蓖麻全基因组WRKY基因家族的鉴定及表达分析

doi:10.12190/j.issn.2096-1197.2026.02.02

北方农业学报 ›› 2026, Vol. 54 ›› Issue (2): 11-24.doi: 10.12190/j.issn.2096-1197.2026.02.02

• 种质资源·分子生物学 • 上一篇下一篇

蓖麻全基因组WRKY基因家族的鉴定及表达分析

张春玲¹, 狄建军^2,3,4, 韩雯毓¹, 刘庆秀⁵, 王冬艳³, 曲毅鹏¹, 张斯琴⁵, 何智彪^1,6,7

1.通辽市农牧业科学院,内蒙古通辽 028015;
2.内蒙古自治区蓖麻育种与综合利用重点实验室/蓖麻育种国家民委重点实验室,内蒙古通辽 028000;
3.内蒙古民族大学,内蒙古通辽 028000;
4.内蒙古自治区高校蓖麻产业工程技术研究中心/内蒙古自治区蓖麻育种重点实验室,内蒙古通辽 028000;
5.通辽市科尔沁区农业技术推广中心,内蒙古通辽 028000;
6.通辽市农业技术推广中心,内蒙古通辽 028000;
7.通辽市园林管理局,内蒙古通辽 028000

收稿日期:2025-05-08 出版日期:2026-04-20 发布日期:2026-06-10
作者简介:张春玲（1997—）,女,硕士,主要从事蓖麻分子育种方面的研究工作。
基金资助:
中央引导地方科技发展专项（YDZX202021）; 通辽市科技计划项目“通辽市农作物新品种选育及示范推广” 通辽市财政支持项目“种业振兴及新品种研发”

Identification and expression analysis of the WRKY gene family in the whole genome of Ricinus communis

ZHANG Chunling¹, DI Jianjun^2,3,4, HAN Wenyu¹, LIU Qingxiu⁵, WANG Dongyan³, QU Yipeng¹, ZHANG Siqin⁵, HE Zhibiao^1,6,7

1. Tongliao Academy of Agriculture and Animal Husbandry Sciences, Tongliao 028015, China;
2. Inner Mongolia Key Laboratory of Castor Breeding and Comprehensive Utilization/Key Laboratory of Castor Breeding of the State Ethnic Affairs Commission, Tongliao 028000, China;
3. Inner Mongolia Minzu University, Tongliao 028000, China;
4. Inner Mongolia Castor Industrial Engineering Research Center of Universities/Inner Mongolia Key Laboratory of Castor Breeding, Tongliao 028000, China;
5. Horqin District Agricultural Technology Extension Center of Tongliao City, Tongliao 028000, China;
6. Tongliao City Agricultural Technology Extension Center, Tongliao 028000, China;
7. Tongliao Landscape Management Bureau, Tongliao 028000, China

Received:2025-05-08 Online:2026-04-20 Published:2026-06-10

摘要/Abstract

摘要： 【目的】鉴定蓖麻WRKY转录因子家族成员,为进一步探究蓖麻WRKY基因家族成员的功能、进化及分子育种奠定基础。【方法】运用生物信息学方法对蓖麻WRKY转录因子家族成员蛋白质的理化性质、二级结构、保守基序、系统进化树、基因表达差异及启动子顺式作用元件进行分析。【结果】蓖麻WRKY基因家族中共鉴定出58个基因。理化性质和二级结构分析显示,蓖麻WRKY转录因子家族大多数为碱性蛋白和亲水性蛋白,二级结构以无规则卷曲为主。蛋白质保守基序分析显示,Motif1、Motif2所占的比例较高;系统进化树可将其分为9个亚族,大部分亚族中都包含蓖麻和拟南芥WRKY蛋白。基因表达差异分析发现,其中多数WRKY基因都至少在蓖麻发育中的一个时期表达;荧光定量分析结果显示,3个基因在叶中的表达量均低于在花和第50天种子中的表达量,RcWRKY31在叶中几乎不表达,且RcWRKY57在第25天种子中表达量相对较低,即同一基因家族在蓖麻叶、花、种子中的表达存在一定差异;启动子分析表明,WRKY基因家族启动子区含有12个响应生物胁迫和非生物胁迫的顺式作用元件。【结论】蓖麻中共检测到58个WRKY基因,能够参与复杂的抗逆反应以及生长发育调控过程,帮助蓖麻应对逆境胁迫及其自身生长带来的不利影响。

关键词: 蓖麻, WRKY基因, 转录因子, 生物信息学

Abstract: 【Objective】 To identify the members of WRKY transcription factor family of Ricinus communis,and provide a basis for further studies of the functions,evolution,and molecular breeding of the WRKY gene family members of R. communis. 【Methods】 Bioinformatics approaches were applied to analyze the physicochemical properties,secondary structures,conserved motifs,phylogenetic tree analysis,gene expression differences,and promoter cis-acting elements of proteins of WRKY transcription factor family members of R. communis. 【Results】 A total of 58 genes were identified in the WRKY gene family of R. communis. Analyses of physicochemical properties and secondary structure indicated that most proteins in WRKY transcription factor gene family were basic and hydrophilic proteins,and the secondary structure consists mainly of random coils. Protein conserved motif analysis showed that Motif1 and Motif2 accounted for a relatively high proportion. Phylogenetic tree analysis classified these proteins into nine subfamilies,and most subfamilies contained WRKY proteins of both R. communis and Arabidopsis thaliana. Gene expression difference analysis revealed that most of WRKY genes were expressed at least at one stage during R. communis development. Quantitative real-time analysis results indicated that the expression levels of three genes in leaves were lower than those in flowers and in seeds at 50 d. RcWRKY31 was almost not expressed in leaves,and RcWRKY57 showed relatively low expression in seeds at 25 d. These findings indicated that members of the same gene family exhibit certain differences in expression among R. communis leaves,flowers,and seeds. Promoter analysis showed that the promoter regions of WRKY gene family contain 12 cis-acting elements responsive to both biotic and abiotic stresses. 【Conclusion】 A total of 58 WRKY genes are identified in R. communis. These genes are involved in complex stress response and growth and development regulatory processes,thereby helping R. communis to cope with the adverse effects from environmental stresses and its own growth.

Key words: Ricinus communis, WRKY gene, Transcription factor, Bioinformatics

中图分类号:

S565.6

张春玲, 狄建军, 韩雯毓, 刘庆秀, 王冬艳, 曲毅鹏, 张斯琴, 何智彪. 蓖麻全基因组WRKY基因家族的鉴定及表达分析[J]. 北方农业学报, 2026, 54(2): 11-24.

ZHANG Chunling, DI Jianjun, HAN Wenyu, LIU Qingxiu, WANG Dongyan, QU Yipeng, ZHANG Siqin, HE Zhibiao. Identification and expression analysis of the WRKY gene family in the whole genome of Ricinus communis[J]. Journal of Northern Agriculture, 2026, 54(2): 11-24.

参考文献

[1] 李楠. 蓖麻毒蛋白的提取分离技术研究[D].长沙:中南林业科技大学,2014.
[2] 于思源. 癸二酸制备工艺改进的研究[D].太原:中北大学,2020.
[3] HAN B,WU D,ZHANG Y Y,et al.Epigenetic regulation of seed-specific gene expression by DNA methylation valleys in castor bean[J].BMC Biology,2022,20(1):57.
[4] XIE T,CHEN C J,LI C H,et al.Genome-wide investigation of WRKY gene family in pineapple:evolution and expression profiles during development and stress[J].BMC Genomics,2018,19(1):490.
[5] CUI X,ZHAO P Y,LIANG W W,et al.A rapeseed WRKY transcription factor phosphorylated by CPK modulates cell death and leaf senescence by regulating the expression of ROS and SA-synthesis-related genes[J].Journal of Agricultural and Food Chemistry,2020,68(28):7348-7359.
[6] FANG X,LIU L B,LI M R,et al.WRKY transcription factors modulate flowering time in four Arachis species:a bioinformatics analysis[J].BMC Plant Biology,2024,24(1):620.
[7] EULGEM T,RUSHTON P J,ROBATZEK S,et al.The WRKY superfamily of plant transcription factors[J].Trends in Plant Science,2000,5(5):199-206.
[8] WANG Q S,WANG M H,ZHANG X Z,et al.WRKY gene family evolution in Arabidopsis thaliana[J].Genetica,2011,139(8):973-983.
[9] 项玉婷,王景,曾俊岚,等.向日葵WRKY转录因子家族鉴定与生物信息学分析[J].分子植物育种,2020,18(14):4572-4586.
[10] BAILLO E H,HANIF M S,GUO Y H,et al.Genome-wide Identification of WRKY transcription factor family members in sorghum (Sorghum bicolor (L.) Moench)[J].PLoS One,2020,15(8):e0236651.
[11] WANG P J,YUE C,CHEN D,et al.Genome-wide identification of WRKY family genes and their response to abiotic stresses in tea plant (Camellia sinensis)[J].Genes & Genomics,2019,41(1):17-33.
[12] XUE C L,LI H T,LIU Z G,et al.Genome-wide analysis of the WRKY gene family and their positive responses to phytoplasma invasion in Chinese jujube[J].BMC Genomics,2019,20(1):464.
[13] CHEN L,YANG Y,LIU C,et al.Characterization of WRKY transcription factors in Solanum lycopersicum reveals collinearity and their expression patterns under cold treatment[J].Biochemical and Biophysical Research Communications,2015,464(3):962-968.
[14] BARANWAL V K,NEGI N,KHURANA P.Genome-wide identification and structural,functional and evolutionary analysis of WRKY components of mulberry[J].Scientific Reports,2016,6:30794.
[15] FELIPEZ W,DE FREITAS K E J,DOS SANTOS R S,et al.The roles of WRKY transcription factors in Malus spp.and Pyrus spp.[J].Functional & Integrative Genomics,2022,22(5):713-729.
[16] CHAI W B,SI W N,JI W,et al.Genome-wide investigation and expression profiling of HD-zip transcription factors in foxtail millet (Setaria italica L.)[J].BioMed Research International,2018,2018:8457614.
[17] WANG Z,ZHANG Z P,WANG P P,et al.Genome-wide identification of the NAC transcription factors family and regulation of metabolites under salt stress in Isatis indigotica[J].International Journal of Biological Macro-molecules,2023,240:124436.
[18] 曹丽茹,张前进,郭子宁,等.玉米生长素响应因子基因家族全基因组鉴定及表达分析[J].核农学报,2021,35(9):2016-2026.
[19] 未丽,刘建利.植物蛋白质亚细胞定位相关研究概述[J].植物科学学报,2021,39(1):93-101.
[20] TIAN Y,DONG Q L,JI Z R,et al.Genome-wide identification and analysis of the MADS-box gene family in apple[J].Gene,2015,555(2):277-290.
[21] WU K L,GUO Z J,WANG H H,et al.The WRKY family of transcription factors in rice and Arabidopsis and their origins[J].DNA Research,2005,12(1):9-26.
[22] TANG R N,ZHU Y J,YANG S M,et al.Genome-wide identification and analysis of WRKY gene family in melastoma dodecandrum[J].International Journal Molecular Sciences,2023,24(19):14904.
[23] GUO C L,GUO R R,XU X Z,et al.Evolution and expression analysis of the grape (Vitis vinifera L.) WRKY gene family[J].Journal of Experimental Botany,2014,65(6):1513-1528.
[24] MAEO K,HAYASHI S,KOJIMA-SUZUKI H,et al.Role of conserved residues of the WRKY domain in the DNA-binding of tobacco WRKY family proteins[J].Bioscience,Biotechnology and Biochemistry,2001,65(11):2428-2436.
[25] RIECHMANN J L,RATCLIFFE O J.A genomic perspective on plant transcription factors[J].Current Opinion in Plant Biology,2000,3(5):423-434.
[26] CHEN F,HU Y,VANNOZZI A,et al.The WRKY transcription factor family in model plants and crops[J].Critical Reviews in Plant Sciences,2017,36(5/6):311-335.
[27] YIN Y Y,FU H H,MI F K,et al.Genomic characterization of WRKY transcription factors related to secoiridoid biosynthesis in Gentiana macrophylla[J].BMC Plant Biology,2024,24(1):66.
[28] JIANG W B,YU D Q.Arabidopsis WRKY2 transcription factor mediates seed germination and postgermination arrest of development by abscisic acid[J].BMC Plant Biology,2009,9(1):96.
[29] LEI P,LIU Z,HU Y B,et al.Transcriptome analysis of salt stress responsiveness in the seedlings of wild and cultivated Ricinus communis L.[J].Journal of Biotechnology,2021,327:106-116.
[30] ZHOU Q,GUO Z,ZHOU X,et al.Genome-wide identification and characterization of the WRKY gene family in Cucurbita maxima[J].Genes,2023,14(11):2030.

蓖麻全基因组WRKY基因家族的鉴定及表达分析

Identification and expression analysis of the WRKY gene family in the whole genome of Ricinus communis

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	陈子怡, 汪晓蕊, 何涵, 李子墨, 夏自玉, 黄文静, 孙晓春. 桔梗bHLH基因家族鉴定与表达分析[J]. 北方农业学报, 2026, 54(1): 43-55.
[2]	龙金佳, 徐劲松. 苗期甘蓝型油菜长期受渍下的转录组分析[J]. 北方农业学报, 2025, 53(6): 26-42.
[3]	李明静, 何智彪, 乔文杰, 贾娟霞, 莫德乐吐, 张智勇, 雷凤燕, 王国成, 张晓华, 黄凤兰. 蓖麻饼粕肥对欧李土壤养分和酶活性的影响[J]. 北方农业学报, 2025, 53(3): 35-46.
[4]	裴洪伟, 索佳慧, 武海娜, 孙正文, 张艳, 杨君. 陆地棉CRK基因家族抗黄萎病功能分析[J]. 北方农业学报, 2024, 52(6): 1-12.
[5]	段强, 何智彪, 李国瑞, 赵秀平, 张帅, 韩雯毓, 陈永胜. 蓖麻LBD基因家族全基因组鉴定、进化和表达分析[J]. 北方农业学报, 2021, 49(6): 1-14.
[6]	房永雨, 郝丽芬, 聂利珍, 丁海君, 王力伟, 赵小庆, 孙杰, 史志丹, 何江峰. 沙拐枣甜菜碱醛脱氢酶基因的克隆与生物信息学分析[J]. 北方农业学报, 2020, 48(3): 32-38.
[7]	邱凯华, 李晓驹, 方淑梅, 梁喜龙. 9种真菌中分支点结合蛋白的生物信息学分析[J]. 北方农业学报, 2020, 48(3): 39-46.
[8]	朱长保;刘志;徐辰峰;刘仁梅;林中正;成立瑞. 全基因组分析盐胁迫下水稻转录因子的表达[J]. 北方农业学报, 2018, 46(6): 38-43.
[9]	贾娟霞;朱国立;张智勇;何智彪;莫德乐吐;乔文杰. 矮秆蓖麻杂交种通蓖13号选育及栽培技术要点[J]. 北方农业学报, 2018, 46(5): 35-38.
[10]	苏和杨建强柴绍忠张学英布和巴特尔俞璐. 阿拉善左旗蓖麻栽培密度及播种期试验[J]. 北方农业学报, 2015, 43(2): 9-9.
[11]	雷雪黄凤兰孟凡娟罗蕊陈晓凤赵永李跃孙华军. 单粒蓖麻种子毒蛋白含量测定方法的研究[J]. 北方农业学报, 2015, 43(2): 5-5.
[12]	张智勇倪娜王建朱国立莫德乐吐乔文杰贾娟霞何智彪甘茂杰. 蓖麻主穗开花期的主基因＋多基因混合遗传分析[J]. 北方农业学报, 2015, (1): 10-10.
[13]	包春光;黄凤兰;朱国立;何智彪;彭木;陈晓凤;罗蕊;赵永. 蓖麻种子性状的研究进展[J]. 北方农业学报, 2014, (6): 102-102.
[14]	黄凤兰朱国立潘微彤何智彪包春光彭木王文跃张智勇张现世. 蓖麻3种类型花序发育过程的显微学比较研究[J]. 北方农业学报, 2014, (4): 10-10.
[15]	金伊洙白佳鹭沈力. 不同品种蓖麻花粉生活力特性的研究[J]. 北方农业学报, 2014, (3): 10-10.