue isolation method was used to isolate the Fusarium wilt pathogen from Denglonghong muskmelon,and Koch′s rule was used to assess its pathogenicity. The in door efficacy of four drugs（plant immune inducers JM1,JM2,JM3,and plant-derived fungicide Osthol）was evaluated using potted plants.【Results】The fungi isolated from muskmelon with wilting symptoms could inoculate muskmelon seedlings and cause typical muskmelon wilt symptoms. The strain was named TG2. According to the colony,spore characteristics,spore morphology and ITS sequence,it was identified as Fusarium oxysporum. JM1,JM2,JM3,and Osthol each demonstrated a control effect on the Fusarium wilt of 45.99%,61.43%,15.13%,and 65.28%,respectively. 【Conclusion】The pathogen that Causes Denglonghong muskmelon Fusarium wilt in Wuyuan County was Fusarium oxysporum. Plant immune inducer JM2,and plant-derived fungicide Osthol had better control effect.

Key words: Denglonghong muskmelon, Fusarium wilt, Fusarium oxysporum, Plant immune inducer, Plant-derived fungicide

中图分类号:

S436.5

白锦江, 赵健, 刘颖, 郑红丽, 赵明敏. 灯笼红香瓜枯萎病病原菌分离鉴定及药剂防治研究[J]. 北方农业学报, 2022, 50(3): 60-66.

BAI Jinjiang, ZHAO Jian, LIU Ying, ZHENG Hongli, ZHAO Mingmin. Isolation and identification of pathogen causing Fusarium wilt in Denglonghong muskmelon and study on plant derived fungicide control[J]. Journal of Northern Agriculture, 2022, 50(3): 60-66.

参考文献

[1] SILVIA S M,BGNARESI P,SESTILI S,et al.Transcriptome analysis of the melon-Fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants[J].Frontiers in Plant Science,2017,8:362.
[2] 邬佳.在希望的田野上[N].巴彦淖尔日报(汉),2022-05-06(005).
[3] 郭果枝,崔瑛,武英,等.香瓜品种灯笼红的大棚栽培技术[J].内蒙古农业科技,2014,42(3):115.
[4] BERROCAL-LOBO M,MOLINA A.Arabidopsis defense response against Fusarium oxysporum[J].Trends in Plant Science,2008,13(3):145-150.
[5] KISTLER H C,ALABOUVETTE C,BAAYEN R P,et al.Systematic numbering of vegetative compatibility groups in the plant pathogenic fungus Fusarium oxysporum[J].Phytopathology,1998,88(1):30-32.
[6] GORDON T R,MARTYN R D.The evolutionary biology of Fusarium oxysporum[J].Annual Review of Phytopathology,1997,35:111-128.
[7] EDEL-HERMANN V,LECOMTE C.Current status of Fusarium oxysporum Formae speciales and races[J].Phytopathology,2019,109(4):512-530.
[8] ABBAS H K.Characterization and Biological Activity of Two New Mycotoxins Produced by Species of Fusarium[M].So Paulo:University of Minnesota,1987.
[9] WILEY A W,SEARLE C,Pegg K G,et al.Micropropagated bananas are more susceptible to Fusarium wilt than plants grown from conventional material[J].Australian Journal of Agricultural Research,1998,49(7):1133-1140.
[10] MICHIELSE C B,REP M.Pathogen profile update:Fusarium oxysporum[J].Molecular Plant Pathology,2009,10(3):311-324.
[11] RAHMAN M Z,AHMAD K,SIDDIQUI Y,et al.First report of Fusarium wilt disease on watermelon caused by Fusarium oxysporum f. sp. niveum in Malaysia[J].Plant Disease,2021,105(12):41-69.
[12] 耿丽华,王建设,马建,等.一种甜瓜新症状病害的病原鉴定及其生物学特性[J].中国瓜菜,2021,34(3):15-20.
[13] 李家洲,洪妙珍,李玉婵,等.抗香蕉枯萎病病原菌中药材的筛选[J].中国南方果树,2014,43(2):64-67.
[14] 吕云霞. 马铃薯、大豆等难治病害的中药学防治新策略[J].农业科技通讯,2009(10):185-186.
[15] ZHENG H L,CHEN Y,GUO Q,et al.Inhibitory effect of Osthole from Cnidium monnieri(L.)Cusson on Fusarium oxysporum,a common fungal pathogen of potato[J].Molecules,2021,26(13):3818.
[16] 周婧,高增贵,何秀玲,等.甜瓜枯萎病拮抗内生细菌筛选研究[J].北方园艺,2008(2):225-227.
[17] GWYNNE B J,GORDON T R,DAVIS R M.A new race of Fusarium oxysporum f. sp. melonis causing Fusarium wilt of muskmelon in the central valley of California[J].Plant Disease,1997,81(9):1095.
[18] 延涵,王素娜,盖晓彤,等.东北地区甜瓜枯萎病种群鉴定及多样性分析[C]//中国植物保护学会2016年学术年会论文集.成都:中国农业科学技术出版社,2016:368.
[19] 黄文枫,胡艳平,朱白婢.海南薄皮甜瓜枯萎病病原菌鉴定及抗病砧木的筛选[J].热带生物学报,2020,11(3):310-313.
[20] 苗丹,路妍,陈雅寒,等.植物免疫剂JM1号对马铃薯枯萎病菌的抑制作用研究[J].内蒙古农业大学学报(自然科学版),2019,40(3):92-96.
[21] MASI F,CHIANESE G,PETERLONGO F,et al.Phytochemical profile of Centevita ,a Centella asiatica leaves extract,and isolation of a new oleanane-type saponin[J].Fitoterapia,2022,158:105163.
[22] ZHANG P,AN Q,YI P,et al.Thermlanseedlines A-G,seven thermopsine-based alkaloids with antiviral and insecticidal activities from the seeds of Thermopsis lanceolata R. Br.[J].Fitoterapia,2022,158:105140.

灯笼红香瓜枯萎病病原菌分离鉴定及药剂防治研究

Isolation and identification of pathogen causing Fusarium wilt in Denglonghong muskmelon and study on plant derived fungicide control

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	赵远征, 彭静文, 侯伟峰, 牟英男, 路景诗, 白锦江, 徐利敏. 不同药剂对马铃薯枯萎病的田间防效分析[J]. 北方农业学报, 2022, 50(5): 82-86.
[2]	路景诗, 赵远征, 王东, 李晓杰, 彭静文, 张龙梅, 张晓明. 尖孢镰刀菌全基因组密码子偏好性及特征分析[J]. 北方农业学报, 2022, 50(4): 11-17.
[3]	张禄, 康利平, 刘佃林. 百里香乙醇提取液对黄瓜枯萎病的抑菌作用[J]. 北方农业学报, 2020, 48(1): 87-92.
[4]	扈顺, 高婧, 王勇, 王永, 席先梅, 张俊, 周广俊, 李艾兰. 西芹根腐病病原菌的分离与鉴定[J]. 北方农业学报, 2019, 47(5): 65-69.
[5]	王绎, 赵玲玲, 杨雪峰, 陈海军, 邵科, 刘洪林, 侯亚光. 6种杀菌剂对番茄枯萎病菌的毒力测定[J]. 北方农业学报, 2019, 47(3): 98-101.
[6]	徐利敏;侯亚光;贾瑞芳;张俊;郭景山;高润蕾. 杀菌剂对马铃薯枯萎病菌的毒力测定[J]. 北方农业学报, 2018, 46(2): 65-65.
[7]	苑琳;郑雅心;李冠华. 萎缩芽孢杆菌SF1对尖孢镰刀菌胡麻专化型抑菌效果研究[J]. 北方农业学报, 2017, 45(3): 65-65.
[8]	梁小平李碧霞严忠诚刘必成. 尖孢镰刀菌所致日光温室蔬菜病害的防治[J]. 北方农业学报, 2014, (6): 46-46.
[9]	杨蕊芝. 万寿菊枯萎病病原菌初步鉴定[J]. 北方农业学报, 2013, (3): 89-89.
[10]	王永陈磊云兴福李楠. 西芹根物质水浸提液二次层析物对黄瓜枯萎病菌的化感作用[J]. 北方农业学报, 2013, (2): 33-33.
[11]	王志强李树林. VA菌根抗黄瓜枯萎病菌株筛选[J]. 北方农业学报, 2007, (4): 35-35.
[12]	贾志荣刘林. 河套地区西瓜枯萎病的防治对策[J]. 北方农业学报, 2004, (F12): 188-188.
[13]	解素文刘志萍. 地膜西瓜套作红薯高效栽培技术[J]. 北方农业学报, 2002, (S2): 184-184.
[14]	水旭东李军进胡德具. 宁波市郊设施栽培蔬菜主要病虫的发生及防治技术[J]. 北方农业学报, 2001, (S2): 208-208.
[15]	胡翠娥马明陆雪梅. 西瓜“死秧”防治技术[J]. 北方农业学报, 2000, (S1): 90-90.