沙芥果实精油抗氧化及抑菌活性的研究

doi:10.12190/j.issn.2096-1197.2021.04.17

Abstract

Abstract: 【Objective】To clarify the antioxidant and antibacterial activities of essential oil from the fruit of Pugionium cornutum,and to provide reference for further development and utilization of essential oil products from the fruit of Pugionium cornutum.【Methods】The antioxidant activity of essential oil from the fruit of Pugionium cornutum was analyzed by measuring the scavenging of ·OH amd ·O₂^-and reducing capacities of different concentrations of the fruit of Pugionium cornutum diluents. At the same time,the antibacterial activity of essential oil from the fruit of Pugionium cornutum against Staphylococcus aureus,Escherichia coli,Bacillus subtilis,Listeria monocytogenes,Saccharomyces cerevisiae,Candida lipolitica and Aspergillus niger were detected.【Results】The antioxidative activity of essential oil from the fruit of Pugionium cornutum was strong,and it increased with the increase of the concentration of essential oil from the fruit of Pugionium cornutum. The scavenging ·O₂^- ability of essential oil from the fruit of Pugionium cornutum was better than vitamin C（VC）and 2,6-di-tert-butyl-4-methylphenol（BHT）,but the reducing ability was lower than VC and BHT. The essential oil from the fruit of Pugionium cornutum had strong antibacterial activities against Staphylococcus aureus,Escherichia coli,Listeria monocytogenes,Bacillus subtilis,Aspergillus niger,Saccharomyces cerevisiae and Candida alpium. The minimum inhibitory concentration（MIC）against Staphylococcus aureus,Escherichia coli and Listeria monocytogenes was 40 mg/mL. The MIC was 30 mg/mL for Bacillus subtilis,8 mg/mL for Aspergillus niger,Saccharomyces cerevisiae and Candida albatum. The inhibition zone diameter of Listeria monocytogenes was 15.34 mm,which was high-sensitive type. The inhibition zone diameters of Escherichia coli,Aspergillus niger,Saccharomyces cerevisiae and Staphylococcus aureus were 14.29,14.00,11.26 and 10.20 mm,respectively,which was moderate-sensitive type. The diameters of inhibition zone of Bacillus subtilis and Candida albicans were 9.70 mm and 7.69 mm,respectively,which was low-sensitive type. The order of inhibitory effect of essential oil from the fruit of Pugionium cornutum on the tested bacteria was Listeria monocytogenes>Escherichia coli>Aspergillus niger>Saccharomyces cerevisiae>Staphylococcus aureus>Bacillus subtilis>Candida lipolitica.【Conclusion】The essential oil from the fruit of Pugionium cornutum had strong antioxidant activity and broad-spectrum antibacterial activity,which was a kind of natural plant material with good prospect for development and utilization.

Key words: Pugionium cornutum, Fruit, Essential oils, Antioxidant activity, Antimicrobial activity

CLC Number:

S647

LI Na, JIA Lixia, FU Nana, HAO Lizhen, HUANG Xiumei, ZHANG Fenglan. Study on antioxidant and antibacterial activity of essential oil from the fruit of Pugionium cornutum[J].Journal of Northern Agriculture, 2021, 49(4): 120-126.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2021.04.17

https://journal30.magtechjournal.com/bfnyxb/EN/Y2021/V49/I4/120

References

[1] SADGROVE N,JONES G.A contemporary introduction to essential oils:chemistry,bioactivity and prospects for australian agriculture[J].Agriculture,2015,5(1):48-102.
[2] 邓奇风,蒋载阳,高凤仙.植物精油的生理功能及其在饲料中的应用[J].广东饲料,2016,25(4):37-39.
[3] 任澎,范宁,田淼,等.植物精油药物作用研究进展[J].中华中医药杂志,2018,33(6):2507-2511.
[4] 吴克刚,罗敏婷,魏浩.8种植物精油对肠道常见微生物体外抑菌效果的研究[J].现代食品科技,2017,33(6):133-141.
[5] 柴向华,董艳,吴克刚,等.植物精油对食品中常见有害微生物的抑菌活性研究[J].现代食品科技,2016,32(8):123-127.
[6] PRAKASH B,KEDIA A,MISHRA P K,et al.Plant essential oils as food preservatives to control moulds,mycotoxin contamination and oxidative deterioration of agri-food commodities -potentials and challenges[J].Food Control,2015,47:381-391.
[7] 周旭. 脂溶性天然抗氧化剂在葡萄籽油和核桃油中的应用研究[D].杭州:浙江大学,2016.
[8] 梁振益,吴娟.薄荷提取物抗氧化性能的研究[J].中国油脂,2008,33(2):26-28.
[9] REICHLING J,SCHNITZLER P,SUSCHKE U,et al.Essential oils of aromatic plants with antibacterial,antifungal,antiviral,and cytotoxic properties—an overview[J].Forsch Komplementmed,2009,16:79-90.
[10] 徐娟娟,黎卓熹,关冠恒,等.两种肉桂精油的化学成分与抗氧化活性研究[J].四川师范大学学报(自然科学版),2020,43(6):827-837.
[11] 刘书成,李元瑞,王丽华,等.大蒜精油对食用油脂的抗氧化特性[J].食品科学,2002,23(1):128-131.
[12] 张鑫,耿庆何,郭超,等.植物精油在粮油食品抑菌中的应用[J].粮油食品科技,2021,29(1):180-186.
[13] 张凤兰,杨忠仁,郝丽珍,等.5种野生蔬菜叶片营养成分分析[J].华北农学报,2009,24(2):164-180.
[14] LI H Y,LI C,ZHANG C X,et al.Compositional and gastrointestinal prokinetic studies of Pugionium(L.)[J].Food Chemistry,2015,186:285-291.
[15] 江苏新医学院.中药大辞典[M].上海:上海人民出版社,1977:1162.
[16] 张凤兰,宁可真,杨忠仁,等.沙芥新品种沙珍J-2号[J].中国蔬菜,2020(11):120-122.
[17] 黄修梅,郝丽珍,王怀栋,等.沙芥和斧形沙芥种子萌发对土壤含水量的适应性[J].西北植物学报,2012,32(2):355-361.
[18] 庞杰,郝丽珍,张凤兰,等.沙芥叶片活性氧和抗坏血酸对干旱胁迫的响应[J].植物生理学报,2013,49(1):57-62.
[19] 郑清岭,杨忠仁,张晓艳,等.干旱胁迫对沙芥和斧形沙芥抗坏血酸含量及其代谢相关酶的影响[J].植物生理学报,2018,54(12):1865-1874.
[20] WANG P,WANG F F,YANG J.De novo assembly and analysis of the Pugionium cornutum(L.) Gaertn.transcriptome and identification of genes involved in the drought response[J].Gene,2017,626:290-297.
[21] CUI Y N,WANG F Z,YANG C H,et al.Transcriptomic profiling identifies candidate genes involved in the salt tolerance of the Xerophyte Pugionium cornutum[J].Gene,2019,10(12):1039-1052.
[22] OBOH G,PUNTEL R L,ROCHA J B T.Hot pepper(Capsicum annuum,Tepin and Capsicum chinese, Habanero) prevents Fe²+-induced lipid per oxidation in brain—in vitro[J].Food Chemistry,2007,102:178-185.
[23] FERRERES F,SOUSA C,VRCHOVSKÁ V,et al.Chemical composition and antioxidant activity of tronchuda cabbage internal leaves[J].European Food Research & Technology,2006,222:88-89.
[24] RAGHAVENDRA H,LAKSHMANASHETTY V B,NAGARAJ M G,et al.In vitro antioxidant activity of Vitex negundo L. leaf extracts[J].Chiang Mai Journal of Science,2010,37(3):489-497.
[25] 唐裕芳,张妙玲,陶能国,等.苍术挥发油的提取及其抑菌活性研究[J].西北植物学报,2008,28(3):588-594.
[26] 张存莉,朱玮,王冬梅,等.黑刺菝葜根提取物的抑菌活性研究[J].西北植物学报,2005,25(12):2529-2532.
[27] 曾琳,林秋美,韩成云,等.香榧叶精油的化学成分、抗氧化及抑菌活性的研究[J].中国粮油学报,2020,35(2):98-104.
[28] GUERRA I C D,DE OLIVEIRA P D L,DE SOUZAPON-TES A L,et al.Coatings comprising chitosan and Mentha piperita L. or mentha× villosahuds essential oils to prevent common postharvest mold infections and maintain he quality of cherry tomato fruit[J].International Journal of Food Microbiology,2015,214(2):168-178.
[29] MAJDINASAB M,NIAKOUSARI M,SHAGHAGHIAN S,et al.Antimicrobial and antioxidant coating based on basil seed gum incorporated with shirazi thyme and summer savory essential oils emulsions for shelf-life extension of refrigerated chicken fillets[J].Food Hydrocolloids,2020,108:106011-106022.
[30] RISALITI L,KEHAGIA A,DAOULTZI E,et al.Liposomes loaded with salvia triloba and rosmarinus officinalis essential oils:in vitro assessment of antioxidant,antiinflammatory and antibacterial activities[J].Journal of Drug Delivery Science and Technology,2019,51:493-498.
[31] 刘星. 百里香精油提取物、抗氧化活性、护肝作用及其应用的研究[D].西安:陕西师范大学,2018.
[32] 高海荣,黄振旭,李霞,等.河南产白蒿精油的化学成分分析及抑菌活性研究[J].食品研究与开发,2020,41(7):69-74.

Study on antioxidant and antibacterial activity of essential oil from the fruit of Pugionium cornutum

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