籼稻资源稻瘟病抗性基因的分子检测与抗性分析

doi:10.12190/j.issn.2096-1197.2025.02.01

摘要/Abstract

摘要： 【目的】探明5个抗稻瘟病基因（Pi2、Pib、Pita、Pigm、Pi9）在86份籼稻资源中的分布及不同基因型组合的抗性效应,为抗稻瘟病品种的选育及稻瘟病抗性基因的有效聚合提供理论依据。【方法】利用5对扩增条带清晰的功能标记,对86份黄淮稻区籼稻的稻瘟病抗性基因进行检测,并结合人工接种鉴定,明确5个抗性基因的分布和该地区中、高抗性的基因型组合。【结果】5个抗性基因Pi2、Pib、Pita、Pigm、Pi9在86份籼稻中的分布频率分别为98.80%、46.50%、55.80%、48.80%、1.20%。在86份供试材料中共检测出10种基因组合,分别为不含任何抗性基因、Pi2、Pi2+Pita、Pi2+Pib、Pi2+Pigm、Pi2+Pib+Pita、Pi2+Pi9+Pigm、Pi2+Pib+Pigm、Pi2+Pita+Pigm、Pi2+Pib+Pita+Pigm。接种鉴定为中抗以上的材料共有33份,占比38.37%;含有3种基因型Pi2+Pib+Pita、Pi2+Pita+Pigm、Pi2+Pib+Pita+Pigm的材料中,中抗以上材料占比分别为50%、80%和100%。【结论】Pi2在86份籼稻中的分布频率最高,为98.80%;抗性较好的3个基因型组合分别是Pi2+Pib+Pita、Pi2+Pita+Pigm和Pi2+Pib+Pita+Pigm。

关键词: 籼稻, 稻瘟病, 抗性基因, 抗性分析, 基因型

Abstract: 【Objective】To investigate the distribution of five rice blast resistance genes（Pi2,Pib,Pita,Pigm,Pi9） in 86 indica rice accessions and evaluate the resistance effects of different genotypic combinations,providing a theoretical basis for breeding rice blast-resistant varieties and effective pyramiding of resistance genes.【Methods】Using five pairs of functional markers with clear amplification bands,blast resistance genes in 86 indica rice accessions from the Huang-Huai rice region were detected,and combined with artificial inoculation assays,the distribution of five resistance genes and the genotypic combinations conferring moderate to high resistance in this region were identified.【Results】The distribution frequencies of the five resistance genes Pi2,Pib,Pita,Pigm and Pi9 in the 86 indica rice accessions were 98.80%,46.50%,55.80%,48.80%,and 1.20%,respectively. Ten genotypic combinations were identified among the 86 tested materials,no resistance genes,Pi2,Pi2+Pita,Pi2+Pib,Pi2+Pigm,Pi2+Pib+Pita,Pi2+Pi9+Pigm,Pi2+Pib+Pigm,Pi2+Pita+Pigm and Pi2+Pib+Pita+Pigm,respectively. Inoculation assays showed that 33 accessions exhibited moderate or higher resistance,accounting for 38.37%;among the materials with three genotypic combinations Pi2+Pib+Pita,Pi2+Pita+Pigm and Pi2+Pib+Pita+Pigm,the proportions of accessions with moderate or higher resistance were 50%,80% and 100%,respectively.【Conclusion】The Pi2 gene exhibits the highest distribution frequency（98.80%） among the 86 indica rice accessions;the three genotypic combinations conferring superior resistance are Pi2+Pib+Pita,Pi2+Pita+Pigm and Pi2+Pib+Pita+Pigm.

Key words: Indica rice, Rice blast, Resistance genes, Resistance analysis, Genotype

中图分类号:

S435.111.41

陈春, 李凡, 郭新亚, 王飞飞, 王磊, 陈卫军. 籼稻资源稻瘟病抗性基因的分子检测与抗性分析[J]. 北方农业学报, 2025, 53(2): 1-9.

CHEN Chun, LI Fan, GUO Xinya, WANG Feifei, WANG Lei, CHEN Weijun. Molecular detection and resistance analysis of rice blast resistance genes in indica rice accessions[J]. Journal of Northern Agriculture, 2025, 53(2): 1-9.

参考文献

[1] 桑世飞,王亚男,王君怡,等.9个抗稻瘟病基因在291份水稻种质资源中的分布及组合特征[J].河南农业科学,2022,51(12):19-27.
[2] ASHKANI S,YUSOP M R,SHABANIMOFRAD M,et al.Genetic analysis of resistance to rice blast:a study on the inheritance of resistance to the blast disease pathogen in an F₃ population of rice[J].Journal of Phytopathology,2015,163(4):300-309.
[3] LUKASIK E,TAKKEN F L.Standing strong,resistance proteins instigators of plant defence[J].Current Opinion in Plant Biology,2009,12(4):427-436.
[4] ASHKANI S,RAFII M Y,SHABANIMOFRAD M,et al.Molecular progress on the mapping and cloning of functional genes for blast disease in rice(Oryza sativa L.):current status and future considerations[J].Critical Reviews in Biotechnology,2016,36(2):353-367.
[5] SHARMA T R,RAI A K,GUPTA S K,et al.Rice blast management through host-plant resistance:retrospect and prospects[J].Agricultural Research,2012,1(1):37-52.
[6] 吴云雨,肖宁,余玲,等.长江下游粳稻稻瘟病广谱抗性基因组合模式分析[J].中国农业科学,2021,54(9):1881-1893.
[7] HITTALMANI S,PARCO A,MEW T V,et al.Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice[J].Theoretical and Applied Genetics,2000,100(7):1121-1128.
[8] WU Y Y,XIAO N,CHEN Y,et al.Comprehensive evaluation of resistance effects of pyramiding lines with different broad-spectrum resistance genes against Magnaporthe oryzae in rice(Oryza sativa L.)[J].Rice,2019,12(1):11.
[9] 储黄伟,程灿,牛付安,等.8个稻瘟病抗性基因在三系杂交粳稻亲本中的分布[J].上海农业学报,2018,34(1):8-13.
[10] 李刚,袁彩勇,曹奎荣,等.544份水稻种质稻瘟病抗性鉴定及抗性基因的分布研究[J].中国农业大学学报,2018,23(5):22-28.
[11] 汪文娟,周继勇,汪聪颖,等.八个抗稻瘟病基因在华南籼型杂交水稻中的分布[J].中国水稻科学,2017,31(3):299-306.
[12] 邹茜,董丽英,袁平荣,等.广谱持久抗病基因Pi40在云南高原粳稻的应用研究[J].西南农业学报,2016,29(7):1493-1498.
[13] 邹德堂,姜思达,赵宏伟,等.广谱抗性基因Pi9在黑龙江省水稻品种中的分布[J].东北农业大学学报,2016,47(7):1-8.
[14] 王生轩,李俊周,谢瑛,等.河南粳稻抗稻瘟病基因Pi9、Pita和Piz-t的分子检测[J].分子植物育种,2017,15(3):951-955.
[15] 李耀栋,张银霞,李金吉,等.宁夏水稻代表性种质及后代抗稻瘟病基因型分析[J].植物遗传资源学报,2019,20(2):321-334.
[16] 王丽丽,赵家铭,马作斌,等.辽宁地区水稻资源抗稻瘟病基因的检测分析[J].植物遗传资源学报,2017,18(2):325-339.
[17] JIANG J,WANG S.Identification of a 118 kb DNA fragment containing the locus of blast resistance gene Pi2(t) in rice[J].Molecular Genetics and Genomics,2002,268(2):249-252.
[18] QU S H,LIU G F,ZHOU B,et al.The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice[J].Genetics,2006,172(3):1901-1914.
[19] 时克,雷财林,程治军,等.稻瘟病抗性基因Pita和Pib在我国水稻主栽品种中的分布[J].植物遗传资源学报,2009,10(1):21-26.
[20] 王忠华,贾育林,吴殿星,等.水稻抗稻瘟病基因Pita的分子标记辅助选择[J].作物学报,2004,30(12):1259-1265.
[21] DENG Y W,ZHU X D,SHEN Y,et al.Genetic characterization and fine mapping of the blast resistance locus Pigm(t) tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety[J].Theoretical and Applied Genetics,2006,113(4):705-713.
[22] 张燕红,贾春平,文孝荣,等.新疆水稻主要育成品种13个稻瘟病的抗性基因分布[J].新疆农业科学,2017,54(9):1595-1605.