关键词: 金莲花, 种子, 赤霉素浓度, 变温条件, 浸泡时间, 萌发活力

Abstract: 【Objective】To investigate the effects of different varied temperature conditions,gibberellin concentrations,and soaking times on the germination activity of Trollius chinensis Bunge seeds,and to provide reference for the screening of germination activity conditions and sowing of T. chinensis Bunge seeds.【Methods】The seeds of T. chinensis Bunge collected in 2021 as experimental materials were respectively soaked with different concentrations of gibberellin（0,100,250,500,800,1 000,1 500 mg/L） for 24 h and 48 h,and varied temperature conditions（15/5,20/10,25/15,25/20,30/20,30/25 ℃） were set to simulate field temperature changes for germination experiments. The germination time of the seeds of T. chinensis Bunge and the germination rate,germination potential,and germination index were analyzed under 25/20 ℃ varied temperature conditions in different gibberellin concentrations and soaking times and soaking for 24 h in different varied temperature conditions and gibberellin concentrations.【Results】The germination start time of T. chinensis Bunge seeds was shorter under the conditions of soaking in 250 mg/L gibberellin for 24 h and 48 h at 25/20 ℃,soaking in 250 mg/L gibberellin for 24 h at 30/25 ℃,and soaking in 800 and 1 000 mg/L gibberellin for 48 h at 30/25 ℃,all of which were 6 d. Under the varied temperature condition of 25/20 ℃,the germination rate,germination potential,and germination index of T. chinensis Bunge seeds soaked for 48 h in 500 and 800 mg/L gibberellin treatments were significantly higher than soaked for 24 h（P<0.05）;at the same soaking time,seeds treated with 250 and 500 mg/L gibberellin showed higher germination rate,germination potential,and germination index. Under soaking for 24 h and varied temperature conditions of 20/10,25/15 and 25/20 ℃,the germination rate,germination potential,and germination index of T. chinensis Bunge seeds treated with 250 mg/L gibberellin were significantly higher than those treated with other gibberellin concentrations（P<0.05）;under the treatment of 250 mg/L gibberellin,the germination rate,germination potential,and germination index of T. chinensis Bunge seeds at 25/20 ℃ were significantly higher than those under other varied temperature conditions（P<0.05）.【Conclusion】The sensitivity of germination of T. chinensis Bunge seeds is different in varied temperature conditions and gibberellin concentration treatments. Both excessively high and low temperature conditions,as well as treatment with gibberellin concentration,are not conducive to the seed germination of T. chinensis Bunge;under the conditions of 25/20 ℃,treating the seed of T. chinensis Bunge with 250 mg/L gibberellin for 24 h can better improve germination activity.

Key words: Trollius chinensis Bunge, Seed, Gibberellin concentrations, Varied temperature conditions, Soaking time, Germination activity