北方农业学报 ›› 2021, Vol. 49 ›› Issue (6): 90-97.doi: 10.12190/j.issn.2096-1197.2021.06.12

• 生理生化·植物保护 • 上一篇    下一篇

玉米种子萌发抗旱药剂的筛选

屈佳伟, 高聚林, 于晓芳, 孙继颖, 王志刚, 王振, 田磊   

  1. 内蒙古农业大学 农学院,内蒙古 呼和浩特 010019
  • 收稿日期:2021-11-05 出版日期:2021-12-20 发布日期:2022-02-09
  • 通讯作者: 高聚林(1964—),男,教授,博士,博士生导师,主要从事玉米栽培与生理生态的研究工作。
  • 作者简介:屈佳伟(1988—),女,讲师,博士,主要从事玉米生理生态的教学及研究工作。
  • 基金资助:
    内蒙古农业大学引进优秀博士第四层次项目(NDYB2016-29); 内蒙古自治区自然科学基金项目(2019BS03016); 内蒙古自治区科技兴蒙行动重点专项项目(KJXM2020001-06); 国家现代农业产业技术体系建设专项项目(CARS-02-63); 作物栽培与遗传改良重点实验室资助项目

Screening of drought-tolerance regulators for maize seed germination

QU Jiawei, GAO Julin, YU Xiaofang, SUN Jiying, WANG Zhigang, WANG Zhen, TIAN Lei   

  1. College of Agriculture,Inner Mongolia Agricultural University,Hohhot 010019,China
  • Received:2021-11-05 Online:2021-12-20 Published:2022-02-09

摘要: 【目的】 筛选种子萌发抗旱药剂,提高玉米种子的抗旱性。【方法】 试验用浓度为25%的聚乙二醇6000(PEG-6000)模拟干旱条件,选取14种药剂引发,每种药剂设3个浓度,以蒸馏水引发为对照1(CK1),以不引发为对照2(CK2),经过初筛和复筛选择对先玉335和科河8号种子萌发都具有抗旱作用的化控调节剂。【结果】 在干旱胁迫下,100 mg/L乙烯利和100 mg/L烯效唑对2个玉米品种引发,种子发芽率、发芽指数、活力指数、萌发抗旱指数等显著高于其他药剂引发(P<0.05)。100 mg/L乙烯利引发的发芽率较蒸馏水引发(CK1)增加了6.67个百分点(科河8号)和18.00个百分点(先玉335),较不引发(CK2)增加了28.00个百分点(科河8号)和70.67个百分点(先玉335);100 mg/L烯效唑引发的发芽率较蒸馏水引发(CK1)增加了4.00个百分点(科河8号)和16.00个百分点(先玉335),较不引发(CK2)增加了16.00个百分点(科河8号)和68.67个百分点(先玉335);各药剂对先玉335的作用强于科河8号。【结论】 100 mg/L乙烯利和100 mg/L烯效唑对提高干旱胁迫下先玉335和科河8号种子的萌发具有显著效果,对抗旱药剂的进一步研发具有应用价值。

关键词: 玉米, 种子, 抗旱药剂, 乙烯利, 烯效唑

Abstract: 【Objective】Drought-tolerance regulators are being tested for seed germination in order to improve maize seed drought resistance.【Methods】Drought conditions were simulated in the experiment using polyethylene glycol 6000 at a concentration of 25%. 14 different regulators were chosen with three concentrations each. Control 1(CK1)was distilled water,and control 2(CK2)was non-initiation. Drought tolerance agents were selected by primary and secondary screening on seed germination of both Xianyu 335 and Kehe No.8.【Results】The seed germination rate,germination index,vigor index,and germination drought resistance index of two maize varieties induced by 100 mg/L Ethephon and 100 mg/L Uniconazole were significantly higher than those induced by other regulators under drought stress(P<0.05). The germination rate of 100 mg/L Ethephon increased by 6.67 percentage points(Kehe No.8)and 18.00 percentage points(Xianyu 335) compared with that of distilled water initiator(CK1),and 28.00 percentage points(Kehe No.8)and 70.67 percentage points(Xianyu 335) compared with that of non-initiation(CK2). The germination rate of 100 mg/L Uniconazole increased by 4.00 percentage points(Kehe No.8)and 16.00 percentage points(Xianyu 335)compared with that of distilled water initiator(CK1),and increased by 16.00 percentage points(Kehe No.8)and 68.67 percentage points(Xianyu 335)compared with that of non-initiation(CK2). The effect of both regulators on Xianyu 335 was stronger than on Kehe No.8.【Conclusion】Under drought stress,100 mg/L Ethephon and 100 mg/L Uniconazole had significant effects on Xianyu 335 and Kehe No.8 seed germination. This would be useful in future drought-tolerance regulator research.

Key words: Maize, Seed, Drought-tolerance regulator, Ethephon, Uniconazole

中图分类号: 

  • S513