耐低温西瓜种质筛选及低温对西瓜生理指标的影响

doi:10.12190/j.issn.2096-1197.2021.02.18

Abstract

Abstract: 【Objective】To screen watermelon germplasm materials with low temperature tolerance,explore breeding physiological indexes,and provide materials and theoretical reference for breeding of low temperature tolerant watermelon varieties.【Methods】Using 30 watermelon varieties（lines） as experimental materials,the effects of low temperature stress on the chilling injury index and recovery index of watermelon seedlings were analyzed by simulating low temperature stress（7.5 ℃） in an incubator. Furthermore,Zaojia 8424 and Qianli No.4 were selected as the test materials to analyze the effects of long-term low temperature stress （8 ℃ for 15 days）,short-term low temperature stress （4 ℃ for 2 days） and normal temperature recovery growth（28/22 ℃ for 5 days） on antioxidant enzyme activity,malondialdehyde content,reactive oxygen species content and osmotic adjustment substance content of watermelon seedlings.【Results】There were significant differences（P<0.05） in chilling injury index and no significant difference（P>0.05） in recovery index among different watermelon materials under low temperature stress of 7.5 ℃. Five materials （Qianli No.2,Qianli No.3,Qianli No.4,18S-135-2 and 18S-145-1） selected from middle fruit type had significantly （P<0.05） stronger tolerance to low temperature than Zaojia 8424（CK1）. The ability of low temperature tolerance of 2 materials（17A-79×17A-82 and 17A-82×17A-79） selected from small fruit type was stronger than that of Xiaolan（CK2）. The changes of antioxidant enzyme activities,osmotic adjustment substance content and reactive oxygen species content of Zaojia 8424 and Qianli No.4 seedlings were different under different low temperature treatment（short-term low temperature stress and long-term low temperature stress）. Qianli No.4 had higher antioxidant enzyme activity,soluble protein content and soluble sugar content,and lower content of malondialdehyde,superoxide anion（O₂^-） and hydrogen peroxide（H₂O₂）,the content of proline of Zaojia 8424 and Qianli No.4 varies with different low temperature treatments.【Conclusion】Thirty watermelon varieties （lines） had different tolerance and physiological response to low temperature stress. Low temperature tolerant germplasm materials were Qianli No.2,Qianli No.3,Qianli No.4,18S-135-2,18S-145-1,17A-79×17A-82 and 17A-82×17A-79.

Key words: Watermelon, Germplasm, Physiological index, Low temperature stress

CLC Number:

S651

SHEN Hong, MENG Jiali, WU Shaojun, YU Xiang. Screen of low temperature tolerant germplasm and the effect of low temperature on the physiological indexes in watermelon[J].Journal of Northern Agriculture, 2021, 49(2): 115-123.

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References

[1] 彭金光,孙玉宏,师瑞红,等.不同西瓜品种萌发期耐冷性的综合比较分析[J].安徽农学通报,2006(11):47-49.
[2] 李严曼,朱磊,朱华玉,等.不同西瓜品种苗期耐低温性差异研究[J].河南农业大学学报,2016,50(5):604-608.
[3] 邢燕,徐志红,徐永阳,等.西瓜苗期耐低温弱光性鉴定指标初探[J].中国瓜菜,2012,25(6):31-33.
[4] 王薇薇,羊杏平,范淑英,等.西瓜耐冷性鉴定指标的筛选[J].华北农学报,2014,29(6):163-171.
[5] 冯素伟,董娜,胡铁柱,等.不同小麦品种抗低温能力比较[J].河南科技学院学报(自然科学版),2010,38(3):4-7.
[6] 任旭琴,曹碚生,缪旻珉,等.辣椒不同生育期耐低温性鉴定及相关分析[J].安徽农业大学学报,2010,37(1):141-144.
[7] 郭数进,李玮瑜,马艳芸,等.山西不同生态型大豆品种苗期耐低温性综合评价[J].植物生态学报,2014,38(9):990-1000.
[8] 许勇,王永健,张峰,等.西瓜幼苗耐低温研究初报[J].华北农学报,1997,12(2):93-96.
[9] 王学奎. 植物生理生化试验原理和技术[M].北京:高等教育出版社,2006.
[10] 江福英,李延,翁伯琦.植物低温胁迫及其抗性生理[J].福建农业学报,2002(3):190-195.
[11] 王萍,吕星光,李敏.低温胁迫对西瓜砧木幼苗生长和生理指标的影响[J].北方园艺,2017(10):35-38.
[12] 李爱民,张永泰,熊飞,等.低温弱光对西瓜幼苗生理特性的影响[J].扬州大学学报(农业与生命科学版),2012,33(2):78-82.
[13] 杨燕. 低温胁迫对籽用西瓜幼苗生长及生理生化特性的影响[D].呼和浩特:内蒙古农业大学,2012.
[14] 段会军,李喜焕,吴立强,等.低温胁迫下西瓜幼苗生理特性与冷害的关系[J].中国农学通报,2007(1):84-87.
[15] 韩娅楠,米国全,史艳艳,等.低温对野生番茄和栽培番茄叶片抗氧化代谢和碳同化的影响[J].华北农学报,2018,33(3):153-158.
[16] 张爱华,王静华,李艳梅,等.西瓜种质资源耐低温性评价[J].中国农学通报,2010,26(18):219-223.

Screen of low temperature tolerant germplasm and the effect of low temperature on the physiological indexes in watermelon

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