饲喂葛叶对豆天蛾幼虫生长性能及肠道菌群多样性的影响

doi:10.12190/j.issn.2096-1197.2023.01.13

北方农业学报 ›› 2023, Vol. 51 ›› Issue (1): 107-117.doi: 10.12190/j.issn.2096-1197.2023.01.13

• 资源与环境·农产品加工 • 上一篇下一篇

饲喂葛叶对豆天蛾幼虫生长性能及肠道菌群多样性的影响

谢洁微¹, 李雪玲¹, 施庆珊², 吴俊松³, 朱剑锋⁴, 李锐明¹, 胡文锋¹

1.华南农业大学食品学院,广东广州 510642;
2.广东省科学院微生物研究所,广东广州 510700;
3.广州崧源农业科技有限公司,广东广州 510640;
4.生物源生物技术（深圳）股份有限公司,广东深圳 518118

收稿日期:2022-11-29 出版日期:2023-02-20 发布日期:2023-05-22
通讯作者: 胡文锋（1964—）,男,副教授,博士,主要从事食品发酵工程、食用昆虫和食品加工副产物及农副产品的研究工作。
作者简介:谢洁微（1995—）,女,硕士研究生,研究方向为食用昆虫资源化利用、微生物发酵与应用。
基金资助:
华南农业大学葛根研究院校企合作项目（H21733）

Effects of Pueraria lobata leaf on growth performance and intestinal microflora diversity of Clanis bilineata tingtauica larvae

XIE Jiewei¹, LI Xueling¹, SHI Qingshan², WU Junsong³, ZHU Jianfeng⁴, LI Ruiming¹, HU Wenfeng¹

1. College of Food Science,South China Agricultural University,Guangzhou 510642,China;
2. Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510700,China;
3. Guangzhou Songyuan Agricultural Technology Co. Ltd.,Guangzhou 510640,China;
4. Bioforte Biotechonology（Shenzhen）Co. Ltd.,Shenzhen 518118,China

Received:2022-11-29 Online:2023-02-20 Published:2023-05-22

摘要/Abstract

摘要： 【目的】探究葛叶替代传统的大豆叶饲喂对豆天蛾（Clanis bilineata tingtauica）幼虫生长性能和肠道菌群多样性的影响。【方法】试验选择大小一致、健康水平接近的0日龄豆天蛾幼虫,分别用葛叶（试验组）和大豆叶（对照组）饲喂,每组设置3个重复,每个重复200头幼虫,对比分析幼虫生长性能、存活率、饲料转化率、料重比、营养成分、肠道菌群多样性等指标的差异。【结果】与大豆叶相比,饲喂葛叶的豆天蛾幼虫（25日龄）存活率（57.54%）和饲料转化率（0.24 g/g）更高、料重比（1.90 g/g）更低。饲喂葛叶的豆天蛾幼虫生长性能较好,葛叶组25日龄幼虫的平均体长（72.27 mm）、平均体宽（11.99 mm）和平均体重（7.34 g）均高于大豆叶组;幼虫（干重）粗蛋白（64.12%）、粗脂肪（18.15%）含量以及必需氨基酸占氨基酸总量比例（37.72%）均高于大豆叶组。16S rRNA基因测序结果表明,葛叶组幼虫中肠道菌群多样性高于大豆叶组,主要包括厚壁菌门和多种有益菌属。【结论】综合考虑幼虫品质和经济效益,用葛叶替代大豆叶可以提高豆天蛾幼虫的生长性能和肠道菌群多样性。

关键词: 豆天蛾幼虫, 葛叶, 大豆叶, 生长性能, 肠道菌群多样性

Abstract: 【Objective】To explore the effects of replacing the traditional soybean leaf with Pueraria lobata leaf on growth performance and intestinal microflora diversity of Clanis bilineata tingtauica larvae.【Methods】In this experiment,0-day-old larvae with the similar size and health level were fed with Pueraria lobata leaf（experimental group）and soybean leaf（control group）respectively. Each group was set up with 3 replicates and each replicate comprised 200 larvae. The differences in growth performance,survival rate,digested feed conversion efficiency,feed to gain ratio,nutritional composition and intestinal microflora diversity of larvae were compared and analyzed.【Results】Compared with soybean leaf,Pueraria lobata leaf fed larvae had higher survival rate（57.54%）,higher digested feed conversion efficiency（0.24 g/g）and lower feed to gain ratio（1.90 g/g）. Larvae in the group fed with Pueraria lobata leaf had better growth performance. The average body length（72.27 mm）,average body width（11.99 mm）and average body weight（7.34 g）of the 25-day-old Clanis bilineata tingtauica were all higher than those in the group fed with soybean leaf. Larvae（dry weight）fed with Pueraria lobata leaf had higher content of crude protein（64.12%）,crude fat（18.15%）and higher proportion of essential amino acids in total amino acids（37.72%）compared with those fed with soybean leaf. According to the 16S rRNA sequencing results,Clanis bilineata tingtauica larvae fed with Pueraria lobata leaf had more diverse intestinal microflora than larvae fed with soybean leaf,which was primarily made up of Firmicutes and several beneficial bacterial genus.【Conclusion】Considering the larval quality and economic benefits,the substitution of Pueraria lobata leaf for soybean leaf improved the growth performance and diversity of intestinal microflora of Clanis bilineata tingtauica larvae.

Key words: Clanis bilineata tingtauica larvae, Pueraria lobata leaf, Soybean leaf, Growth performance, Diversity of intestinal microflora

中图分类号:

S899.9

谢洁微, 李雪玲, 施庆珊, 吴俊松, 朱剑锋, 李锐明, 胡文锋. 饲喂葛叶对豆天蛾幼虫生长性能及肠道菌群多样性的影响[J]. 北方农业学报, 2023, 51(1): 107-117.

XIE Jiewei, LI Xueling, SHI Qingshan, WU Junsong, ZHU Jianfeng, LI Ruiming, HU Wenfeng. Effects of Pueraria lobata leaf on growth performance and intestinal microflora diversity of Clanis bilineata tingtauica larvae[J]. Journal of Northern Agriculture, 2023, 51(1): 107-117.

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饲喂葛叶对豆天蛾幼虫生长性能及肠道菌群多样性的影响

Effects of Pueraria lobata leaf on growth performance and intestinal microflora diversity of Clanis bilineata tingtauica larvae

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